JaLCDOI 10.18926/AMO/32904
FullText URL fulltext.pdf
Author Pazarbasi, Ayfer| Kasap, Mülkiye| Güzel, Ali Írfan| Kasap, Halil| Onbasioglu, Meliz| Özbakir, Burcu| Demirkazik, Ayse| Özgünen, Fatma Tuncay| Gürtunç, Evrim|
Abstract The genetic background predisposing pregnant women to pre-eclampsia/eclampsia (PE/E) is still unknown. The aim of the current study was to investigate whether there is an association between the TNF-alpha-308 and 850 polymorphisms and PE or eclampsia. In this study, 40 cases of eclampsia, 113 cases of PE and 80 normotensive control cases were genotyped for the TNF-alpha-G-308A and C-850 polymorphisms. At position 308, the replacement of Guanine with Adenosine was denoted as TNF2. We found a significant difference between the TNF2 allele frequencies of the eclamptic, pre-eclamptic and normotensive controls. TNF2 (AA) polymorphism frequency was significantly higher among the eclamptics and pre-eclamptics (control : 5%, PE : 13.3%, E : 12.9%). A significantly different genotype distribution of C-850T polymorphism was observed between the PE/E and control groups, with the frequency of the variant TT genotype being significantly reduced in the preeclamptics (PE : 17% ; E : 17.5%) when compared with the control group (24.3%). We have demonstrated an association between TNF-alpha polymorphisms and pre-eclampsia susceptibility. However, it is not known whether C-850T polymorphism has a functional effect on the TNF-alpha gene. In addition, it was not possible to determine whether this polymorphism promotes the progression from PE to eclampsia because of no statistically significant difference between eclampsia and the controls.
Keywords TNF-alpha polymorphisms eclampsia pre-eclampsia
Amo Type Original Article
Published Date 2007-06
Publication Title Acta Medica Okayama
Volume volume61
Issue issue3
Publisher Okayama University Medical School
Start Page 153
End Page 160
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 17593951
Web of Science KeyUT 000247574700004
JaLCDOI 10.18926/AMO/30735
FullText URL fulltext.pdf
Author Alptekin, Davut| Luleyap, Husnu Umit| Yilmaz, Levent| Demirhindi, Hakan| Gokel, Yuksel| Pazarbasi, Ayfer| Dokur, Mehmet| Kasap, Mulkiye| Kasap, Halil|
Abstract <p>This study included 45 patients with intentional insecticide intoxication and 21 with accidental intoxication who were treated at the First-Aid and Emergency Department of Balcali Hospital at the Faculty of Medicine in the Cukurova University, Adana, Turkey, while the control group consisted of 25 people selected from university personnel known to be healthy. Patients with a history of X-ray exposure in the last 6 months or of any virus disease as well as continuous drug users and smokers were excluded, leaving a total of 49 patients. Acetylcholine esterase (Pseudocholinesterase) enzyme (AchE), sister-chromatid exchanges (SCE), the mitotic index (MI), and the replication index (RI) were evaluated. Blood samples were cultured for SCE evaluation and sera separated for AchE levels. Insecticide exposure was generally intentional for suicide in adolescents and at older ages, but accidental for children. AchE levels were found to be significantly lower in organophosphorus (OP) and carbamated (CB) insecticide poisoning groups in comparison with the control group (p&#60;0.001), while the pyrethroid (PY) group was not statistically different for the AchE effect (p&#62;0.05). SCE was found to be significantly higher in OP and CB groups (p&#60;0.001), while the PY and control groups were statistically similar for SCE levels (p&#62;0.05). This study showed an increase in SCE in response to orally ingested insecticides. These findings indicate that insecticide exposure results in cell abnormalities, with resulting impediments to the division and replication of cells, as suggested by MI decreases and RI increases, while the speed of the division cycles of stimulated cells increases.</p>
Keywords insecticide intoxication acetylcholine esterase enzyme (AchE) sister-chromatid exchanges (SCE)
Amo Type Article
Published Date 2006-04
Publication Title Acta Medica Okayama
Volume volume60
Issue issue2
Publisher Okayama University Medical School
Start Page 121
End Page 126
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16680189
Web of Science KeyUT 000237001900008