JaLCDOI 10.18926/AMO/32088
FullText URL fulltext.pdf
Author Jin, Gui-Shan| Kondo, Eisaku| Miyake, Takayoshi| Shibata, Masao| Takashima, Takako| Liu, Yi-Xuan| Hayashi, Kazuhiko| Akagi, Tadaatsu| Yoshino, Tadashi|
Abstract <p>FKHRL1 (FOXO3a), a member of the Forkhead family of genes, has been considered to be involved in the development of breast tumors; however, the in vivo expression and activation status of FKHRL1 in breast tumors still remains unclear. We immunohistochemically demonstrated the expression and intracellular localization of FKHRL1 in human breast tumors by the novel anti-FKHRL1 antibody which is available for formalin-fixed paraffin-embedded specimens. In a total of 51 cases of benign tumors, FKHRL1 was diffusely expressed in all cases, and its intracellular localization was revealed to be cytoplasmic (inactive form) in 94% of cases of intraductal papillomas (16/17) and 91% cases of fibroadenomas (31/34), with a similar pattern to normal glandular epithelium. In invasive ductal carcinomas, 83% of the cases (93/112) diffusely expressed FKHRL1; however, unlike benign tumors, 71% of the cases (66/93) showed the nuclear-targeted, active form of FKHRL1. Moreover, activated FKHRL1 was predominantly observed in scirrhous (29/36, 81% of the cases) and papillotubular (30/38, 79% of the cases) subtypes, compared to the solid-tubular subtype (7/19, 37% of the cases). Furthermore, the cases with nuclear-targeted FKHRL1 showed a tendency to have lymph nodal metastasis with statistical significance (P < 0.0001). Thus, the activation of FKHRL1 seems to be recognized as one of the specific features of invasive ductal carcinoma of the breast.</p>
Keywords FKHRL1 intracellular localization breast tumors
Amo Type Article
Published Date 2004-08
Publication Title Acta Medica Okayama
Volume volume58
Issue issue4
Publisher Okayama University Medical School
Start Page 197
End Page 205
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 15551757
Web of Sience KeyUT 000223559700004