JaLCDOI 10.18926/AMO/31748
FullText URL fulltext.pdf
Author Watanabe, Jun-ichi| Arima, Terukatsu| Nagashima, Hideo|
Abstract <p>The analysis of bile acids in human bile was tried by high-performance liquid chromatography (HPLC). Bile acids in human bile were first prefractionated into free, glycine- and taurine-conjugated bile acids using a Seppak C18 cartridge and a piperidinohydroxypropyl Sephadex LH-20 (PHP-LH-20) column. Each fraction was then processed through a HPLC system consisting of a Zorbax ODS column and a 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSD) column. By these procedures the major 15 bile acids were clearly separated, and each bile acid of 10-125 ng was accurately analyzed. More than 400 times of analysis could be repeated on one 3 alpha-HSD column without loss of sensitivity. Thus the pretreatment through Seppak C18 and PHP-LH-20 made the HPLC analysis of human bile acids accurate and applicable to clinically obtained materials.</p>
Keywords bile acid high-performance liquid chromatography 3?-hydroxysteroid dehydrogenase immobilized column piperidinohydroxypropy1 Sephadex LH-20
Amo Type Article
Published Date 1987-04
Publication Title Acta Medica Okayama
Volume volume41
Issue issue2
Publisher Okayama University Medical School
Start Page 47
End Page 54
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2438901
Web of Sience KeyUT A1987H040200001