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ID 13634
Eprint ID
13634
FullText URL
Thumnail 113_33.pdf 3.77 MB
Title Alternative
Establishment and characterization of cell line from ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma
Author
Kakehashi, Chikako
Mori, Masaharu
Kawabata, Teruyuki
Okada, Shigeru
Abstract
The characteristics of ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma by intraperitoneal administration and the effects of hormones on the tumor have been studied in vivo. However, to our knowledge there have been no reports on the characteristics of the tumor cells. In this study, we established a tumor cell line from Fe-NTA induced rat renal tumour and investigated tumor cell characteristics. The tumor cell line RKFe was established from Fe-NTA induced rat renal tumor by collagenase digestion. RKFe showed epithelial morphology in culture, and had microvilli and basement membranes under electron microscopy. RKFe cells acquired tolerance to cytotoxicity of Fe-NTA. They also grew in soft agar culture and formed tumors by the inoculation of cells into nude mice. Histologically, transplanted tumors showed poorly defferentiated adenocarcinoma with several tubular structures. A subclone of RKFe, named RKFe-C6, showed metastasis to lungs. The RKFe cell line may be useful for studying the characteristics of Fe-NTA induced rat tumor cells.
Keywords
Fe-NTA
腎癌
培養細胞株の樹立
Note
原著
Published Date
2001-04-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume113
Issue
issue1
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
33
End Page
39
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/113/1/113_1_33/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma