JaLCDOI 10.18926/AMO/32293
FullText URL fulltext.pdf
Author Jin, Zaishun| Teramoto, Norihiro| Yoshino, Tadashi| Takada, Kenzo| Oka, Takashi| Hayashi, Kazuhiko| Akagi, Tadaatsu|
Abstract <p>It has been reported that Epstein-Barr virus (EBV) resides in resting B cells in vivo. However, an ideal in vitro system for studying EBV latent infection in vivo has not yet been established. In this study, a mantle cell lymphoma line, SP53, was successfully infected with a recombinant EBV containing a neomycin-resistant gene. The EBV-carrying SP53 cells were obtained by selection using G418. They expressed EBER-1, EBNAs, and LMP1; this expression pattern of the EBV genes was similar to that in a lymphoblastoid cell line (LCL). However, proliferation assay showed that the EBV-carrying SP53 cells have a doubling time of 73 h, compared with 57 h of SP53 cells. Transplantation of 10(8) SP53 cells to nude mice formed tumors in 4 of 10 mice inoculated, but the EBV-carrying SP53 cells did not. Unexpectedly, EBV infection reduced the proliferation and tumorigenicity of SP53 cells. However, the EBV-carrying SP53 cells showed higher resistance to apoptosis induced by serum starvation than did the SP53 cells. The inhibition of proliferation and the resistance to apoptosis induced in SP53 cells by EBV infection indicate that this cell line might to some extent provide a model of in vivo EBV reservoir cells.</p>
Keywords Epstein-Barr virus mantle cell lymphoma latent infection in vivo reservoir SP53 line
Amo Type Article
Published Date 2000-10
Publication Title Acta Medica Okayama
Volume volume54
Issue issue5
Publisher Okayama University Medical School
Start Page 193
End Page 200
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11061568
Web of Sience KeyUT 000090098600002