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ID 340
Eprint ID
340
FullText URL
Title Alternative
カロチノイドの生化学的研究 (第2報)緑葉キサントフイル類の単離とその水酸基のGLCによる定量
Author
Takagi, Shigeaki
Tokutomi, Takashi
Shiroishi, Masahide
Abstract
As the chromatographic behavior of the fatty acid esters of green leaf xanthophylls was different to that of free xanthophylls, the fractionation and the isolation of these xanthophylls were carried out with application of this properties by silicagel column chromatogray, followed by determination of hydoroxyl groups in these xanthophylls using the quantitative analysis of fatty acid to be bound in GLC method. By these chromatographic method six fractions of green leaf xanthophylls were able to be obtained as esters in which at least two were considered to isolate. When the hydroxyl gloups of these fractions were subsequently estimated by absolutely quantitative determination of fatty acid with GLC, only one xanthophyll had four hydroxyl groups and others two. These conclusions partially agreed with the results of hydroxyl numbers of the known xanthophylls, that is, VI-1 fraction which was considered to correspond to the lutein with one hydroxyl group had two hydroxyl groups, while VII-3 corresponded to auroxanthin with two hydroxyl groups had the same number of the groups. It was deduced that the difference between the estimated and the known hydroxyl numbers probably accounts for both the oxidation of the carotenoids during the chromatographic procedure and the subsequent production of epoxide groups. Therefore, the usefulness of these methods for fractionation of green leaf xanthophylls and for decision of hydroxyl numbers is now not able to be judged. But, up to date, the such above method has not been used and then is considered to have methodological originality.
Published Date
1973
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific Reports of the Faculty of Agriculture Okayama University
Volume
volume42
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture, Okayama University
Start Page
63
End Page
70
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
英語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa