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ID 19378
Eprint ID
19378
FullText URL
Thumnail 71_1909.pdf 753 KB
Title Alternative
Cytochemical Studies on the Mitochondria of Blood Cells Part 2. Cytochemical Study on the Cytochrome Oxidase System
Author
Sakai, Akira
Abstract
It is difficult to make an accurate cytochemical demonstration of the cytochrome oxidase system in the mitochondria of blood cells by the conventional Nadi reaction. By applying the Oda's method for the cytochemical, detection of cytochrome c-cytochrome oxidase system and cytochrome c oxdase the author succeeded in demonstrating cytochemically the cytochrome c oxidase or the cytochrome c-cytochrome oxidase system in the mitochondria of blood cells by using p-phenylenediamine as substrate and neotetrazolium salt as hydrogen acceptor with or without the addition of cytochrome c. This reaction appears in the cytoplasm of all blood cells with an exception of mature erythrocytes in the form of distinct violet-colored round or rod-like granules coinciding with mitochondria. The number, distribution and the grades of the enzyme activity of mitochondria can be seen. whose pictures are specific to each call species. Maturation and differentiation of cells generally proceed with the decrease both in number and enzyme activity.
Published Date
1959-04-05
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue4-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1909
End Page
1913
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/4-2/71_4-2_1909/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
OAI-PMH Set
岡山大学
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Sort Key
23
Eprints Journal Name
joma