このエントリーをはてなブックマークに追加
ID 30558
JaLCDOI
FullText URL
Thumnail fulltext.pdf 1.57 MB
Author
Yamada, Gotaro
Takahashi, Takashi
Mizuno, Motowo
Sakamoto, Yuji
Kaga, Kazumi
Kobayashi, Toshinari
Nagashima, Hideo
Abstract

A recently modified method using peroxidase labeled antibodies for light and electron microscopic demonstration of hepatitis B virus (HBV) was applied to the evaluation of hepatitis B surface antigen (HBsAg) on the surface of liver cells in biopsy specimens from 24HBsAg chronic carriers. Membranous distribution of HBsAg was demonstrated in diffuse or scattered hepatocytes in all 4 asymptomatic carriers and in 3 of the 20 patients with HBsAg-positive chronic active hepatitis or liver cirrhosis. In these patients with membranous expression of HBsAg, hepatitis B e antigen, Dane particles and DNA polymerase were often detected in sera, and large amounts of hepatitis B core antigen appeared in the liver. These results suggest that membrane-bound HBsAg may be expressed by the HBV genome. The ultrastructural study of liver cells showing membranous expression disclosed dense deposits of reaction product indicative of HBsAg on the cell membrane and/or on assembled particles within the extracellular space. In some hepatocytes showing both diffuse cytoplasmic and membranous expression of HBsAg, HBsAg-positive membrane of cisternae open to the intercellular space was connected with the liver cell membrane. These findings supported the conjecture that HBV associated antigens are integrated into the liver cell membrane.

Keywords
type B hepatitis
membrane-bound HBsAg
immunoelectron microscopy
peroxidase-labeled antibody method.
Amo Type
Article
Published Date
1980-06
Publication Title
Acta Medica Okayama
Volume
volume34
Issue
issue3
Publisher
Okayama University Medical School
Start Page
175
End Page
187
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT