JaLCDOI 10.18926/AMO/30482
FullText URL fulltext.pdf
Author Satoh, Yasumasa| Higashi, Toshihiro| Nouso, Kazuhiro| Shiota, Tetsuya| Kinugasa, Nobuyuki| Yoshida, Keigo| Uematsu, Shuji| Nakatsukasa, Harushige| Nishimura, Yukio| Tsuji, Takao|
Abstract <p>Cathepsin B, a thiol protease, is involved in cancer metastasis. To clarify the role of cathepsin B in tumor progression in human colorectal cancer, the relationship between its activity, immunohistochemical staining, and clinical tumor progression was investigated. Cathepsin B activity in adenocarcinomas was significantly elevated compared with that in the tumor-bearing tissue. Furthermore, the tumor/tumor-bearing tissue (T/Tb) ratio of the activity was significantly higher than that of colorectal adenoma. Immunohistochemical studies demonstrated intense staining in the cancerous tissue. With respect to the clinical stage of tumors, the activity tended to be higher in tumors that had invaded the serosa or subserosa than in those that invaded the proper muscle. The results suggest that cathepsin B participates in the progression of human colorectal cancer, and its increased expression is a sensitive marker of the differentiation between colorectal adenoma and adenocarcinoma.</p>
Keywords cathepsin B colorectal cancer colorectal adenoma
Amo Type Article
Published Date 1996-12
Publication Title Acta Medica Okayama
Volume volume50
Issue issue6
Publisher Okayama University Medical School
Start Page 305
End Page 311
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8985467
Web of Science KeyUT A1996WA04500004
JaLCDOI 10.18926/AMO/30480
FullText URL fulltext.pdf
Author Satoh, Yasumasa| Higashi, Toshihiro| Nouso, Kazuhiro| Shiota, Tetsuya| Kinugasa, Nobuyuki| Yoshida, Keigo| Uematsu, Shuji| Nakatsukasa, Harushige| Nishimura, Yukio| Tsuji, Takao|
Abstract <p>Cathepsin B, a thiol protease, has been reported to be involved in cancer progression and metastasis. The suppressive effects of two kinds of protease inhibitors, leupeptin and dietary camostate (FOY-305), on tumorigenesis and progression in 1, 2-dimethylhydrazine (DMH)-induced rat colon neoplasm were examined in relation to tissue cathepsin B activity. Male Donryu rats were treated with leupeptin or FOY-305 during or after the administration of DMH. There were no significant differences in average tumor numbers among all DMH-treated groups. However, the percentage of small tumors was significantly higher in the group in which leupeptin was supplied during DMH administration. This trend was not recognized in the FOY-305-treated groups. The ratio of cathepsin B activity in the tumors to that in the tumor-bearing tissue (T/Tb) was significantly increased with increasing tumor size (P = 0.009). The cathepsin B activity levels in the tumor-bearing mucosa in the groups which received leupeptin or FOY-305 following DMH treatment were both significantly lower than that in the group which received neither protease inhibitor (P = 0.046 and P = 0.0067, respectively). The results obtained indicate that leupeptin may have suppressed tumor growth by lowering the tissue cathepsin B activity.</p>
Keywords cathepsin B colorectal cancer colorectal adenoma
Amo Type Article
Published Date 1996-12
Publication Title Acta Medica Okayama
Volume volume50
Issue issue6
Publisher Okayama University Medical School
Start Page 305
End Page 311
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8985466
Web of Science KeyUT A1996WA04500003
Author Kanzaki, Hiromitsu| Nouso, Kazuhiro| Miyahara, Koji| Kajikawa, Naoko| Kobayashi, Sayo| Sakakihara, Ichiro| Iwadow, Shota| Uematsu, Shuji| Okamoto, Ryoichi| Shiraga, Kunihiro| Mizuno, Motowo| Araki, Yasuyuki|
Published Date 2009-09-01
Publication Title Cases Journal
Volume volume2
Content Type Journal Article