JaLCDOI 10.18926/AMO/31536
FullText URL fulltext.pdf
Author Hashimoto, Kozo| Hattori, Teruhiko| Murakami, Kazuharu| Suemaru, Shouso| Kawada, Yoshiro| Ota, Zensuke|
Abstract <p>The stability of rat (human) CRF in serum, urine and tissue incubation medium was examined using Sephadex gel filtration and CRF radioimmunoassay with anti-rat (human) CRF serum. Human serum after incubation with rat (human) CRF for 1 h at 37 degrees C showed two peaks of CRF immunoreactivity on a Sephadex G-50 fine column. Most of the immunoreactivity coeluted with the rat (human) CRF marker. When rat (human) CRF was incubated with rat liver, kidney or hypothalamus, only 3.1-14.9% of the CRF was recovered at the rat (human) CRF position on gel filtration, and two to four CRF-immunoreactive peaks appeared after the rat (human) CRF marker. When rat (human) CRF was incubated with human urine (pH 6.0) for 24 h at room temperature, one peak of CRF immunoreactivity coeluted with the rat (human) CRF marker on Sephadex gel filtration. The urine extracts of normal rats showed some small peaks of CRF-like immunoreactivity on the Sephadex column, with the main peak appearing after authentic CRF. These results suggest that rat (human) CRF is relatively stable in serum and urine, but is easily degraded by tissue enzymes, with the degraded CRF fragments being excreted in the urine.</p>
Keywords rat(human) corticotropin releasing factor sephadex chromatography stability
Amo Type Article
Published Date 1985-06
Publication Title Acta Medica Okayama
Volume volume39
Issue issue3
Publisher Okayama University Medical School
Start Page 199
End Page 206
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3875214
Web of Science KeyUT A1985ALG3300005
JaLCDOI 10.18926/AMO/30349
FullText URL fulltext.pdf
Author Hashimoto, Kozo| Murakami, Kazuharu| Hattori, Teruhiko| Ota, Zensuke|
Abstract <p>Plasma immunoreactive CRF measured by radioimmunoassay decreased rapidly after intravenous injection of synthetic ovine corticotropin releasing factor (CRF) and showed a bi-exponential decay curve in five macaca fuscatas. Half lives of plasma immunoreactive CRF were 5.8 +/- 1.4 (Mean +/- SEM) min for the fast component and 38.3 +/- 2.4 min for the slow component. A bolus injection of 5 micrograms/kg CRF significantly increased the plasma cortisol level. CRF at 5 micrograms/kg induced a delayed response of ACTH and cortisol. Arginine vasopressin (AVP) at 0.5 micrograms/kg induced a slight increase in plasma ACTH and cortisol, but AVP at 0.1 micrograms/kg evoked no significant increase. When 0.5 micrograms/kg CRF and 0.1 micrograms/kg AVP were administered simultaneously, significant ACTH and cortisol responses occurred. The results indicate that CRF and AVP act synergistically to stimulate ACTH secretion in vivo.</p>
Keywords corticotropin releasing factor arginine vasopressin adrenocorticotropin cortisol macaca fuscata
Amo Type Article
Published Date 1984-06
Publication Title Acta Medica Okayama
Volume volume38
Issue issue3
Publisher Okayama University Medical School
Start Page 261
End Page 267
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6087620
Web of Science KeyUT A1984SY25800006