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ID 13218
Eprint ID
13218
FullText URL
Thumnail 119_247.pdf 400 KB
Title Alternative
Impaired coronary capillary hemodynamics with decreased glycocalyx thickness and irregular inner wall remodeling in right ventricle of pulmonary hypertensive rats
Author
Kajiya, Masahito
Abstract
We hypothesized that coronary capillary function is impaired with a decreased glycocalyx layer and uneven inner wall remodeling in right ventricles that are hypertrophied due to pulmonary hypertension (PH). Five-week-old male Sprague-Dawley rats were divided into 2 groups (PH: n=25, control: n=27). In the PH group, Monocrotaline (60 mg/kg SC) was administrated at 5 weeks. Coronary capillary hemodynamics was visualized in vivo at 8 weeks of age using our high-resolution intravital videomicrosopy, and RBC velocities in RV capillaries were evaluated, along with capillary diameters. Configuration of the capillary cast was assessed by a confocal laser scanning microscope. The thickness of the glycocalyx on the capillary endothelial surface was evaluated with Alcian blue 8GX by electron microscopy. RV systolic pressure increased in PH (by 142%, p<0.01). The diameters of capillaries were uneven in PH (coefficient of variation of diameters along capillary trees; 24±8% vs. 11±3%, p<0.05). The thickness of the glycocalyx in PH was less than half that of control (0.20±0.05 vs. 0.45±0.14 μm, p<0.05). RBC velocity was decreased in PH (820±110 vs. 1,100±230 μm/sec, p<0.05). The capillary flow reduction in PH may be closely related to the changes in the glycocalyx and vessel irregularity, resulting in insufficient oxygen supply to the heart.
Keywords
モノクロタリン (monocrotaline)
肺高血圧 (pulmonary hypertension)
冠毛細管 (coronary capillary)
グリコカリックス (glycocalyx)
リモデリング (remodeling)
Note
原著
Published Date
2008-01-04
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume119
Issue
issue3
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
247
End Page
251
ISSN
00301558
NCID
AN00032489
Content Type
Journal Article
Official Url
http://www.jstage.jst.go.jp/article/joma/119/3/119_247/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma