JaLCDOI 10.18926/AMO/32449
FullText URL fulltext.pdf
Author Kawaguchi, Kenji| Itoshima, Tatsuya| Ukida, Minoru| Ito, Toshio| Kiyotoshi, Shozo| Ogawa, Hiromichi| Hattori, Shuzo| Kitadai, Masahiro| Mizutani, Shigeki| Nagashima, Hideo|
Abstract <p>The incidence of intraperitoneal adhesion after abdominal surgery was studied. Peritoneoscopy was performed in 933 patients with liver diseases over the 6 year 5 month period from March 1974 to July 1980. Of the patients, 352 (37.7%) had undergone an abdominal operation, and intraperitoneal adhesion was detected in 205 (58.2%) of these patients. The liver was not observable in 5 out of 61 patients with adhesions after upper abdominal operations. Whereas, the liver was clearly observable in patients with lower abdominal operations in spite of adhesions. Out of the 581 patients without any abdominal operations, 30 patients (5.2%) had adhesions in the abdominal cavity, and 6 of them had extensive adhesions that partially obscured the observation of liver surface. In all patients, peritoneoscopy was performed without complications by avoiding the surgical scar for puncture sites and ensuring a free air lumen before trocar puncture.</p>
Keywords peritoneoscopy liver adhesion abdominal operation
Amo Type Article
Published Date 1983-02
Publication Title Acta Medica Okayama
Volume volume37
Issue issue1
Publisher Okayama University Medical School
Start Page 67
End Page 72
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6221509
Web of Science KeyUT A1983QD83600008
JaLCDOI 10.18926/AMO/32448
FullText URL fulltext.pdf
Author Ogawa, Hiromichi| Itoshima, Tatsuya| Ito, Toshio| Kiyotoshi, Syozo| Kawaguchi, Kenji| Kitadai, Masahiro| Hattori, Syozo| Mizutani, SHigeki| Ukida, Minoru| Tobe, Kazuo| Nagashima, Hideo| Kobayashi, Toshinari|
Abstract <p>Absence of Kupffer cells in rat liver hyperplastic nodules induced by a chemical carcinogen was demonstrated by intravenous injection of indian ink. Hyperplastic nodules appeared 4 weeks after diethylnitrosamine (DEN) was administered, and the nodules continued growing and became eosinophilic hyperplastic nodules after 5 to 6 weeks. After intravenous injection of indian ink, hyperplastic nodules were observed as carbon-free white nodules, which were macroscopically distinguishable from the black surrounding tissue. As observed by light microscopy, Kupffer cells were absent in hyperplastic nodules in contrast to being present in the surrounding tissue. Scanning electron microscopy confirmed these findings and furthermore revealed that the sinusoidal endothelium of hyperplastic nodules had no fenestrae. Injection of indian ink is a useful method for delineation and enucleation of hyperplastic nodules in the study of morphological and chemical changes of nodules.</p>
Keywords liver hyperplastic nodule kupffer cell chemical carcinogenesis indian ink
Amo Type Article
Published Date 1983-02
Publication Title Acta Medica Okayama
Volume volume37
Issue issue1
Publisher Okayama University Medical School
Start Page 79
End Page 84
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6846054
Web of Science KeyUT A1983QD83600010
JaLCDOI 10.18926/AMO/32444
FullText URL fulltext.pdf
Author Shiota, Tetsuya| Watanabe, Akiharu| Mitani, Ken| Ito, Toshio| Tobe, Kazuo| Nagashima, Hideo|
Abstract <p>A patient with an unresectable hepatocellular carcinoma (HCC) who survived without active treatment 3 years and 8 months after histological diagnosis is described. The size of the liver, which was already quite huge at the time of diagnosis, changed little during the entire clinical observation. However, 2 months before death, his condition deteriorated rapidly following gastrointestinal bleeding due to the direct invasion of the stomach by HCC. A critical reason for the unusually long-term survival of the patient may stem from the facts that a well-differentiated and bile-producing HCC was extent in most encapsulated-tumor tissues and that liver cirrhosis was not present.</p>
Keywords hepatocellular carcinoma long-term survival well-differenciated type hepatobiliary scintigraphy
Amo Type Article
Published Date 1983-02
Publication Title Acta Medica Okayama
Volume volume37
Issue issue1
Publisher Okayama University Medical School
Start Page 73
End Page 78
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6189368
Web of Science KeyUT A1983QD83600009
JaLCDOI 10.18926/AMO/32442
FullText URL fulltext.pdf
Author Saito, Daiji| Yasuhara, Koichiro| Takeda, Kou| Hyodo, Tatsuo| Fujii, Akinobu| Uchida, Toshiaki| Abe, Yukihiro| Haraoka, Shoichi| Nagashima, Hideo|
Abstract <p>The effects of intravenous infusion of isoproterenol on stenosis resistance were studied in the anesthetized open-chest dog. The circumflex coronary artery (LCx) was isolated near its origin and an electromagnetic flow transducer was placed around the vessel for measuring coronary flow. A polyethylene catheter was inserted into the small branch of LCx for monitoring distal coronary pressure. LCx was constricted with a thick cotton string to a degree of obstruction that eliminated reactive hyperemia following a 20-second coronary occlusion. The coronary resistance across the stenotic segment (RL) was calculated as the pressure gradient across the stenosis divided by coronary flow. Isoproterenol was infused intravenously in a dose to keep the heart rate at a level 25-30% above the control with and without coronary constriction. For maintaining the ascending aortic pressure at the pre-isoproterenol level, the descending thoracic aorta was constricted with a tape. In the absence of coronary constriction, the vascular resistance of large coronary arteries was not affected by isoproterenol with a significant increase in coronary flow. In the presence of coronary stenosis, isoproterenol markedly increased RI regardless of additional aortic constriction. The magnitude of the increase in RL during aortic constriction varied directly with the percent increase in the pressure gradient across the coronary stenosis. Pacing-tachycardia essentially did not affect RL. These results suggest that isoproterenol increased the vascular resistance of the stenotic segment with fixed caliber.</p>
Keywords coronary cinstriction open-chest dog distal coronary pressure
Amo Type Article
Published Date 1983-02
Publication Title Acta Medica Okayama
Volume volume37
Issue issue1
Publisher Okayama University Medical School
Start Page 59
End Page 66
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6846053
Web of Science KeyUT A1983QD83600007
JaLCDOI 10.18926/AMO/32428
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Hayashi, Syosaku| Shiota, Tetsuya| Nagashima, Hideo|
Abstract <p>The blood levels of amino acids, ammonia and pancreatic hormones following the intragastric and intravenous administration of a branched-chain amino acid (BCAA)-enriched solution were comparatively investigated in control subjects and patients with liver cirrhosis. There was no essential difference in the time course of serum amino acid and blood ammonia levels between the intragastric and intravenous infusions. Elevation of serum insulin concentrations in cirrhotic patients was significant only immediately after the administration through the enteral route. However, plasma glucagon levels increased similarly when the BCAA-enriched solution was administered through either route. The results indicate that both enteral and intravenous infusions will have similar therapeutic effects on the impaired protein metabolism in cirrhotic patients with protein-calorie malnutrition.</p>
Keywords amino acied pancreatic hormone ammonia liver cirrhosis intragastric and intravenous infusion
Amo Type Brief Note
Published Date 1983-10
Publication Title Acta Medica Okayama
Volume volume37
Issue issue5
Publisher Okayama University Medical School
Start Page 441
End Page 445
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6417980
Web of Science KeyUT A1983RN98400008
JaLCDOI 10.18926/AMO/32425
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Ohashi, Yoshito| Nagashima, Hideo|
Abstract <p>Direct drain of the cystic duct and drain of the biliary tract through an internal fistula into the duodenum in a 81-year-old man were endoscopically diagnosed without an operation.</p>
Keywords bile duct anomaly cystic duct pancreatic duct internal fistula
