JaLCDOI 10.18926/AMO/31093
FullText URL fulltext.pdf
Author Masuda, Shusaku| Watanabe, Hironobu| Morioka, Masaaki| Fujita, Yukitoshi| Ageta, Tomiko| Kodama, Hiroyuki|
Abstract <p>Both prolidase and prolinase from the human prostate were separated into two peaks by TSK DEAE-5PW chromatography. These peaks of prolidase isozymes I and II differed from each other in their responses to preincubation with Mn2+, their substrate specificity, optimal pH, and heat stability. The molecular weights of prolidases I and II were estimated to be 110,000 and 165,000, respectively, by gel filtration. Substrate specificity of prolinase peaks I and II was almost the same, but they differed in optimal pH and heat stability. The molecular weights of prolinases I and II were about 85,000 and 63,000, respectively. These results indicate that two isozymes of prolidase and of prolinase, which differ in various characteristics, are present in the human prostate.</p>
Keywords human prostate prolidase prolinase
Amo Type Article
Published Date 1994-08
Publication Title Acta Medica Okayama
Volume volume48
Issue issue4
Publisher Okayama University Medical School
Start Page 173
End Page 179
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7817771
Web of Science KeyUT A1994PE51400001