JaLCDOI 10.18926/AMO/53677
FullText URL 69_5_307.pdf
Author Wada, Nozomu| Nouso, Kazuhiro| Kariyama, Kazuya| Wakuta, Akiko| Kishida, Masayuki| Nishimura, Mamoru| Higashi, Toshihiro|
Abstract Sarcoidosis is a systemic disease characterized by the formation of non-caseating granulomas in multiple organs. In the diagnosis of sarcoidosis, imaging modalities such as ultrasonography, computed tomography (CT) and magnetic resonance imaging (MRI) are useful;however, there are few reports of MRI imaging using gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB) MRI. A 46-year-old Japanese female with suspected pulmonary sarcoidosis was admitted to our hospital because low-density mottles in the liver were observed incidentally by chest CT. The low-density mottles were not enhanced at the arterial phase or portal phase by abdominal CT and MRI, and decreased uptake was observed in the hepatobiliary phase of Gd-EOB MRI. No hematological disorder was observed except for a slight increase of biliary enzymes. The lesion was diagnosed as liver sarcoidosis by the liver biopsy. Since the patient refused steroid therapy, we prescribed ursodeoxycholic acid (UDCA). 600mg/day. The serum levels of biliary enzymes were normalized and the abdominal CT findings gradually improved after the initiation of UDCA medication. Gd-EOB MRI showed unique hypointense areas in the liver at the hepatobiliary phase, which might be useful in the diagnosis of liver sarcoidosis.
Keywords liver sarcoidosis ursodeoxycholic acid (UDCA) gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB)
Amo Type Case Report
Published Date 2015-10
Publication Title Acta Medica Okayama
Volume volume69
Issue issue5
Publisher Okayama University Medical School
Start Page 307
End Page 311
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2015 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 26490028
Web of Science KeyUT 000365519600007
JaLCDOI 10.18926/AMO/48568
FullText URL 66_3_279.pdf
Author Nishimura, Mamoru| Nouso, Kazuhiro| Kariyama, Kazuya| Wakuta, Akiko| Kishida, Masayuki| Wada, Nozomu| Higashi, Toshihiro| Yamamoto, Kazuhide|
Abstract The artificial ascites technique is often used during radiofrequency ablation (RFA) for hepatocellular carcinoma (HCC) treatment because it prevents visceral damage and improves visualization by minimizing interference of the lungs and mesentery. This study determined the efficacy and safety of RFA using the artificial ascites technique in HCC patients. We examined 188 HCC patients who were treated by RFA and fulfilled the Milan criteria. Treatment outcomes (complete ablation rate, local recurrence rate, complication rate, liver function including total bilirubin level, alanine aminotransferase level, albumin level, and prothrombin time) were compared among patients divided into 3 groups based on the volume of artificial ascites injected:GroupⅠ (n=86), no artificial ascites injected;GroupⅡ (n=35), <1,000ml artificial ascites injected;and Group Ⅲ (n=67), >1,000ml artificial ascites injected. No significant difference was observed in complete ablation or local recurrence rates among the 3 groups, or in the extent of liver function damage after RFA. Artificial ascites disappeared within 7 days; additional diuretics were needed only in 5 (all from Group Ⅲ) of 102 patients. No serious complications such as intestinal perforation or intraperitoneal bleeding were observed. Thus, we found that artificial ascites injection during RFA is effective and safe, and can be used to prevent major procedural complications.
Keywords radiofrequency ablation hepatocellular carcinoma artificial ascites
Amo Type Original Article
Published Date 2012-06
Publication Title Acta Medica Okayama
Volume volume66
Issue issue3
Publisher Okayama University Medical School
Start Page 279
End Page 284
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2012 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 22729109
Web of Science KeyUT 000305669700012
JaLCDOI 10.18926/AMO/32651
FullText URL fulltext.pdf
Author Sato, Atsuhiko| Higashi, Toshihiro| Ling, Liu| Shiota, Tetsuya| Tsuji, Takao|
Abstract <p>Indocyanine green (ICG) was injected into rat liver nodules induced by 2-acetylaminofluorene (2-AAF) via portal vein. The relationship between ICG staining and cell atypism of liver nodules was examined by means of histology and DNA flow cytometry. After 2-AAF administration, many small nodules appeared on the liver surface. All hyperplastic nodules were ICG stained until 10 weeks after the administration, but some nodules were not stained after 14 weeks. ICG-stained nodules histologically consisted of benign tissues and borderline lesions, and many of them showed &#34;diploidy&#34; in DNA cytometry. ICG-unstained nodules consisted of hepatocellular carcinoma (HCCs) and borderline lesions, and many of them showed &#34;aneuploidy&#34;. In this way, it has been suggested that HCC could derive from hyperplastic nodules and that they might lose an ability to take up ICG in the process of hepatocarcinogenesis. Immunohistochemical staining for glutathione-S-transferase alpha (GST-alpha), a carrier protein of ICG in hepatocytes, was well correlated with ICG staining in the nodules, suggesting that the loss of ICG uptake in HCC was partly due to the decrease of GST-alpha. Moreover, the appearance of ICG unstained and aneuploid nodules was significantly inhibited in rats which were fed on diet containing Syosaiko-to after the administration of 2-AAF. Chemopreventive effect of Syo-saiko-to on hepatocarcinogenesis was identified.</p>
Keywords hepatocellular carcinoma idocyanine green carcinogenesis DNA flow cytometry Syo-saiko-to glutathione-S-transferase
Amo Type Article
Published Date 1992-04
Publication Title Acta Medica Okayama
Volume volume46
Issue issue2
Publisher Okayama University Medical School
Start Page 57
End Page 66
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1374211
Web of Science KeyUT A1992HR48400001
JaLCDOI 10.18926/AMO/32047
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Higashi, Toshihiro| Nagashima, Hideo|
Abstract <p>A reproducible animal model of fulminant hepatic failure was developed by intraperitoneal administration of D-galactosamine hydrochloride to Sprague-Dawley rats. Biochemical and morphological hepatic injury and brain edema resembled human fulminant hepatic failure. This model would facilitate further studies of the pathogenesis of brain dysfunction and evaluation of treatment in fulminant hepatic failure.</p>
Keywords fulminant hepatic failure brain edema massive liver injury hepatic encephalopathy
Amo Type Article
Published Date 1979-12
Publication Title Acta Medica Okayama
Volume volume33
Issue issue6
Publisher Okayama University Medical School
Start Page 443
End Page 450
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 161469
NAID 120002311355
JaLCDOI 10.18926/AMO/32038
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Higashi, Toshihiro| Endo, Hiroshi| Nagashima, Hideo|
Abstract <p>Three patients at various stages of remission from leukemia died following the development of massive liver necrosis within only 4-6 days. All had either hepatitis B surface antigen or antibody in their sera, and two of them experienced severe epigastric pain before the onset of liver injury. Hepatitis B surface antigen appeared in two of these patients after remission from leukemia. Serum gamma-globulin levels increased with decreasing doses of prednisolone and other antileukemic drugs, and hepatic cell necrosis occurred extensively. Localization of hepatitis B surface antigen in their livers revealed a strong positive reaction in the phagocytic cells. These observations strongly suggest that hepatitis B virus may be causally related to the fulminant hepatic failure at least in two of the reported leukemic patients.</p>
Keywords fulminant hepatic failure leukemia hepatitis B surface antigen massive liver necrosis immunosuppressive agents
Amo Type Article
Published Date 1979-08
Publication Title Acta Medica Okayama
Volume volume33
Issue issue4
Publisher Okayama University Medical School
Start Page 245
End Page 257
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 158950
JaLCDOI 10.18926/AMO/32003
FullText URL fulltext.pdf
Author Yoshida, Yasuhiro| Higashi, Toshihiro| Nouso, Kazuhiro| Nakatsukasa, Harushige| Nakamura, Shin-ichiro| Watanabe, Akiharu| Tsuji, Takao|
Abstract <p>Hepatic encephalopathy is one of the major complications in decompensated liver cirrhosis. The current study was conducted to clarify the mechanisms of zinc deficiency in liver cirrhosis and its involvement in hepatic encephalopathy via ammonia metabolism. Ten patients each with compensated or decompensated liver cirrhosis and 11 healthy volunteers were enrolled in the study. Serum zinc levels and its daily urinary excretion were measured, an oral zinc-tolerance test was performed to examine zinc malabsorption, and the effects of diuretics on zinc excretion and of zinc supplementation on ammonia metabolism in the skeletal muscle were studied. The mean serum zinc levels in patients with decompensated liver cirrhosis were found to be significantly lower than the levels in controls and patients with compensated liver cirrhosis. The serum zinc levels were inversely correlated with blood ammonia in the fasting state. In the oral zinc-tolerance test, the percent increase in serum zinc levels 120 and 180 min after ingestion was less in cirrhotic patients than in controls. A diuretic administration resulted in a significant reduction in serum zinc levels. An increased uptake of ammonia by and an increased release of glutamine from leg skeletal muscle after oral supplementation of zinc sulfate were evident. Taken together, zinc deficiency in decompensated cirrhotic patients appears to be due to low absorption and to high urinary excretion, for which excessive diuretic administration is, in part, responsible, and zinc supplementation might play an important role in the prevention of hepatic encephalopathy by activating glutamine synthetase.</p>
Keywords zinc ammonia liver cirrhosis hepatic encephalopathy
Amo Type Article
Published Date 2001-12
Publication Title Acta Medica Okayama
Volume volume55
Issue issue6
Publisher Okayama University Medical School
Start Page 349
End Page 355
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11779097
Web of Science KeyUT 000172838400005
JaLCDOI 10.18926/AMO/31944
FullText URL fulltext.pdf
Author Higashi, Toshihiro| Hashimoto, Makoto| Watanabe, Masatomo| Yamauchi, Yasuhiko| Fujiwara, Masachika| Nakatsukasa, Harushige| Kobayashi, Michio| Watanabe, Akiharu|
Abstract <p>Cathepsin B, H and L activities in small amounts of rat tissue homogenates corresponding to 10 micrograms protein were determined with 7-amino-4-methyl-coumarin conjugates as substrates. A new procedure for serum cathepsin H activity was also developed. High cathepsin B and H activities were found in kidney, spleen and liver. Liver cathepsin B, H and L activities in D-galactosamine-injured rats were decreased concomitantly with an increase in serum cathepsin H activity.</p>
Keywords cathepsin B H and L serum cathepsin H liver injury.
Amo Type Article
Published Date 1986-02
Publication Title Acta Medica Okayama
Volume volume40
Issue issue1
Publisher Okayama University Medical School
Start Page 27
End Page 32
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3962728
Web of Science KeyUT A1986A190200004
JaLCDOI 10.18926/AMO/31933
FullText URL fulltext.pdf
Author Kobayashi, Michio| Nakatsukasa, Harushige| Watanabe, Akiharu| Yamauchi, Yasuhiko| Fujiwara, Masachika| Hashimoto, Makoto| Watanabe, Masatomo| Higashi, Toshihiro| Nagashima, Hideo|
Abstract <p>&lt;p&gt;Type V collagen-degrading enzyme activity was detected as a metalloprotease acting at neutral pH in the human liver. Type V collagen extracted from human placenta and labeled with [1-14C] acetic anhydride was used as the substrate in the assay. Four major degradation products with relatively high molecular weights were observed upon polyacrylamide gel electrophoresis of the incubation mixture of type V collagen and liver homogenate. The significance of the measurement of this enzyme activity was discussed in relation to the clarification of the mechanism of liver fibrosis.&lt;/p&gt;</p>
Keywords type V collagen-degrading enzyme activity human liver liver fibrosis collagen degradation products
Amo Type Article
Published Date 1986-06
Publication Title Acta Medica Okayama
Volume volume40
Issue issue3
Publisher Okayama University Medical School
Start Page 179
End Page 182
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3017052
Web of Sience KeyUT A1986C914800007
JaLCDOI 10.18926/AMO/31584
FullText URL fulltext.pdf
Author Ling, Liu| Higashi, Toshihiro| Tsuchida, Shigeki| Sato, Kiyomi| Tsuji, Takao|
Abstract <p>Reduced indocyanine green (ICG) uptake is one of the functional changes of human hepatocellular carcinoma (HCC). To clarify the mechanisms of loss of ICG uptake, and determine which subunit of glutathione-S-transferase (GST), alpha or pi, plays a role in ICG transport in hepatocytes, an experimental HCC model was developed that used nodules induced by 2-acetylamino-fluorene (2-AAF) administration. Many of the ICG stained nodules, which consisted of benign and borderline lesions, were GST-alpha positive. However, the percentage of GST-alpha positive cells tended to decrease according to the disappearance of ICG staining in the process of hepatocarcinogenesis. HCCs unstained by ICG were also GST-alpha negative. GST-pi, not detected in normal rat hepatocytes, appeared in an earlier stage of hepatocarcinogenesis before the disappearance of GST-alpha, and was not observed in HCCs. No significant relationship between ICG staining and GST-pi was recognized. These results suggest that GST-alpha synthesis is disturbed in the process of hepatocarcinogenesis and results in loss of ICG uptake in HCCs, and also indicate that GST-pi may be useful for early diagnosis of preneoplastic hepatocytes showing no roles in ICG transport.</p>
Keywords hepatocarcinogenesis indocyanine green glutathione-S-transferase-? glutathione-S-transferase-?
Amo Type Article
Published Date 1993-10
Publication Title Acta Medica Okayama
Volume volume47
Issue issue5
Publisher Okayama University Medical School
Start Page 293
End Page 298
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7505994
Web of Science KeyUT A1993ME47100001
JaLCDOI 10.18926/AMO/31563
FullText URL fulltext.pdf
Author Nouso, Kazuhiro| Battula, Narayama| Thorgeirsson, Snorri S| Higashi, Toshihiro| Tsuji, Takao|
Abstract <p>We expressed mouse cytochrome P1-450 and P3-450 using recombinant vaccinia virus gene expression system in HeLa cells that were devoid of significant basal levels of P-450. HeLa cells were infected with the recombinant vaccinia virus containing either mouse cytochrome P1-450 or P3-450 cDNA, and the cell lysates were analyzed for the kinetics of P-450 enzyme activity and protein expression at the same time. 7-Ethoxycoumarin O-deethylase and ethoxyresorufin O-deethylase activities were measured as an expression of the cytochrome P-450 enzyme activities. Both cell lines began to express these enzyme activities as early as 12h after infection. The activities increased linearly up to the 24 h time point, and were kept for 36 h. Western immunoblot analysis showed that these cytochrome P-450 proteins were detected at 16 h and reached maximum quantity at 24 h after infection. These data showed a good correlation between cytochrome P-450 enzyme activity and protein concentration throughout the process of P-450 gene expression by vaccinia virus vector, suggesting a complete formation of cytochrome P-450 holoenzyme from the early stage of the protein expression.</p>
Keywords cytochrome P-450 vaccinia virus kinetics
Amo Type Article
Published Date 1993-12
Publication Title Acta Medica Okayama
Volume volume47
Issue issue6
Publisher Okayama University Medical School
Start Page 377
End Page 382
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8128911
Web of Sience KeyUT A1993MP00700004
JaLCDOI 10.18926/AMO/31549
FullText URL fulltext.pdf
Author Hino, Naoki| Higashi, Toshihiro| Ueki, Tooru| Nakatsukasa, Harushige| Ooguchi, Souhei| Ashida, Kouzou| Fujio, Kouzou| Takaki, Akinobu| Yonei, Taiji| Hasui, Toshimi| Tsuji, Hideyuki| Tsuji, Takao|
Abstract <p>We report here a case of hepatocellular carcinoma (HCC) with multiple lung metastases, which were disappeared by treatment of OK-432. The patient was a 65-year-old man and was diagnosed in 1986 with a small (17 x 11 mm) HCC in the anterior-superior segment of the liver. A part of the right hepatic lobe including the tumor was surgically removed, and transarterial injections of adriamycin (10 mg/week) and subcutaneous injections of OK-432 (10 KE/week) were given. Two and a half years later, recurrence of HCC in the liver and its invasion to vena cava inferior (IVC) were found. OK-432 administration was then stopped and percutaneous ethanol injection therapy (PEIT) was performed 10 times. Six months later, the PEIT was effective and the liver tumor with IVC invasion diminished. However, multiple lung metastases were visible on roentgenograms of the chest, and serum alphafetoprotein (AFP) concentration increased to 50,000 ng/ml. The OK-432 treatment resumed. After 6 months of OK-432 treatment, the multiple lung metastases were disappeared and the serum AFP level decreased to 100 ng/ml. At present, the patient is surviving without any sign of recurrence in either the liver or the lung. The clinical course of this case suggests that OK-432 might have effectively treated lung metastases of HCC, although the exact mechanisms are at present unclear.</p>