Amo Type Article
Published Date 1983-10
Publication Title Acta Medica Okayama
Volume volume37
Issue issue5
Publisher Okayama University Medical School
Start Page 409
End Page 415
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6650223
Web of Science KeyUT A1983RN98400004
JaLCDOI 10.18926/AMO/32419
FullText URL fulltext.pdf
Author Saito, Daiji| Abe, Yukihiro| Takeda, Koh| Hyodo, Tazuo| Tani, Hideki| Ohnishi, Shigeru| Haraoka, Shoichi| Nagashima, Hideo|
Abstract <p>Stress-induced changes in the resistance due to coronary arterial stenosis of a fixed diameter and in the myocardial blood flow distal to the stenosis were investigated in the open-chest dog. Myocardial blood flow in the inner and outer third of the left ventricular wall was continuously measured with heated cross-thermocouples. The circumflex coronary artery was constricted with a thick string so that myocardial reactive hyperemia was nearly eliminated. Without constriction, a 15-second occlusion of the artery produced no significant changes in the resistance of large coronary arteries. On the contrary, in the presence of coronary constriction, a brief coronary occlusion caused a sustained decrease in distal coronary pressure and subendocardial myocardial flow during reactive hyperemia, while coronary flow returned quickly to the pre-occlusion level with significant reactive hyperemia of subepicardial flow. This change resulted in a long-lasting increase in the stenosis resistance. These results suggest that stenosis resistance changes dynamically, resulting in additional myocardial ischemia especially in the subendocardial myocardial layers.</p>
Keywords stenosis resistance coronary constriction transient myocardial ischemia myocardial flow
Amo Type Article
Published Date 1983-10
Publication Title Acta Medica Okayama
Volume volume37
Issue issue5
Publisher Okayama University Medical School
Start Page 423
End Page 429
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6650224
Web of Science KeyUT A1983RN98400006
JaLCDOI 10.18926/AMO/32412
FullText URL fulltext.pdf
Author Shiota, Tetsuya| Watanabe, Akiharu| Takei, Nobuyuki| Hayashi, Shosaku| Obata, Takahiro| Sakata, Tatsuro| Nagashima, Hideo|
Abstract <p>Blood ammonia levels in patients with various liver diseases were determined quantitatively by a simple and rapid method using the Amitest Meter System. The results were compared to those obtained by an enzymatic method and were well correlated. This simple Amitest is also useful in animal experiments, particularly when there is a need to determine blood ammonia levels serially. This paper test was evaluated as being accurate and reliable for clinical and experimental use.</p>
Keywords simple method blood ammonia hyperammonemia liver disease
Amo Type Brief Note
Published Date 1983-04
Publication Title Acta Medica Okayama
Volume volume37
Issue issue2
Publisher Okayama University Medical School
Start Page 159
End Page 162
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6869065
Web of Science KeyUT A1983QN63900009
JaLCDOI 10.18926/AMO/32406
FullText URL fulltext.pdf
Author Kobayashi, Michio| Watanabe, Akiharu| Nakatsukasa, Harushige| Fujiwara, Masachika| Shiota, Tetsuya| Takei, Nobuyuki| Sakata, Tatsuro| Yamauchi, Yasuhiko| Nagashima, Hideo|
Abstract <p>The prognoses of patients with alcoholic liver cirrhosis were compared between those who continued to drink and those who stopped. Clinical criteria were strictly set so as to control other variables affecting the prognoses. Four-year survival was significantly higher in the patients who stopped drinking than in those who continued to drink. Continued drinking worsens the prognosis of patients with alcoholic liver cirrhosis.</p>
Keywords alcoholic liver cirrhosis prognosis continued drinking stopped drinking alcohol consumption
Amo Type Brief Note
Published Date 1983-12
Publication Title Acta Medica Okayama
Volume volume37
Issue issue6
Publisher Okayama University Medical School
Start Page 525
End Page 527
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6666679
Web of Science KeyUT A1983RW62800010
JaLCDOI 10.18926/AMO/32401
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Nagashima, Hideo|
Abstract <p>Increased activities of liver glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (6PGD, EC 1.1.1.44) in the pentose phosphate cycle were accompanied with a depletion of reduced glutathione (GSH) following an intragastric administration of carbon tetrachloride (CCl4) to rats. Oxidized glutathione (GSSG) also decreased remarkably, keeping the GSSG: GSH ratio constant. No significant alteration of glutathione reductase (EC 1.6.4.2.), glutathione peroxidase (EC 1.11.1.9) and malic enzyme (EC 1.1.1.40) activities in the supernatant and gamma-glutamyl transpeptidase (gamma-GTP, EC 2.3.2.2) activity in the homogenate of the injured liver were observed. Furthermore, no marked difference in the GSH-synthesizing activity was found between control and CCl4-intoxicated liver. An intraperitoneal injection of GSH produced a significant increase in liver GSH content in control rats but not in CCl4-treated rats; G6PD activity was not affected. Intraperitoneal injections of diethylmaleate resulted in continuously diminished levels of liver GSH without any alteration of liver G6PD activity. In vitro disappearance of GSH added to the liver homogenate from CCl4-treated rats occurred enzymatically and could not be prevented by the addition of a NADPH-generating system. The results suggest that increased G6PD activity in CCl4-injured liver does not play an important role in the maintenance of glutathione in the reduced form and that the decreased GSH content in the injured liver might be caused by enhanced GSH catabolism not due to gamma-GTP.</p>
Keywords G6PD glutathione GSH GSSG CCL liver injury diethylmaleate
Amo Type Article
Published Date 1983-12
Publication Title Acta Medica Okayama
Volume volume37
Issue issue6
Publisher Okayama University Medical School
Start Page 463
End Page 470
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6666676
Web of Science KeyUT A1983RW62800002
JaLCDOI 10.18926/AMO/32398
FullText URL fulltext.pdf
Author Ito, Toshio| Yamamoto, Hiroshi| Itoshima, Tatsuya| Ukida, Minoru| Ogawa, Hiromichi| Kitadai, Masahiro| Hattori, Shuzo| Mizutani, Shigeki| Kita, Keiji| Tanaka, Ryoji| Yamauchi, Yasuhiko| Hashimoto, Keiji| Hayashi, Hidehiro| Aono, Kaname| Nagashima, Hideo|
Abstract <p>Computed tomographic arteriography (CTA) was performed in 30 patients with hepatocellular carcinoma (HCC). Detection of HCC by CTA was compared with that of conventional celiac or hepatic arteriography. CT scanning was performed immediately, 30 seconds and 1 min after an injection of 5 to 10 ml of contrast medium into the common or proper hepatic artery. Repeated infusions allowed whole liver sections to be visualized. HCC was localized in 28 of the 30 patients by conventional arteriography, with CTA detecting the masses in 27 of the 28 patients. CTA imaging presented the tumor mass in 1 of the 2 patients missed by arteriography. Conventional arteriography delineated the boundaries of HCC in 15 (50%) of the 30 patients. CTA clearly delineated the masses in 26 (87%) of the 30 patients including 11 patients in which the tumor borders were obscure by conventional arteriography. HCC lesions smaller than 1 cm in diameter were detected only by CTA in 6 (20%) of the patients. It was concluded that CTA is both useful and necessary in the demarcation of small HCC masses.</p>
Keywords computed tomographic arteriography hepatocellular carcinoma hepatic arteriography contrast enhancement
Amo Type Article
Published Date 1983-12
Publication Title Acta Medica Okayama
Volume volume37
Issue issue6
Publisher Okayama University Medical School
Start Page 503
End Page 510
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6320600
Web of Science KeyUT A1983RW62800006
JaLCDOI 10.18926/AMO/32395
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Takei, Nobuyuki| Hayashi, Syosaku| Nagashima, Hideo|
Abstract <p>Serum neutral amino acid levels in cirrhotic patients with abnormal oral glucose tolerance test patterns were not different from those of subjects without impaired carbohydrate metabolism. However, the characteristic features of serum aminograms in the patients, that is, increased levels of tyrosine, decreased levels of valine and leucine and the diminished ratio of branched chain amino acids to phenylalanine and tyrosine levels, were less pronounced in those treated with insulin. This finding is clinically important for evaluating the serum aminogram of cirrhotic patients under insulin therapy.</p>
Keywords amino acid cirrhotics carbohydrate metabolism insulin glucose tolerance test
Amo Type Brief Note
Published Date 1983-08
Publication Title Acta Medica Okayama
Volume volume37
Issue issue4
Publisher Okayama University Medical School
Start Page 381
End Page 384
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6353875
Web of Science KeyUT A1983RE57600011
JaLCDOI 10.18926/AMO/32393
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Shiota, Tetsuya| Okita, Misako| Nagashima, Hideo|
Abstract <p>A new nutritional product (SF-1008C) containing a high proportion of branched chain amino acids (BCAA) and low proportion of aromatic amino acids (AAA) and methionine was tested to see its effect on the impaired protein metabolism and abnormal nutritional state frequently observed in patients with advanced liver cirrhosis. A sharp increase in plasma BCAA levels and fall of AAA and methionine levels were found following the administration of an SF-1008C-supplemented diet to healthy controls and cirrhotic patients, which the BCAA levels increased only slightly following an isocaloric control diet. Blood ammonia levels increased within the normal range transiently following the diets. The SF-1008C-supplemented diet was given for 2 weeks to cirrhotic patients with histories of hepatic encephalopathy, who were taking a low-protein diet because of hyperammonemia. Serum prealbumin levels, nitrogen balance, molar ratio of plasma BCAA/phenylalanine and tyrosine, the number connection test and electroencephalograms improved during the period of the experimental diet. The results, therefore, indicate that a BCAA-supplemented diet is well tolerated by patients with advanced cirrhosis and useful for treatment of impaired protein metabolism. Furthermore, this product is beneficial in preventing hepatic encephalopathy in cirrhotics.</p>
Keywords branched chain amino acid aromatic amino acid nutritional product liver cirrhosis hepatic encephalopathy
Amo Type Article
Published Date 1983-08
Publication Title Acta Medica Okayama
Volume volume37
Issue issue4
Publisher Okayama University Medical School
Start Page 321
End Page 333
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6624532
Web of Science KeyUT A1983RE57600005
JaLCDOI 10.18926/AMO/32082
FullText URL fulltext.pdf
Author Tsuji, Takao| Araki, Kiyonori| Naito, Kunihiko| Inoue, Junichi| Nagashima, Hideo|
Abstract <p>Sera from 84 patients with chronic liver disease [CLD] (74 chronic active) and from 53 blood donors were tested immunochemically for anti-liver cell membrane antibody (LMAb). LMAb to rat liver tested by an indirect immunofluorescent technique was positive in 53.3% of CLD patients with positive HB surface antibody (HBsAb) and 40.0% of HBsAb positive blood donors. Pepsin digestion of the sera indicated that the binding between liver cell membrane and IgG was at the Fc site on the immunoglobulin. The sera with positive LMAb from HBsAb positive blood donors had elevated Clq-binding activity (Clq-BA). The LMAb to rat liver was a macro-molecular IgG (19-22S IgG) when assayed by Sephadex G-200 column chromatography and 5-40% sucrose density gradient ultracentrifugation. The results suggest that the LMAb in serum from a patient with chronic active liver disease may be an immune complex which consists of various antigens such as HB virus and its antibodies in serum.</p>
Keywords anti-liver cell membrane anitibody chronic active liver disease Fc receptor HB surface antibody immune complex
Amo Type Brief Note
Published Date 1979-02
Publication Title Acta Medica Okayama
Volume volume33
Issue issue1
Publisher Okayama University Medical School
Start Page 61
End Page 66
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 220848
JaLCDOI 10.18926/AMO/32077
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Hobara, Norio| Tobe, Kazuo| Endo, Hiroshi| Nagashima, Hideo|
