JaLCDOI 10.18926/AMO/32168
FullText URL fulltext.pdf
Author Liu, Jiankang| Kabuto, Hideaki| Hiramatsu, Midori| Mori, Akitane|
Abstract <p>Guillingji powder, a complex prescription of traditional Chinese medicines, has been pharmacologically shown to be a possible anti-aging agent, and also used for the treatment of male hypogonadism and female dysgenesis. Since the levels of numerous brain monoamines and their metabolites such as norepinephrine (NE), dopamine (DA), dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), serotonin (5-HT), and 5-hydroxyindole acetic acid (5-HIAA) have been correlated with aging, we examined the effects of Guilingji powder and its extract on such monoamines and metabolites in the cerebrum and the cerebellum of mice using HPLC-ECD. The results showed that dietary intake of Guilingji powder had the effect of increasing the NE level and the DOPAC/DA ratio, and decreasing the 5-HT level and 5-HT/NE ratio in the cerebrum. In the cerebellum, the powder did not produce any changes in the levels of the compounds investigated. The extract of Guilingji, on the other hand, not only increased the NE level and the DOPAC/DA ratio but also the 5-HIAA/5-HT ratio in the cerebrum. However, the extract did not decrease the 5-HT in the cerebrum, although it decreased the 5-HT/NE. Unlike the powder, the extract had an effect in the cerebellum, which increased the DOPAC/DA ratio. These results suggest that Guilingji may have the action of modulating the levels and the metabolism of monoamines.</p>
Keywords Guilingji monoamines norepinephrine serotonin metabolism
Amo Type Article
Published Date 1991-08
Publication Title Acta Medica Okayama
Volume volume45
Issue issue4
Publisher Okayama University Medical School
Start Page 217
End Page 222
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1962529
Web of Science KeyUT A1991GD78000003
JaLCDOI 10.18926/AMO/31102
FullText URL fulltext.pdf
Author Kabuto, Hideaki| Yokoi, Isao| Endo, Atsushi| Takei, Mineo| Kurimoto, Tadashi| Mori, Akitane|
Abstract <p>Serotonin (5-HT) is thought to play an important role in the seizures of El mice because the seizure threshold of El mice correlates with the 5-HT concentration in the central nervous system. In this study, the anticonvulsant effect of a 5-HT reuptake blocker, citalopram, was evaluated behaviorally and biochemically. El mouse convulsions were inhibited by chronic administration of citalopram (80 mg/kg/day, p.o. for 2 weeks), but were not inhibited by acute administration of citalopram (80 mg/kg, i.p., 2 h after single injection). Both chronic and acute administration of citalopram decreased the concentration of 5-hydroxyindolacetic acid in the brain, whereas the concentration of 5-HT was not changed by treatment with citalopram. Tryptophan hydroxylase activity was not different between the citalopram and control groups, although the monoamine oxydase-A activity was lowered by chronic administration of citalopram. These findings suggest that both acute and chronic administration of citalopram depresses the 5-HT turnover rate, however chronic administration is necessary to inhibit El mouse convulsions.</p>
Keywords citalopram serotonin MAO-A Trp-OHase EL mouse
Amo Type Article
Published Date 1994-12
Publication Title Acta Medica Okayama
Volume volume48
Issue issue6
Publisher Okayama University Medical School
Start Page 311
End Page 316
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7535969
Web of Science KeyUT A1994PZ34600005