JaLCDOI 10.18926/AMO/32778
FullText URL fulltext.pdf
Author Ogata, Masana| Nagao, Yutaka| Jitsunari, Fumihiko| Kitamura, Naoji| Okazaki, Tokio|
Abstract <p>In order to ascertain whether black-crowned night herons (BCNH), white heron (Plumed Egrets (PE)) and domestic fowls are infected by JE virus and they serve as infection source ofJE, hemoagglutination inhibiting antibody and its 2·ME sensitive antibody in the sera of these birds were determined. Physico-chemical nature of fowl's antibody of JE produced by natural infection and their maternal antibody in the sera of chicks were examined. The results are briefly summarized as follows. 1) As to the herons captured in Tsudaka Town, two out of six adult night herons and three out of the four chicks showed positive HI reaction. On the other hand, HI reaction in the sera of two adult white herons and three chicks were negative. 2) As to the herons captured in Okayama City, twenty out of thirtytwo adult night herons and seven out of seventy white herons showed positive HI reaction in 1966 around the time when JE was prevalent in Okayama Prefecture. And six out of eleven night herons and one out of seven white herons showing positive HI reaction, responded positively to 2-ME sensitivity test. 3) The results indicate that white herons can be also infection source ofJE though less than in the case of night herons. 4) In the domestic fowls (white leghorn) kept at Takahashi District, eight out of twenty-seven fowls showed positive HI reaction. And six out of seven domestic fowls showing positive HI reaction responded positively to 2-ME sensitive reaction. 5) Transformation of JE antibody in the serum of hen from IgM to IgG was recognized. 6) Domestic chicken's sera having 1 : 640 of HI titer in the original serum and 1 : 320 of HI titer after 2-ME treatment were fractionated by gel filtration on Sephadex G-200 and the antibody activities present in the various fractions were determined. HI antibody activities occurred in both IgM and IgG classes of immunoglobulins. 7) Maternal HI antibodies reacting with JE virus were found in newly hatched domestic chickens from the eggs laid by hens with natural infection ofJE. And half life of HI antibodies in chicks was four days. 8) HI antibodies of JE in the serum of maternal immune-hens and chicken having maternal antibody were located in r-globulin fraction by starch block electrophoresis. 9) The results from 4) to 8) indicate the presence of natural infection ofJE in the domestic fowls. And domestic fowls can be infection source ofJE.</p>
Amo Type Article
Published Date 1970-04
Publication Title Acta Medicinae Okayama
Volume volume24
Issue issue2
Publisher Okayama University Medical School
Start Page 175
End Page 184
NCID AA00041342
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4318407
NAID 120002311429
JaLCDOI 10.18926/AMO/32768
FullText URL fulltext.pdf
Author Ogata, Masana| Nagao, Yutaka| Kikui, Ritsuko| Kitamura, Naoji|
Abstract <p>Transformation of Japanese encephalitis antibody from IgM to IgG in the sera of the experimental infected chicks with Japanese encephalitis virus and transmission of IgM or IgG from hen to chicks were examined by the gel filtration on Sephadex G-200 column. The following results were obtained. 1. Titer of hemoagglutination inhibiting antibody rose on seven days after inoculation of mouse brain homogenate infected with Japanese encephalitis, and that increased rapidly after the second inoculation of Japanese encephalitis. The maximum peak of antibody titer attained on 35 days after the first inoculation, on 7 days after the second inoculation and it maintained for a period of 2 months then decreased. Viremia was detected till 6 hours after the first inoculation. 2. IgM antibody by gel filtration appeared on 7 days after the first inoculation, kept on rising, reached the peak on 35 days after the first inoculation, then decreased, and disappeared on 120 days. IgG antibody appeared about 2 weeks after the IgM antibody appearance, and the titer of IgG antibody became higher than that of IgM antibody on 35 days after the first inoculation, then decreased gradually, and showed 1 : 16 of titer of peak on 150 days by gel filtration. 3. We could obtain the chicks by fertilization from experimentally infected hen, having IgM and IgG of hemoagglutination inhibiting antibody of Japanese encephalitis. And the localization of antibodies in the sera of its chicks was determined by Sephadex G-200 gel filtration. And IgG antibody was detected in chick serum, though IgM antibody was not detected by this method.</p>
Amo Type Article
Published Date 1970-12
Publication Title Acta Medicinae Okayama
Volume volume24
Issue issue6
Publisher Okayama University Medical School
Start Page 589
End Page 596
NCID AA00041342
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4334579
NAID 120002312139
JaLCDOI 10.18926/AMO/32766
FullText URL fulltext.pdf
Author Ogata, Masana| Nagao, Yutaka| Jitsunari, Fumihiko| Kikui, Ritsuko| Kitamura, Naoji|
Abstract <p>As to trial toward the elimination of Japanese encephalitis virus in natural surroundings, pigs received inoculation of inactivated Japanese encephalitis vaccine supplemented with complete Freund's adjuvant twice at one-week interval. Effect of adjuvant supplement on the magnitude of antibody and also prevention of viremia caused by natural infection by antibody induced with vaccine were investigated. The results of this study are summarized as follows. 1. In the group of pigs inoculated with vaccine containing adjuvant, titer of hemoagglutination inhibiting and neutralizing antibodies was higher than those inoculated with vaccine alone and their high titer persisted. 2. With respect to natural infection of pigs, on August 22 when the pigs were thought to have been infected, there was observed a rise in antibody titers. And on antibody formed in those pigs inoculated with vaccine with or without adjuvant proved to be all 2-ME resistant type, whereas the antibodies produced in control group were 2-ME sensitive antibody. 3. Viremia was detected in the blood of pigs naturally infected, but it was not demonstrated pigs inoculated with vaccine supplemented with adjuvant or without adjuvant. The virus of pig blood which was inoculated into suckling mouse brain and was separated after low suckling passage mouse was supposed to be JaGAr strain from optimum hydrogen ion concentration of its hemoagglutination reaction. 4. Effect of vaccination on antibody response of pigs having maternal antibody was not recognized.</p>
Amo Type Article
Published Date 1970-12
Publication Title Acta Medicinae Okayama
Volume volume24
Issue issue6
Publisher Okayama University Medical School
Start Page 579
End Page 587
NCID AA00041342
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4334578
NAID 120002312045
JaLCDOI 10.18926/AMO/32523
FullText URL fulltext.pdf
Author Ogata, Masana| Nagao, Yutaka| Jitsunari, Fumihiko| Kitamura, Naoji|
Abstract <p>As a step towards the elimination of Japanese encephalitis virus in natural surroundings, we inoculated pigs, rabbits and chicks with inactivated Japanese encephalitis vaccine supplemented with complete or incomplete Freund's adjuvant twice at one-week interval. Subsequently, we compared HI antibody titers of the groups inoculated with vaccine containing complete Freund's adjuvant (pigs, rabbits, chicks), of the group inoculated with vaccine containing incomplete adjuvant (rabbits), ar;d of the groups inoculated with vaccine containing no adjuvant (pigs, rabbits, chicks), and also observations on changes in the antibody titers due to natural infection. In a certain portion of these animals neutralizing antibody titers were also determined. The results of this study are briefly summarized as follows. 1. In the groups of pigs and rabbits inoculated with vaccine containing complete Freund's adjuvant, titers of HI antibody and neutralizing antibody were higher than those inoculated with vaccine containing no adjuvant and their high titers persisted. Further, in the group of chicks inoculated with inactivated Japanese encephalitis vaccine containing complete Freund's adjuvant, HI antibody titers were higher and persistent as compared with the antibody titers in the chicks inoculated with inactivated Japanese encephalitis vaccine alone. 2. In the rabbits inoculated with inactivated Japanese encephalitis vaccine contammg incomplete adjuvant, HI antibody titers were lower than in those receiving the vaccine with complete adjuvant, but it has been demonstrated clearly that vaccination of inactivated Japanese encephalitis vaccine supplemented with incomplete adjuvant brings about less sideeffects. Hence such a method of vaccination can be applied as the vaccination with least side-effects. 3. With respect to natural infection of swine, on August 27 when the pigs were thought to have been infected, there was observed a rise in antibody titers. And on being infected with Japanese encephalitis, the antibodies formed in those pigs inoculated with inactivated Japanese ence- phalitis vaccine with or without complete adjuvant proved to be all 2-ME resistant type, whereas the antibodies produced in the control groups not receiving such a vaccination were 2-ME sensitive antibody.</p>
Amo Type Article
Published Date 1969-12
Publication Title Acta Medicinae Okayama
Volume volume23
Issue issue6
Publisher Okayama University Medical School
Start Page 541
End Page 551
NCID AA00041342
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4316346
NAID 120002311752
JaLCDOI 10.18926/AMO/31180
FullText URL fulltext.pdf
Author Ogata, Masana| Nagao, Yutaka| Kikui, Ritsuko| Kitamura, Naoji| Ariyasu, Hajime| Kimoto, Hajime| Hagaya, Hisashi|
Abstract <p>In an attempt to eliminate Japanese encephalitis virus in natural surroundings, pigs having maternal antibody were given inoculation of live-attenuated Japanese encephalitis vaccine and injection of Freund's complete adjuvant simultaneously. Titer of hemoagglutination inhibiting antibodies of pigs inoculated with live attenuated vaccine and complete adjuvant, was higher than that inoculated with vaccine alone and its titer persisted.</p>
Amo Type Article
Published Date 1971-02
Publication Title Acta Medicinae Okayama
Volume volume25
Issue issue1
Publisher Okayama University Medical School
Start Page 71
End Page 75
NCID AA00041342
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4334469
NAID 120002312541