Abstract <p>Sprague-Dawley rats given azathioprine in the diet for 3 to 4 weeks developed severe liver damage. Elevations of serum alkaline phosphatase and gamma-glutamyl transpeptidase activities were associated with increased hepatic glucose 6-phosphate dehydrogenase levels and decreased liver glucose 6-phosphatase activities, i.e., conditions which were commonly observed in various hepatotoxin-induced liver injuries. Light and electron microscopic observations revealed centrolobular necrosis with large scars and the proliferation of the mitochondria and rough endoplasmic reticulum. This model could be used to study the mechanisms of azathioprine-induced liver damage and its prevention.</p>
Keywords azathioprine liver injury mechanisms of hepatotoxicity phenobarbital microsomal enzymes
Amo Type Article
Published Date 1979-02
Publication Title Acta Medica Okayama
Volume volume33
Issue issue1
Publisher Okayama University Medical School
Start Page 5
End Page 14
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 220846
JaLCDOI 10.18926/AMO/32051
FullText URL fulltext.pdf
Author Tsuji, Takao| Naito, Kunihiko| Inoue, Junichi| Tsuchiya, Masao| Araki, Kiyonori| Shinohara, Toru| Onoue, Kimiaki| Nagashima, Hideo| Tokuyama, Katsuyuki| Okada, Takeshi|
Abstract <p>A study of 52 liver biopsies (47 hepatitis type B and 5 asymptomatic carriers) was performed to clarify the roles of HBe antigen (HBeAg), HB surface antigen (HBsAg) and HB core antigen (HBcAg). In this study, the Gudat classification was modified so as to classify the patterns of HB antigens into six reaction types including: type O (negative for both liver HBsAg and liver HBcAg), type III-A (characterized by a spotty HBsAg pattern) and type III-B (characterized from a sub-lobular to lobular HBsAg localization pattern). This classification enabled accurate prediction of the prognosis of hepatitis. Patients with positive serum HBeAg had either minimal hepatitis with mild clinical features or chronic aggressive hepatitis with severe clinical features. Ten patients negative for both HBeAg and HBeAb were all positive for liver HBcAg. In all 3 patients on corticosteroid administrations liver tissue was markedly positive for HBcAg and serum was usually positive for HBeAb.</p>
Keywords HBs antigen HBc antigen HBe antigen hepatitis B virus hepatitis type B chronic hepatitis type B chronic hepatitis
Amo Type Article
Published Date 1979-04
Publication Title Acta Medica Okayama
Volume volume33
Issue issue2
Publisher Okayama University Medical School
Start Page 121
End Page 131
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 157670
NAID 120002311621
JaLCDOI 10.18926/AMO/32047
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Higashi, Toshihiro| Nagashima, Hideo|
Abstract <p>A reproducible animal model of fulminant hepatic failure was developed by intraperitoneal administration of D-galactosamine hydrochloride to Sprague-Dawley rats. Biochemical and morphological hepatic injury and brain edema resembled human fulminant hepatic failure. This model would facilitate further studies of the pathogenesis of brain dysfunction and evaluation of treatment in fulminant hepatic failure.</p>
Keywords fulminant hepatic failure brain edema massive liver injury hepatic encephalopathy
Amo Type Article
Published Date 1979-12
Publication Title Acta Medica Okayama
Volume volume33
Issue issue6
Publisher Okayama University Medical School
Start Page 443
End Page 450
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 161469
NAID 120002311355
JaLCDOI 10.18926/AMO/32038
FullText URL fulltext.pdf
Author Watanabe, Akiharu| Higashi, Toshihiro| Endo, Hiroshi| Nagashima, Hideo|
Abstract <p>Three patients at various stages of remission from leukemia died following the development of massive liver necrosis within only 4-6 days. All had either hepatitis B surface antigen or antibody in their sera, and two of them experienced severe epigastric pain before the onset of liver injury. Hepatitis B surface antigen appeared in two of these patients after remission from leukemia. Serum gamma-globulin levels increased with decreasing doses of prednisolone and other antileukemic drugs, and hepatic cell necrosis occurred extensively. Localization of hepatitis B surface antigen in their livers revealed a strong positive reaction in the phagocytic cells. These observations strongly suggest that hepatitis B virus may be causally related to the fulminant hepatic failure at least in two of the reported leukemic patients.</p>
Keywords fulminant hepatic failure leukemia hepatitis B surface antigen massive liver necrosis immunosuppressive agents
Amo Type Article
Published Date 1979-08
Publication Title Acta Medica Okayama
Volume volume33
Issue issue4
Publisher Okayama University Medical School
Start Page 245
End Page 257
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 158950
JaLCDOI 10.18926/AMO/32037
FullText URL fulltext.pdf
Author Watanabe, Makoto| Taketa, Kazuhisa| Nagashima, Hideo| Yamamoto, Yoshio|
Abstract <p>In cardiovascular diseases with potential atherosclerosis, the serum concentration of HDL cholesterol as determined by a precipitation method with dextran sulfate and Mg++ was lower while that of total cholesterol was normal or elevated. Treatment with a daily dose of 1,200 mg of Nicomol, a derivative of nicotinic acid, for 1 to 3 months increased the mean HDL cholesterol level by 3 to 5 mg/dl and reduced the total cholesterol level by 14 to 15 mg/dl and total/HDL cholesterol ratio by 0.8 (3 months) to 0.9 (1 month, p less than 0.05). Similar decreases in HDL cholesterol concentration were also found in parenchymal and obstructive liver diseases with normal total cholesterol values except in fulminant hepatitis and intrahepatic cholestasis.</p>
Keywords HDL cholesterol nicomol atherosclerosis liver diseases
Amo Type Brief Note
Published Date 1979-08
Publication Title Acta Medica Okayama
Volume volume33
Issue issue4
Publisher Okayama University Medical School
Start Page 323
End Page 326
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 227232
JaLCDOI 10.18926/AMO/31943
FullText URL fulltext.pdf
Author Fujiwara, Ryoji| Tobe, Kazuo| Nagashima, Hideo|
Abstract <p>Cellular immunity against human bile proteins was investigated by the leukocyte migration inhibition test (LMIT) with 13 primary biliary cirrhosis (PBC) patients, 10 chronic aggressive hepatitis (CAH) patients and 21 healthy adults. Hepatic bile taken from patients operated on for lithiasis of the biliary tract was fractionated into five fractions with Sepharose 6B gel. A subtoxic dose of each fraction was determined in the healthy adults, and used as the antigen for LMIT. Out of the 5 fractions, only the third fraction led to an LMIT positive response in 8 out of 11 (73%) PBC patients and in 1 out of 10 (10%) CAH patients. The difference between PBC and CAH was significant (p less than 0.005). The remaining 3 PBC patients with LMIT negative responses were all under D-penicillamine treatment. Antibody to each fraction was prepared in rabbits. Using the antibodies after absorption with human serum, the localization of the antigens which were present in each fraction was investigated immunohistochemically using human liver sections. The antigen to the anti-first fraction antibody was detected specifically in the epithelial cells of the bile ducts and the ductules, and the antigen to the anti-third fraction antibody was detected specifically on the membrane of the bile canalicules. The third fraction was fractionated into three fractions by Sephadex G-200 gel. Only the first of the 3 fractions showed an LMIT positive response in 3 PBC patients, and its molecular weight was determined to be about 500,000. It is concluded that PBC patients develop cellular immunity against canalicular-antigen-containing fractions but not ductal-antigen-containing ones.</p>
Keywords primary biliary cirrhosis leukocyte migration inhibiton test bile protein canalicular antigen ductal antigen.
Amo Type Article
Published Date 1986-02
Publication Title Acta Medica Okayama
Volume volume40
Issue issue1
Publisher Okayama University Medical School
Start Page 17
End Page 25
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3083652
Web of Science KeyUT A1986A190200003