JaLCDOI 10.18926/AMO/32307
FullText URL fulltext.pdf
Author Wrobel, Maria| Ubuka, Toshihiko| Yao, Wen-Bin| Abe, Tadashi|
Abstract <p>The effect of exogenous thyroxine (T4) administration on the activity of rhodanese, cystathionase, and 3-mercaptopyruvate sulfurtransferase (MPST) in the mitochondrial and cytosolic fractions of mouse liver was investigated. Three groups of mice were treated for 6 consecutive days with subcutaneous injections of T4 (50 micrograms, 100 micrograms, and 250 micrograms per 100 g of body wt, respectively). The other 3 groups were given 100 micrograms of T4 per 100 g of body wt for 1, 2, or 3 days. The dose of 100 micrograms T4 per 100 g of body wt given for 6 days exerted the strongest effect on the activity of all of the investigated enzymes. In comparison to the control, rhodanese activity diminished in the mitochondrial fraction by 40% (P &#60; 0.05), cystathionase activity diminished in the cytosolic fraction by 15% (P &#60; 0.05), and MPST activity in the mitochondrial fraction was reduced by 34% (P &#60; 0.05), whereas cytosolic MPST activity was unaltered. Simultaneously, in the liver homogenate, elevated levels of ATP and sulfate were observed after 6 days of T4 administration. Thus, the present results seem to suggest that in the mouse liver, after 6 days of administration of 100 micrograms T4 per 100 g of body wt, the desulfuration metabolism of L-cysteine is diminished, which is probably accompanied by an increase in oxidative L-cysteine metabolism. The dose of 100 micrograms per 100 g of body wt administered for a shorter period, and the use of a lower dosage (50 micrograms T4 per 100 g of body wt) for 6 days had a stimulatory effect upon MPST activity level, and an increased level of sulfane sulfur was observed.</p>
Keywords thyroxine rhodanese 3-mercaptopyruvate sulfurtransferase cystathionase sulfane sulful
Amo Type Article
Published Date 2000-02
Publication Title Acta Medica Okayama
Volume volume54
Issue issue1
Publisher Okayama University Medical School
Start Page 9
End Page 14
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10709617
Web of Sience KeyUT 000085526000002