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ID 10573
Eprint ID
10573
FullText URL
Title Alternative
逆転写酵素・DNAポリメラーゼを用いたRT-PCRによる増幅反応で確認されたペッパーマイルドモットルウイルス(PMMoV)感染ピーマンの果実種子におけるウイルスの効果的な不活性化方法
Author
Hikichi, Yasufumi
Takeuchi, Shigeharu
Okumura, Ako
Nasu, Yoshiko
Okuno, Tetsuro
Suzuki, Kazumi
Abstract
A method for efficient inactivation Papper mild mottle virus (PMMoV) in harvested seeds of green pepper was examined based on the infectivity on the leaves of Nicotina glutinosa L.(a local host) and a reverse transcription and polymerase chain reaction (RT-PCR)-based amplification of the viral RNA. The seed homogenates from PMMoV-infected plants produced a large number of necrotic local lesion in N.glutinosa, but soaking the seeds in 10% (w/v) tri-sodium phosphate (Na3PO4) for 20 min or dry sterilization (70C,3h) effectivity eliminated the abundance of PMMoV. However, no necrotic lesion on N.glutinosa or the RT-PCR-basaed amplification was observed with seeds that has been disinfected by Na3PO4 in combination with dry sterilization.
Keywords
Capsicum annuum L.
pepper mild mottle virus (PMMoV)
RT-PCR
seed disinfection
seed transmission
Published Date
2004-02
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific reports of the Faculty of Agriculture, Okayama University
Volume
volume93
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture,Okayama University
Start Page
29
End Page
32
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
英語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa