Author Kanemasa, Yasuhiro| Takai, Ken-ichi| Takatsu, Tieko| Hayashi, Hideo| Katayama, Takeshi|
Published Date 1974-10
Publication Title Acta Medica Okayama
Volume volume28
Issue issue5
Content Type Journal Article
Author Hayashi, Hideo|
Published Date 1969-08
Publication Title Acta Medicinae Okayama
Volume volume23
Issue issue4
Content Type Journal Article
Author Seki, Shuji| Yamamoto, Goki| Hayashi, Hideo| Inohara, Risaburo| Oda, Takuzo|
Published Date 1967-08
Publication Title Acta Medicinae Okayama
Volume volume21
Issue issue4
Content Type Journal Article
Author Tsutsui, Ken| Koide, Noriko| Tomoda, Jun| Hayashi, Hideo| Hatase, Osamu| Oda, Takuzo|
Published Date 1977-10
Publication Title Acta Medica Okayama
Volume volume31
Issue issue5
Content Type Journal Article
JaLCDOI 10.18926/AMO/30338
FullText URL fulltext.pdf
Author Minami, Junzabro| Okabe, Akinobu| Nagata, Akihide| Hayashi, Hideo|
Abstract <p>A modified method of passive immune hemolysis (PIH) was applied to the quantitative assay of heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. The method enabled the measurement of 0.2 to 1.2 ng LT. The production of LT by enterotoxigenic E. coli under various conditions was analyzed using the modified method. LT production was intense during the logarithmic growth phase and decreased during the stationary growth phase. Lincomycin (50 to 100 micrograms/ml) affected cell growth slightly, but enhanced production of LT until the late-stationary growth phase. About 90% of the LT produced was retained in the cell, and the rest was excreted into the culture medium. The initial pH of the culture medium affected LT production. Alkaline pH enhanced LT production, though growth was depressed. Aeration enhanced both growth and LT production.</p>
Keywords heat-labile enterotoxin E coli passive immune hemolysis
Amo Type Article
Published Date 1984-10
Publication Title Acta Medica Okayama
Volume volume38
Issue issue5
Publisher Okayama University Medical School
Start Page 461
End Page 469
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6393717
Web of Sience KeyUT A1984TQ61100006