JaLCDOI 10.18926/AMO/30788
FullText URL fulltext.pdf
Author Matsuo, Toshihiko| Takabatake, Mayumi| Matsuo, Nobuhiko|
Abstract <p>Retinal cells from chick embryos aged 7.5 days of gestation were cultured for two months in a non-adherent suspension culture dish to study the effects of growth factors and co-culture with retinal pigment epithelial cells on their differentiation. Dissociated retinal cells became cellular aggregates (multicellular spheroids) within a day, and rosettes were formed in the spheroids after 2 days. Ultrastructurally, neurons of the rosettes developed connecting cilia, ellipsoids (accumulation of mitochondria), and external limiting membrane, indicative of their differentiation into photoreceptor cells. Epidermal growth factor enhanced the expression of rhodopsin by rosette-forming neurons, while basic fibroblast growth factor induced the growth of Mueller cells at 4 weeks, and their transdifferentiation into lens-epithelial-like cells at 8 weeks. Co-culture of retinal cells with retinal pigment epithelial cells enhanced the formation of rosettes in spheroids. Multicellular spheroids formed in a dish for suspension culture would provide a convenient in vitro system to examine differentiation and transdifferentiation of the retina.</p>
Keywords multicellular spheroids differentiation transdifferentiation retina retinal pigment epithelium growth factors
Amo Type Article
Published Date 1997-10
Publication Title Acta Medica Okayama
Volume volume51
Issue issue5
Publisher Okayama University Medical School
Start Page 251
End Page 260
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9359922
Web of Sience KeyUT A1997YD65300003