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ID 55190
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11
Title Alternative
Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis
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Author
Aizawa, Sayaka Graduate School of Natural Science and Technology, Okayama University
Higaki, Yuriko Graduate School of Natural Science and Technology, Okayama University
Ogoshi, Maho Graduate School of Natural Science and Technology, Okayama University Kakenhi
Takeuchi, Sakae Graduate School of Natural Science and Technology, Okayama University Kakenhi publons
Takahashi, Sumio Graduate School of Natural Science and Technology, Okayama University Kakenhi
Abstract
Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development.
Note
寄稿(Contribution)
Publication Title
Proceedings of Okayama Association for Laboratory Animal Science
Published Date
2017-04
Volume
volume33
Publisher
岡山実験動物研究会
Publisher Alternative
Okayama Association for Laboratory Animal Science
Start Page
31
End Page
34
Content Type
Journal Article
OAI-PMH Set
岡山大学
language
日本語
File Version
publisher
Refereed
False
Eprints Journal Name
poalas