JaLCDOI 10.18926/AMO/31744
FullText URL fulltext.pdf
Author Hayashi, Hisatomo| Tsuda, Takashi| Tsurumi, Naokazu| Takai, Yutaka| Maeda, Masanori| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract <p>A significant amount of anticoagulant substance was released along with histamine, when human lung mast cells were stimulated with anti-IgE and Ca-ionophore A23187. Its activity was lost by heparinase, not by chondroitin-ABC lyase or chondroitin-AC lyase, and also inhibited by Polybrene, suggesting it would be heparin.</p>
Keywords human lung mast cells heparin anticoagulant activity anti-IgE Ca-ionophore
Amo Type Article
Published Date 1987-04
Publication Title Acta Medica Okayama
Volume volume41
Issue issue2
Publisher Okayama University Medical School
Start Page 85
End Page 87
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2438904
Web of Science KeyUT A1987H040200006
JaLCDOI 10.18926/AMO/31572
FullText URL fulltext.pdf
Author Tanabe, Kozo| Takahashi, Kiyoshi| Maeda, Masanori| Kimura, Ikuro|
Abstract <p>To confirm the formation of Charcot-Leyden crystals (CLC) in basophils, we observed basophils in sputum and peripheral blood. Sealed slide and suspension culture methods were used to observe the process of CLC formation in peripheral blood basophils and eosinophils under electron microscopy. CLC formation was observed in basophils and eosinophils, and was found to be augmented by sealed slide method. A temperature of 4 degrees C was better than 37 degrees C for promoting the formation of crystals. There was no correlation between the degranulation of these cells and the formation of CLC after stimulation with anti-IgE or anti-IgG antibodies. CLC were initially detected in the cytoplasmic granules of basophils where they continued to enlarge. No CLC were identified in mast cells under any conditions studied. These findings confirm that CLC in sputum are not exclusive to eosinophils and that CLC appear to be present in basophil-rich sites under the cell damage.</p>
Keywords Charcot-Leyden crystals basphils electron microscopy
Amo Type Article
Published Date 1993-04
Publication Title Acta Medica Okayama
Volume volume47
Issue issue2
Publisher Okayama University Medical School
Start Page 85
End Page 90
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8506754
Web of Sience KeyUT A1993LA45200003
JaLCDOI 10.18926/AMO/31498
FullText URL fulltext.pdf
Author Tanizaki, Yoshiro| Komagoe, Haruki| Morinaga, Hiroshi| Ohtani, Jun| Nakagawa, Saburo| Maeda, Masanori| Kitani, Hikaru| Takahashi, Kiyoshi| Kimura, Ikuro|
Abstract <p>The inhibitory effect of nicardipine, a calcium antagonist, on the antigen- and anti-IgE-induced histamine release from basophilic leucocytes of patients with bronchial asthma was examined. The agent significantly inhibited both antigen-stimulated and anti-IgE-induced histamine release from basophils (the maximum percent inhibition was 57.8 +/- 7.2% and 56.0 +/- 8.8%, respectively). Pre-incubation of basophils with nicardipine for periods of up to 120 min did not alter the inhibitory effect. These results suggest that nicardipine modifies the histamine release from basophils which closely participate in an attack of bronchial asthma.</p>
Keywords histamine release basophils antigen anti-IgE Ca<sup>2+</sup> antagonist
Amo Type Article
Published Date 1985-08
Publication Title Acta Medica Okayama
Volume volume39
Issue issue4
Publisher Okayama University Medical School
Start Page 247
End Page 251
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2413722
Web of Science KeyUT A1985APN0700001