Keywords hepatocellular carcinoma OK-432 pulmonary metastasis
Amo Type Article
Published Date 1993-08
Publication Title Acta Medica Okayama
Volume volume47
Issue issue4
Publisher Okayama University Medical School
Start Page 289
End Page 292
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7692705
Web of Science KeyUT A1993LV73800011
JaLCDOI 10.18926/AMO/31540
FullText URL fulltext.pdf
Author Fujiwara, Masachika| Watanabe, Akiharu| Yamauchi, Yasuhiko| Hashimoto, Makoto| Nakatsukasa, Harushige| Kobayashi, Michio| Higashi, Toshihiro| Nagashima, Hideo|
Abstract <p>The clinicopathological findings of cerebral edema were investigated in patients with acute hepatic failure autopsied at Okayama University Hospital between 1970 and 1980 retrospectively. Nine (64%) of 14 hepatic failure cases were found to have cerebral edema during a post-mortem examination of the brain. Clinical features of the patients with cerebral edema were not significantly different from those of the patients without cerebral edema. However, general convulsions were observed more frequently in patients later found to have cerebral edema. Moreover, the length of time from deep coma to death was much shorter in the brain edema cases with cerebral herniation than without herniation.</p>
Keywords acute hepatic failure fulminant hepatitis cerebral edema neurological abnormalities
Amo Type Article
Published Date 1985-02
Publication Title Acta Medica Okayama
Volume volume39
Issue issue1
Publisher Okayama University Medical School
Start Page 72
End Page 75
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3984783
Web of Science KeyUT A1985ACS5600009
JaLCDOI 10.18926/AMO/31264
FullText URL fulltext.pdf
Author Obata, Takahiro| Watanabe, Akiharu| Hayashi, Shosuku| Takei, Nobuyuki| Sakata, Tatsuro| Shiota, Tetsuya| Higashi, Toshihiro| Itoshima, Tatsuya| Nagashima, Hideo|
Abstract <p>Cholestasis with htperbilirubinemia was induced in female, but not male, Sprague-Dawley rats by daily treatment with phalloidin for 7 days. Increases in serum direct bilirubin level and alkaline phosphatase (Al-Pase) activity were observed concomitantpy with diminished bile flow and a decreased output of bile acid and cholesterol. Kidht microscope findings of the liver revealed proliferated bile ductules and enhanced mitosis of hepatocytes.</p>
Keywords phalloidin cholestasis hyperbilirubinemia mitosis bile ductule.
Amo Type Article
Published Date 1981-12
Publication Title Acta Medica Okayama
Volume volume35
Issue issue6
Publisher Okayama University Medical School
Start Page 411
End Page 415
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6459712
Web of Science KeyUT A1981MV25300004
JaLCDOI 10.18926/AMO/31261
FullText URL fulltext.pdf
Author Obata, Takahiro| Watanabe, Akiharu| Hayashi, Shosaku| Takei, Nobuyuki| Sakata, Tatsuro| Shiota, Tetsuya| Higashi, Toshihiro| Itoshima, Tatsuya| Nagashima, Hideo|
Abstract <p>Cholestasis with hyperbilirubinemia was induced in female, but not male, Sprague-Dawley rats by daily treatment with phalloidin for 7 days. Increases in serum direct bilirubin level and alkaline phosphatase (Al-Pase) activity were observed concomitantly with diminished bile flow and a decreased output of bile acid and cholesterol. Light microscope findings of the liver revealed proliferated bile ductules and enhanced mitosis of hepatocytes.</p>
Keywords phalloidin cholestasis hyperbilirubinemia mitosis bile ductule.
Amo Type Article
Published Date 1981-12
Publication Title Acta Medica Okayama
Volume volume35
Issue issue6
Publisher Okayama University Medical School
Start Page 411
End Page 415
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6459712
Web of Sience KeyUT A1981MV25300004
Author Watanabe, Akiharu| Higashi, Toshihiro| Nagashima, Hideo|
Published Date 1978-12
Publication Title Acta Medica Okayama
Volume volume32
Issue issue6
Content Type Journal Article
JaLCDOI 10.18926/AMO/31032
FullText URL fulltext.pdf
Author Higashi, Toshihiro| Tobe, Kazuo| Asano, Ken-ichiro| Ikeda, Hiroshi| Ohsawa, Toshiya| Iwasaki, Yoshiaki| Nouso, Kazuhiro| Shinji, Noriyuki| Morimoto, Yohichi| Satoh, Yasumasa| Andoh, Masaharu| Araki, Yasuyuki| Tomita, Osamu| Morishita, Hirofumi| Kita, Keiji| Tsuchiya, Takahiro| Morichika, Shigeru| Tanabe, Takahiro| Nagashima, Hideo| Tsuji, Takao|
Abstract <p>The ultrasonographic characteristics of hepatocellular carcinomas (HCC) were investigated. Four typical features of HCCs, "mosaic internal echo pattern", "halo", "lateral shadow" and "posterior echo enhancement", were not recognized in minute HCCs smaller than 2 cm in diameter. These characteristics developed as the tumors grew. Only hypoechoic space-occupying lesions can be considered as small HCCs. In differentiating small HCCs from hypoechoic non-malignant space-occupying lesions in the cirrhotic liver, the ratios of short to long dimensions of the lesions seemed to be important since the ratios of HCCs were significantly larger than those of non-malignant lesions. The fact that 3 hyperechoic small HCCs could not be diagnosed even by celiac arteriography has suggested to us that ultrasonically guided biopsies should be performed in order to differentiate from small hemangiomas. Serum alpha-fetoprotein (AFP) levels of 1/3 of the patients with HCCs were below 100 ng/ml, indicating that it is impossible to detect small HCCs only by measuring serum AFP.</p>
Keywords ultrasonography hepatocellular carcinoma alpha-fetoprotein
Amo Type Article
Published Date 1988-06
Publication Title Acta Medica Okayama
Volume volume42
Issue issue3
Publisher Okayama University Medical School
Start Page 151
End Page 157
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2456671
Web of Science KeyUT A1988P034000005
JaLCDOI 10.18926/AMO/30894
FullText URL fulltext.pdf
Author Ohsawa, Toshiya| Higashi, Toshihiro| Tsuji, Takao|
Abstract <p>The biochemical characteristics of cathepsin B secreted from cultured human liver cancer cells were examined. The enzyme activity of culture medium against a synthetic substrate, N-carbobenzoxy-L-arginyl-L-arginine-4-methyl-coumaryl-7-amide, was dependent on the addition of cysteine, and the optimal pH was found to be 6.0. No activity was observed when the enzyme source was fresh medium not used for culture. These results suggest that the enzyme released from liver cancer cells is the thiol-protease cathepsin B. The molecular weight of the enzyme with 90% of the total activity was 40,000. Two cathepsin B molecules were found in liver tissue from patients with hepatocellular carcinoma (HCC); one was equivalent in size to the secreted enzyme, and a smaller one was the same as normal liver cathepsin B (27,000), which was also obtained from HCC-bearing cirrhotic liver. These results demonstrate that two molecules of cathepsin B are synthesized in liver cancer, and that the larger one is released into the surrounding tissue.</p>
Keywords cathepsin B cathepsin B secretion cultured human liver cancer
Amo Type Article
Published Date 1989-02
Publication Title Acta Medica Okayama
Volume volume43
Issue issue1
Publisher Okayama University Medical School
Start Page 9
End Page 15
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2718772
Web of Science KeyUT A1989T938500002
JaLCDOI 10.18926/AMO/30852
FullText URL fulltext.pdf
Author Itoshima, Tatsuya| Fedail, Sulieman S.| Suliman, Ikhlas| Ali, Abbas K.| Higashi, Toshihiro| Tsuji, Takao|
