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Author Iwata, Yasunori| Satou, Kenji| Furuichi, Kengo| Yoneda, Ikuko| Matsumura, Takuhiro| Yutani, Masahiro| Fujinaga, Yukako| Hase, Atsushi| Morita, Hidetoshi| Ohta, Toshiko| Senda, Yasuko| Sakai-Takemori, Yukiko| Wada, Taizo| Fujita, Shinichi| Miyake, Taito| Yasuda, Haruka| Sakai, Norihiko| Kitajima, Shinji| Toyama, Tadashi| Shinozaki, Yasuyuki| Sagara, Akihiro| Miyagawa, Taro| Hara, Akinori| Shimizu, Miho| Kamikawa, Yasutaka| Ikeo, Kazuho| Shichino, Shigeyuki| Ueha, Satoshi| Nakajima, Takuya| Matsushima, Kouji| Kaneko, Shuichi| Wada, Takashi|
Keywords Bloodstream infection Cna MRSA Whole genome sequencing
Published Date 2019-11-15
Publication Title International Journal of Infectious Diseases
Volume volume91
Publisher Elsevier
Start Page 22
End Page 31
ISSN 12019712
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders © 2019 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
File Version publisher
PubMed ID 31740408
DOI 10.1016/j.ijid.2019.11.003
Web of Science KeyUT 000510867100004
Related Url isVersionOf https://doi.org/10.1016/j.ijid.2019.11.003
JaLCDOI 10.18926/AMO/31612
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Author Funamori, Yuka| Fujinaga, Yukako| Yokota, Kenji| Inoue, Kaoru| Hirai, Yoshikazu| Oguma, Keiji| Kira, Shohei| Taketa, Kazuhisa|
Abstract <p>Three outbreaks and many isolated cases of enterohemorrhagic Escherichia coli O157:H7 occurred in 1996 and 1997 in Okayama Prefecture, Japan. In an attempt to investigate the route of these infections, the strains isolated from the 3 outbreaks (total 33 strains) and 15 isolated cases (total 15 strains) were investigated using random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE). In addition, 10 strains from an outbreak in Tojo Cho, Hiroshima Prefecture (June 1996), 2 strains from the particular types of meat in Kochi Prefecture, and 42 strains isolated from bovine feces in a farm in Okayama Prefecture were also investigated in the same manner. PFGE was much more useful than RAPD for molecular typing of the clinical isolates, in that it allowed us to classify them into 10 PFGE groups. We noted that the strains differed according to the time and place of the outbreaks (or isolated cases). This indicates that O157:H7 infections in Okayama Prefecture were caused by different strains (although some cases were aggravated by the same strains as were found in other areas). The isolates from bovine feces were classified into 5 groups by PFGE profiles, but none of them were identical to those of the clinical isolates.</p>
Keywords molecular epidemiology enterohemorrhagic Escherichia coli O157: H7 pulsed field gel electrophoresis random amplification of polymorphic DNA
Amo Type Article
Published Date 1999-08
Publication Title Acta Medica Okayama
Volume volume53
Issue issue4
Publisher Okayama University Medical School
Start Page 193
End Page 200
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10488407
Web of Science KeyUT 000082334300006