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ID 53654
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Author
Onishi, Manabu
Michiue, Hiroyuki Kakenhi
Fujii, Kentaro
Ishida, Joji
Shimazu, Yosuke
Chiocca, E Antonio
Kaur, Balveen
Abstract
Cilengitide (EMD121974), an inhibitor of the adhesive function of integrins, demonstrated preclinical efficacy against malignant glioma. It is speculated that cilengitide can inhibit tumor growth, invasion, and angiogenesis. However, the effects of cilengitide on these processes have not been sufficiently examined. In this study, we investigated the anti-glioma effect of cilengitide using DNA microarray analysis. U87ΔEGFR cells (human malignant glioma cell line) were used for this experiment. The cells were harvested after 16 h of cilengitide treatment, and mRNA was extracted. Gene expression and pathway analyses were performed using a DNA microarray (CodeLink™Human Whole Genome Bioarray). The expression of 265 genes was changed with cilengitide treatment. The expression of 214 genes was up-regulated by more than 4-fold and the expression of 51 genes was down-regulated by more than 4-fold compared to the controls. In pathway analysis, "apoptotic cleavage of cellular proteins" and "TNF receptor signaling pathway" were over-represented. Apoptotic-associated genes such as caspase 8 were up-regulated. Gene expression profiling revealed more detailed mechanism of the anti-glioma effect of cilengitide. Genes associated with apoptosis were over-represented following cilengitide treatment.
Keywords
Glioma
Integrin
Cilengitide
Gene expression profiling
Apoptosis
Note
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Published Date
2013-04-15
Publication Title
SpringerPlus
Volume
volume2
Publisher
Springer International Publishing
Start Page
160
ISSN
2193-1801
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1186/2193-1801-2-160
language
英語
Copyright Holders
© 2013 Onishi et al.; licensee Springer.
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Refereed
True
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