JaLCDOI 10.18926/AMO/30558
FullText URL fulltext.pdf
Author Yamada, Gotaro| Takahashi, Takashi| Mizuno, Motowo| Sakamoto, Yuji| Kaga, Kazumi| Kobayashi, Toshinari| Nagashima, Hideo|
Abstract <p>A recently modified method using peroxidase labeled antibodies for light and electron microscopic demonstration of hepatitis B virus (HBV) was applied to the evaluation of hepatitis B surface antigen (HBsAg) on the surface of liver cells in biopsy specimens from 24HBsAg chronic carriers. Membranous distribution of HBsAg was demonstrated in diffuse or scattered hepatocytes in all 4 asymptomatic carriers and in 3 of the 20 patients with HBsAg-positive chronic active hepatitis or liver cirrhosis. In these patients with membranous expression of HBsAg, hepatitis B e antigen, Dane particles and DNA polymerase were often detected in sera, and large amounts of hepatitis B core antigen appeared in the liver. These results suggest that membrane-bound HBsAg may be expressed by the HBV genome. The ultrastructural study of liver cells showing membranous expression disclosed dense deposits of reaction product indicative of HBsAg on the cell membrane and/or on assembled particles within the extracellular space. In some hepatocytes showing both diffuse cytoplasmic and membranous expression of HBsAg, HBsAg-positive membrane of cisternae open to the intercellular space was connected with the liver cell membrane. These findings supported the conjecture that HBV associated antigens are integrated into the liver cell membrane.</p>
Keywords type B hepatitis membrane-bound HBsAg immunoelectron microscopy peroxidase-labeled antibody method.
Amo Type Article
Published Date 1980-06
Publication Title Acta Medica Okayama
Volume volume34
Issue issue3
Publisher Okayama University Medical School
Start Page 175
End Page 187
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 6447986
Web of Science KeyUT A1980KE59200005
JaLCDOI 10.18926/AMO/31428
FullText URL fulltext.pdf
Author Tsuji, Takao| Tokuyama, Katsuyuki| Okada, Takeshi| Takata, Shinji| Naito, Kunihiko| Kosaka, Kiyowo| Takahashi, Takashi| Mayumi, Makoto|
Abstract <p>Hepatitis B core antigen (HBc Ag) and hepatitis B surface antigen (HBs Ag) were detected in the liver tissue of a patient with chronic aggressive hepatitis by the immunofluorescent complement technique. The presence of anti-HBc was examined by the same method in 67 human sera previously tested for HBs Ag, anti-HBs and s-GPT levels. HBc Ag was localized mainly in the nucleus and sometimes in the cytoplasm of the hepatic cells. HBs Ag was found only in the cytoplasm. The focal area of HBc Ag positive hepatic cells seemed to correspond to the HBs Ag positive cells. Double staining demonstrated the simultaneous presence of HBs Ag and HBc Ag in individual cells. Anti-HBc positive serum was found in 46 (68.7%) cases. Forty-eight (71.6%) indicated a combination of HBs Ag and anti-HBc.</p>
Amo Type Original Article
Published Date 1976-02
Publication Title Acta Medica Okayama
Volume volume30
Issue issue1
Publisher Okayama University Medical School
Start Page 61
End Page 67
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 135480
NAID 120002312695