JaLCDOI 10.18926/AMO/31112
FullText URL fulltext.pdf
Author Inoue, Hiroshi| Mizuno, Motowo| Uesu, Tokurou| Ueki, Toru| Tsuji, Takao|
Abstract <p>To clarify the events related to complement-mediated immune responses in human colorectal cancers, we immunohistochemically examined the distribution of decay-accelerating factor (DAF), CD59/homologous restriction factor 20 (HRF20), membrane cofactor protein (MCP) and terminal complement complex (TCC) in human colorectal adenomas and cancers, and then compared the findings with their distribution in normal colonic mucosa. In the normal mucosa, TCC was not present on epithelial cells. Whereas DAF and CD59/HRF20 were present only occasionally on the apical surfaces of normal epithelial cells, MCP was diffusely distributed on the basolateral surfaces of most epithelial cells of the colon. These findings suggest that MCP has a primary role in the regulation of complement activation on these cells. In adenoma cells, the expression of both DAF and CD59/HRF20 was enhanced. In cancer cells, the expression of CD59/HRF20 and MCP was diminished, whereas DAF expression was markedly enhanced. Since DAF was frequently stained in the lumen of the cancer glands, it was suggested that DAF was released into the colonic lumen in patients with colorectal cancer.</p>
Keywords complement regulatory protein decayaccelerating factor membrane cofactor protein homologous restriction factor 20 colorectal cancer
Amo Type Article
Published Date 1994-10
Publication Title Acta Medica Okayama
Volume volume48
Issue issue5
Publisher Okayama University Medical School
Start Page 271
End Page 277
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7532345
Web of Sience KeyUT A1994PP23600007
JaLCDOI 10.18926/AMO/30412
FullText URL fulltext_.pdf
Author Ueki, Toru| Mizuno, Motowo| Uesu, Tokurou| Kiso, Takahiko| Tsuji, Takao|
Abstract <p>To clarify the immunological function of 'M' (microfold or membranous) cells in the large intestine, we examined the expression of intercellular adhesion molecule-1 (ICAM-1) and HLA-class II antigens immunohistochemically in M cells and follicle-associated epithelia (FAE) covering isolated lymphoid follicles of the human colon in comparison with their expression in Peyer's patches of the small intestine. In Peyer's patches of the small intestine, ICAM-1 was not expressed on the epithelial cells covering the lymphoid follicles, but their cell surfaces were stained positively for HLA-DR. In contrast, colonic M cells expressed ICAM-1 on their cell surfaces but were negative for HLA class II antigens. By immunoelectron microscopy, ICAM-1 was seen to be distributed on the surface of microfolds, on the membranes of apical vesicles and on part of the basolateral plasma membranes of M cells, but was not expressed on adjacent FAE. These findings imply that the M cells in the colon and in Peyer's patches have different immunological roles. In addition, identification of ICAM-1 expression on the colonic M cells should help elucidate the pathogenesis of some inflammatory colonic diseases which appear to start in the lymphoid follicles of the colonic mucosa.</p>
Keywords ICAM-I M cell follicle-associated epithelial cells HLA antigen immunoelectron microscopy
Amo Type Article
Published Date 1995-06
Publication Title Acta Medica Okayama
Volume volume49
Issue issue3
Publisher Okayama University Medical School
Start Page 145
End Page 151
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7676845
Web of Sience KeyUT A1995RH05400005