Author Takahashi, Fumio| Hatase, Osamu| Kuramitsu, Makoto| Itano, Toshifumi| Nisida, Isamu|
Published Date 1977-02
Publication Title Acta Medica Okayama
Volume volume31
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/32056
FullText URL fulltext.pdf
Author Hatase, Osamu| Fujii, Toshitake| Kuramitsu, Makoto| Itano, Toshifumi| Takahashi, Fumio| Murakami, Tetsuhide| Nishida, Isamu|
Abstract Factors that inhibit and stimulate cell proliferation were found to coexist in rat liver supernatant. The inhibitory and stimulatory factors were separated by ethanol fractionation. Both factors were sensitive to heat- and trypsin-treatment. The activity of the inhibitor was diminished by partial hepatectomy. The inhibitor from normal livers inhibited DNA and RNA synthesis in the L-cell system, but the same fraction from regenerating livers caused little or no inhibition of nucleic acid synthesis. The stimulatory factor from regenerating livers had a stronger effect on cell proliferation than that of normal livers. Furthermore, the inhibitor from normal livers depressed DNA synthesis in vivo in regenerating livers.
Keywords growth factors growth stimulants growth inhibitants rat liver cytoplasm cell proliferation
Amo Type Article
Published Date 1979-04
Publication Title Acta Medica Okayama
Volume volume33
Issue issue2
Publisher Okayama University Medical School
Start Page 73
End Page 80
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 157671
JaLCDOI 10.18926/AMO/32071
FullText URL fulltext.pdf
Author Takahashi, Fumio| Kuramitsu, Makoto| Tokuda, Masaaki| Matsui, Hideki| Itano, Toshifumi| Murakami, Tetsu-Hide| Hatase, Osamu| Nishida, Isamu|
Abstract <p>Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.</p>
Keywords chick growth factors cell proliferation growth regulation DNA and RNA synthesis protein synthesis
Amo Type Article
Published Date 1979-06
Publication Title Acta Medica Okayama
Volume volume33
Issue issue3
Publisher Okayama University Medical School
Start Page 167
End Page 176
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 158945