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ID 46915
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Author
Hamada, Takahiro
Yoshimoto, Kaori
Murata, Takashi
Hasebe, Mitsuyasu
Watanabe, Yuichiro
Hashimoto, Takashi
Sakai, Tatsuya
Abstract
NimA-related kinase 6 (NEK6) has been implicated in microtubule regulation to suppress the ectopic outgrowth of epidermal cells; however, its molecular functions remain to be elucidated. Here, we analyze the function of NEK6 and other members of the NEK family with regard to epidermal cell expansion and cortical microtubule organization. The functional NEK6-green fluorescent protein fusion localizes to cortical microtubules, predominantly in particles that exhibit dynamic movement along microtubules. The kinase-dead mutant of NEK6 (ibo1-1) exhibits a disturbance of the cortical microtubule array at the site of ectopic protrusions in epidermal cells. Pharmacological studies with microtubule inhibitors and quantitative analysis of microtubule dynamics indicate excessive stabilization of cortical microtubules in ibo1/nek6 mutants. In addition, NEK6 directly binds to microtubules in vitro and phosphorylates beta-tubulin. NEK6 interacts and co-localizes with NEK4 and NEK5 in a transient expression assay. The ibo1-3 mutation markedly reduces the interaction between NEK6 and NEK4 and increases the interaction between NEK6 and NEK5. NEK4 and NEK5 are required for the ibo1/nek6 ectopic outgrowth phenotype in epidermal cells. These results demonstrate that NEK6 homodimerizes and forms heterodimers with NEK4 and NEK5 to regulate cortical microtubule organization possibly through the phosphorylation of beta-tubulins.
Keywords
NimA-related kinase
cell expansion
microtubule
epidermal cell
Arabidopsis thaliana
Published Date
2011-09
Publication Title
The Plant Journal
Volume
volume67
Issue
issue6
Start Page
993
End Page
1005
ISSN
0960-7412
Content Type
Journal Article
Official Url
http://dx.doi.org/10.1111/j.1365-313X.2011.04652.x
language
英語
File Version
author
Refereed
True
DOI
Web of Sience KeyUT