JaLCDOI 10.18926/AMO/55305
FullText URL 71_4_291.pdf
Author Naganuma, Atsushi| Hoshino, Takashi| Suzuki, Yuhei| Uehara, Daisuke| Kudo, Tomohiro| Ishihara, Hiroshi| Sato, Ken| Kakizaki, Satoru| Yamada, Masanobu| Takagi, Hitoshi|
Abstract The effect of skeletal muscle mass (SMM) on the outcomes of sorafenib treatment for hepatocellular carcinoma (HCC) has not been established. We measured the SMM in HCC patients treated with sorafenib, evaluated the patients’ survival, and evaluated the association between skeletal muscle depletion and sorafenib treatment. Of the 97 HCC patients treated with sorafenib at our institution in the period from July 2009 to February 2015, our study included 69 patients (51 males, 18 females) who had received sorafenib for ≥ 8 weeks and whose follow-up data were available. SMM was calculated from computed tomography images at the mid-L3 level (cm2) and normalized to height (m2) to yield the L3 skeletal muscle index (L3-SMI, cm2/m2). The median L3-SMI value was higher in the males (43 cm2/m2) compared to the females (36 cm2/m2). In the males only, the multivariate Cox regression identified an L3-SMI <43 cm2/m2 as independently associated with higher mortality compared to an L3-SMI ≥43 cm2/m2 (hazard ratio 2.315, 95% confidence interval: 1.125-4.765, p=0.023). Skeletal muscle depletion is a factor predicting poor prognosis for male patients with advanced HCC treated with sorafenib.
Keywords skeletal muscle depletion hepatocellular carcinoma sorafenib L3 skeletal muscle index prognostic factor
Amo Type Original Article
Published Date 2017-08
Publication Title Acta Medica Okayama
Volume volume71
Issue issue4
Publisher Okayama University Medical School
Start Page 291
End Page 299
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28824184
JaLCDOI 10.18926/AMO/32281
FullText URL fulltext.pdf
Author Nozaki, Akito| Naganuma, Atsushi| Nakamura, Takashi| Tanaka, Katsuaki| Sekihara, Hisahiko| Kato, Nobuyuki|
Abstract <p>We have developed a reliable internally controlled RT-nested PCR method for the detection of hepatitis C virus (HCV) RNA using in vitro synthesized Renilla luciferase (Rluc) RNA as an internal control. Using this method, the 5'-noncoding region of HCV RNA (144 nucleotides) and Rluc RNA (276 nucleotides) were efficiently amplified in a single tube, and the sensitivity and specificity of this method were comparable to standard RT-nested PCR. This method was successfully performed on RNA specimens obtained from in vitro HCV-infected human hepatocyte PH5CH8 cells, which support HCV replication. In addition, we demonstrated that this method was useful for the evaluation of antiviral reagents by confirming the anti-HCV activity of bovine lactoferrin, which we previously found to be a new inhibitor of HCV infection. Therefore, this method may be useful for the studies of not only HCV but also of other viruses.</p>
Keywords Hepatitis C virus Reverse transcriptionnested PCR (RT-nested PCR) internal control
Amo Type Article
Published Date 2000-12
Publication Title Acta Medica Okayama
Volume volume54
Issue issue6
Publisher Okayama University Medical School
Start Page 253
End Page 257
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11132918
Web of Science KeyUT 000166042900003
JaLCDOI 10.18926/AMO/31716
FullText URL fulltext.pdf
Author Alam, Shahjalal S.| Nakamura, Takashi| Naganuma, Atsushi| Nozaki, Akito| Nouso, Kazuhiro| Shimomura, Hiroyuki| Kato, Nobuyuki|
Abstract <p>We have shown that highly proofreading DNA polymerase is required for the polymerase chain reaction in the genetic analysis of hepatitis C virus (HCV). To clarify the status of HCV quasispecies in hepatic tissue using proofreading DNA polymerase, we performed a genetic analysis of the HCV core protein-encoding region in cancerous and noncancerous lesions derived from 4 patients with hepatocellular carcinoma. In contrast to the previously published data, we observed neither deletions nor stop codons in the analyzed region and no significant difference in the complexity of HCV quasispecies between cancerous and noncancerous lesions. This result suggests that the HCV core gene is never structurally defective in hepatic tissues, including cancerous lesions. However, in 3 of the patients, the consensus HCV species differed between cancerous and noncancerous lesions, suggesting that the predominant replicating HCV species differs between these 2 types of lesions. Moreover, during the course of the study, we obtained several interesting variants possessing a substitution at codon 9 of the core gene, whose substitution has been shown to induce the production of the F protein synthesized by a - 2/+1 ribosomal frameshift.</p>
Keywords hepatitis C virus core gene hepatocellular carcinoma quasispecies proofreading DNA polymerase
Amo Type Article
Published Date 2002-06
Publication Title Acta Medica Okayama
Volume volume56
Issue issue3
Publisher Okayama University Medical School
Start Page 141
End Page 147
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12108585
Web of Science KeyUT 000176521200004