JaLCDOI 10.18926/AMO/30435
FullText URL fulltext.pdf
Author Takahashi, Isao| Hayashi, Naoki| Nakamura, Toru| Matuzaki, Toshiro| Murase, Toshio| Osada, Ken| Takeuchi, Makoto| Ueki, Yasufumi| Nakada, Hiroshi| Yorimitsu, Seiichi| Kimura, Ikuro|
Abstract <p>The effects of uridine(UR) on the cell-killing activity of cytosine arabinoside(ara-C) against human leukemic cells, MOLT-4, and on ara-C accumulation in cells were studied. The 50% lethal dose(LD50) of ara-C as determined by clonogenic assay was decreased to 5.0 x 10(-8) mol from 9.0 x 10(-7) mol after 3 days exposure to 10(-3) mol of UR. The accumulation of 3H-ara-C at 24 and 48 h was significantly increased in culture medium containing 10(-8) mol of 3H-ara-C and 10(-3) mol of UR (5,129 +/- 123.5 vs 2,554 +/- 115.5 cpm/10(5) cells at 24 h, p less than 0.01, and 5,772 +/- 123.2 vs 1,372 +/- 51.8 cpm/10(5) cells at 48 h, p less than 0.01). It is noteworthy that cell-killing activity of ara-C against human leukemic cells was enhanced by the combination with a nucleoside(UR), but not with antileukemic agents.</p>
Keywords cytosine arabinosids uridine antileukemic effect accumulation of cytosine arabinoside
Amo Type Article
Published Date 1990-12
Publication Title Acta Medica Okayama
Volume volume44
Issue issue6
Publisher Okayama University Medical School
Start Page 329
End Page 331
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2075831
Web of Science KeyUT A1990EP70700008