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ID 18662
Eprint ID
18662
FullText URL
Thumnail 72_1581.pdf 13.7 MB
Title Alternative
STUDIES ON TISSUE CULTURES OF HUMAN AND MOUSE TUMORS Part 2. Carbohyrate Metabolism of Human Cervical Carcinoma, Bashford's Mouse Carcinoma, and Normal Mouse Liver Tissue in Tissue Culture
Author
Shiraishi, Akinori
Abstract
The roller-tubo tissue cultures of human cervical carcinoma, Bashford's mouse carcinoma, and normal mouse liver tissue were performed in order to inquire into carbohydrate metabolism in the culture media. Glucose consumption, lactic acid, pyruvic acid, and α-ketoglutaric acid productions in cutlure media by human and animal tumors and by normal mouse liver tissue were estimated, and the following results have been obtained. 1. Glucose consumption and lactic acid production by human cervical carcinoma and Bashford's mouse carcinoma were in higher level than by normal mouse liver tissue. This was considered as the fact showing the high glycolysis of tumor tissues in vitro. 2. The ratios of lactic acid production/glcose consumption, pyruvic acid production/glucose consumption, α-ketoglutaric acid production/glueose consumption, in each tissue, were compared with each other. The ratio of lactic acid production/glucose consumption in every tissue turned out to be quite similar in values. But the ratios of pyruvic acid production/glucose consumption and α-ketoglutaric acid production/glucose consumption in Bashford's mouse carcinoma, were very low, about one third and one tenth of normal mouse liver tissue, respectively. In human cervical carcinoma, the ratio of pyruvic acid production/glucose consumption showed almost the same value as normal mouse liver tissue, but the ratio of α-ketoglutaric acid production/glucose consumption in human cervical carcinoma was half the value of normal mouse liver tissue. Thus pyruvic acid and α-ketoglutaric acid production, the components of tricarboyylic acid cycle, were reduced in tumor tissuen in vitro inspite of the same glucose consumption and lactic acid production as in normal mouse liver tissue in vitro. Therefore, the carbohydrate metabolism, as for the tricaroxylic acid cycle in tumor tissues, turned out to be different from normal mouse liver tissue. 3. The changes of hydrogen ion concentration in the culture media were in step with the course of tissue cultures. And the pH in tissue culture media had a correlation with the lactic acid production by tissues in vitro. But the increase in the pH was closely correlated with the lactic acid, pyruvic acid, and α-ketoglutaric acid productions by human cervical carcinoma, Bashford's mouse carcinoma, and normal mouse liver tissue in culture media.
Published Date
1960-10-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume72
Issue
issue8-10
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1581
End Page
1593
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/72/8-10/72_8-10_1581/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma