JaLCDOI 10.18926/AMO/30872
FullText URL fulltext.pdf
Author Ichikawa, Yoshiko|
Abstract <p>Murine adrenal tumor cells (Y-1 clone) were stimulated by adrenocorticotropic hormone (ACTH) and cyclic adenosine 3',5'-monophosphate (cyclic AMP) to produce steroid hormone (delta 4, 3-keto steroids). The steroids were secreted into the medium immediately after synthesis. The optimum concentrations of ACTH and cyclic AMP for stimulation of steroid production were 10(-2) U/ml and 1.0 mM, respectively. In serum-free medium, ACTH and cyclic AMP stimulated steroidogenesis in Y-1 cells, but the amount of steroid hormone in the culture medium was low. However, a high level of steroid production was maintained with medium containing 10 mg/ml bovine serum albumin (BSA). In culture medium containing a higher concentration of BSA, Y-1 cells did not become spherical as is usually the case when steroid production is stimulated by ACTH or cyclic AMP. The morphological changes did not always correlate with steroid secretion by Y-1 cells.</p>
Keywords Y-1 clone steroid hormone ACTH cyclic AMP
Amo Type Article
Published Date 1989-04
Publication Title Acta Medica Okayama
Volume volume43
Issue issue2
Publisher Okayama University Medical School
Start Page 97
End Page 103
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2543184
Web of Science KeyUT A1989U578500004