Abstract <p>Markers of hepatitis A and B virus were tested in 88 adult Sudanese subjects in Khartoum, Sudan. The subjects consisted of 25 control hospitalized patients, 21 volunteer blood donors, 23 patients with hepatosplenic schistosomiasis, 13 patients with liver cirrhosis and 6 patients with hepatocellular carcinoma (HCC). Antibody to hepatitis A virus was detected in 96% of the total. Hepatitis B surface antigen (HBsAg) was positive in 4, 24, 22, 31, and 67% of the subject groups, respectively. Antibody against hepatitis B core antigen (HBcAb) of undiluted serum was positive in 60, 57, 65, 77 and 83%, and there was no difference in incidence among the groups. It was positive in 200X diluted serum in 4, 24, 17, 23 and 60%. HBsAg and HBcAb (200X) were detected more often in HCC patients than in the control subjects (p less than 0.01). Hepatitis B virus is an important factor in the etiology of HCC in the Sudan.</p>
Keywords hepatitis B virus markers liver cirrhosis hepatocellular carcinoma schistosomiasis Sudan
Amo Type Article
Published Date 1989-08
Publication Title Acta Medica Okayama
Volume volume43
Issue issue4
Publisher Okayama University Medical School
Start Page 241
End Page 244
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2552752
Web of Science KeyUT A1989AP79100006
JaLCDOI 10.18926/AMO/30669
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Higashi, Toshihiro| Hayashi, Shosaku| Obata, Takahiro| Sakata, Tatsuro| Takei, Nobuyuki| Shiota, Tetsuya| Nagashima, Hideo|
Abstract <p>The effects of insulin and glucagon administration on serum amino acid levels were investigated in patients with severe liver disease, since simultaneous injection of pancreatic hormones has been recently introduced as a therapeutic approach. The changes in serum amino acid concentrations, as observed 3 h after ceasing a 3 h infusion of insulin and glucagon in 500 ml glucose solution, were an elevation of serum branched chain amino acid (BACA) levels and of the molar ratio of BCAA/aromatic amino acid (AAA) levels in patients with liver cirrhosis. Similar increases of serum BCAA levels during the infusion were also observed in patients with fulminant hepatitis. The results suggest that insulin-glucagon therapy for severe liver disease has no harmful side effects at least with respect to alterations in the serum aminogram.</p>
Keywords glucagon insulin amino acid liver disease
Amo Type Article
Published Date 1982-12
Publication Title Acta Medica Okayama
Volume volume36
Issue issue6
Publisher Okayama University Medical School
Start Page 441
End Page 446
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6760674
Web of Science KeyUT A1982PW07500002
JaLCDOI 10.18926/AMO/30498
FullText URL fulltext.pdf
Author Kimura, Yoshihiro| Higashi, Toshihiro| Kuwahara, Naoaki| Nouso, Kazuhiro| Ohguchi, Shouhei| Hino, Naoki| Tanimizu, Masato| Nakatsukasa, Harushige| Tobe, Kazuo| Tsuji, Takao|
Abstract <p>To diagnose hepatocellular carcinoma (HCC) functionally and immediately, we examined the usefulness of indocyanine green (ICG) injection during ultrasound-guided liver biopsy. Liver specimens were obtained after intravenous ICG injection by ultrasound-guided biopsy from 251 space-occupying lesions (SOL) in 136 patients. The tissues were immediately examined for ICG uptake using an infrared Vidicon camera and were also subjected to histopathological examinations. Of the 112 ICG-negative biopsy specimens, 105 were histologically diagnosed as HCC, 6 as dysplastic nodules (DN) and 1 as a regenerative nodule (RN). Of the 139 ICG-positive specimens, 18 were diagnosed as HCC, 1 as DN and 120 as RN. The sensitivity of the absence of ICG uptake (SEAIU), the specificity of the absence of ICG uptake (SPAIU), and the positive predictive value of the absence of ICG uptake (PPAIU) for the diagnosis of HCC were 85.3%, 94.5% and 93.8%, respectively. Of the 251 SOLs, 184 were less than 2 cm. SEAIU, SPAIU and PPAIU for the diagnosis of these small HCC were 85.3%, 94.5% and 91.4%, respectively. These results support the reliability of ICG injection during ultrasound-guided liver biopsy to diagnose even small HCC.</p>
Keywords indocyanine green ultrasound liver biopsy diagnosis hepatocellular carcinoma
Amo Type Article
Published Date 1996-10
Publication Title Acta Medica Okayama
Volume volume50
Issue issue5
Publisher Okayama University Medical School
Start Page 255
End Page 259
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8914678
Web of Science KeyUT A1996VQ20600004