JaLCDOI 10.18926/AMO/31311
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Author Tsuji, Hideyuki| Shimomura, Hiroyuki| Fujio, Kozo| Wato, Masaki| Kondo, Junichi| Hasui, Toshimi| Ishi, Yasushi| Fujioka, Shin-ichi| Tsuji, Takao|
Abstract <p>To evaluate viral interference between hepatitis B and C, we studied coinfected patients serologically and molecular biologically. Twenty-seven patients positive for hepatitis B surface antigen (HBsAg) and anti-hepatitis C virus (HCV) antibody, were classified into Groups BC-L and BC-H according to DNA-polymerase activity (less or greater than 100 cpm, respectively). Patients with hepatitis B or C alone were also enrolled as controls. HCV-RNA was detected more often in Group BC-L than in Group BC-H. Genotype 1b of HCV was determined in 75% of Group BC-H, 87.5% of Group BC-L, and 70.7% of hepatitis C-only patients. Activity of DNA-polymerase in coinfected patients was lower in patients positive for HCV-RNA as compared with those negative. HBsAg titers tended to be lower in coinfected patients than in patients with hepatitis B virus (HBV) alone. In conclusion, in coinfection, HBV may suppress the replication of HCV and HCV appears to reduce the expression of HBsAg and probably suppresses HBV replication.&#60;/P&#62;</p>
Keywords hepatitis B virus hepatitis C virus double infection hepatitis B surface antigen hepatitis C virusRNA
Amo Type Article
Published Date 1998-04
Publication Title Acta Medica Okayama
Volume volume52
Issue issue2
Publisher Okayama University Medical School
Start Page 113
End Page 118
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9588227
Web of Sience KeyUT 000073363000007
JaLCDOI 10.18926/AMO/31121
FullText URL fulltext.pdf
Author Hasui, Toshimi| Shimomura, Hiroyuki| Tsuji, Hideyuki| Wato, Masaki| Tsuji, Takao|
Abstract <p>Recently, factors predicting the response to interferon (IFN) therapy against hepatitis C virus (HCV) have received much attention. To evaluate the usefulness of the quantitation of intrahepatic HCV RNA as a predictive marker of the response to IFN therapy, we compared the amount of intrahepatic HCV RNA with serum levels in 16 patients. Eleven patients who had 10(10) copies/g or more of intrahepatic HCV RNA had increased level of serum alanine aminotransferase (ALT) after IFN therapy, while 4 of 5 patients who had less than 10(10) copies/g of intrahepatic HCV RNA achieved sustained normalization of serum ALT level and were designated as complete responders. Four complete responders possessed significantly less HCV RNA in the liver parenchyma than partial and nonresponders (P = 0.010, Mann-Whitney U-test), but the amount of HCV RNA in the serum was not significantly different between those groups. In conclusion, the results suggest that the quantitation of intrahepatic HCV RNA is a better indicator of the response to IFN therapy than serum HCV RNA.</p>
Keywords hepatitis C virus interferon liver tissue quantitation polymerase chain reaction
Amo Type Article
Published Date 1994-06
Publication Title Acta Medica Okayama
Volume volume48
Issue issue3
Publisher Okayama University Medical School
Start Page 151
End Page 157
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7524269
Web of Sience KeyUT A1994NV04300006
JaLCDOI 10.18926/AMO/30992
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Author Ishikawa, Shigenao| Inaba, Tomoki| Mizuno, Motowo| Okada, Hiroyuki| Kuwaki, Kenji| Kuzume, Toshiaki| Yokota, Hitomi| Fukuda, Yasuyo| Takeda, Kou| Nagano, Hiroshi| Wato, Masaki| Kawai, Kozo|
Abstract <p>Upper gastrointestinal bleeding is a major adverse event of non-steroidal anti-inflammatory drugs (NSAIDs), and co-administration of proton pump inhibitors and H2 receptor antagonists has been established as a means of preventing such an eff ect. However, the incidence of bleeding associated with NSAID-induced ulcers under conditions where such strong anti-acid agents are used for prevention has yet to be clarified. We aimed to determine the annual incidence of serious upper gastrointestinal ulcer bleeding among Japanese patients in whom NSAIDs were used in our hospital. Before commencing the study, we recommended to all the physicians in our hospital the best method for caring for NSAID users, focusing on the concomitant use of proton pump inhibitors or H2 receptor antagonists. We conducted a cohort study involving 17,270 patients for whom NSAIDs had been newly prescribed. Bleeding from gastric ulcers was observed in 8 of the 17,270 patients using NSAIDs (0.05%). The pooled incidence rate for bleeding was calculated as 2.65 (95% confidence interval, 2.56-2.74) and 1.29 (1.27-1.31) per 1,000 patient years for low-dose aspirin and non-aspirin NSAID users, respectively. None of the bleeding ulcer patients required blood transfusion or were in serious condition. In conclusion, gastric ulcer bleeding occurred in low-dose aspirin or non-aspirin NSAID users, but its incidence was low and outcomes were not serious when adequate preventive measures were taken.</p>
Keywords hemorrhage non-steroidal anti-inflammatory drugs peptic ulcer prevention
Amo Type Original Article
Published Date 2008-02
Publication Title Acta Medica Okayama
Volume volume62
Issue issue1
Publisher Okayama University Medical School
Start Page 29
End Page 36
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 18323869
Web of Sience KeyUT 000253549500005
JaLCDOI 10.18926/AMO/30506
FullText URL fulltext.pdf
Author Wato, Masaki| Shimomura, Hiroyuki| Fujio, Kozo| Tsuji, Hideyuki| Kondo, Junichi| Fujioka, Shin-ichi| Ishii, Yasushi| Hada, Hajime| Tsuji, Takao|
Abstract <p>We purified an apurinic/apyrimidinic (AP) endonuclease from mouse ascites sarcoma (SR-C3H/He) cells. The enzyme showed nicking activity on acid-depurinated DNA but not on untreated, intact DNA. It also showed priming activity for DNA polymerase on both acid-depurinated and bleomycin-damaged DNA. The priming activity on bleomycin-damaged DNA was two times higher than that on an acid-depurinated DNA. The enzymatic properties indicate that the enzyme is a class II AP endonuclease having DNA 3' repair diesterase activity. The purified enzyme has a molecular weight of 39,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimal pH for AP endonuclease activity was 8.0 in 50 mM Tris-HCl buffer. The AP endonuclease activity depended on divalent cation such as Mg2+ and Co2+ ions, and was inhibited by 2 mM EDTA with no addition of the divalent cation. An appropriate concentration of sodium or potassium salt stimulated the activity. Partial digestion of the AP endonuclease with Staphylococcus aureus V8 protease produced 4 major peptide fragments which may be used for protein sequencing.</p>
Keywords hepatitis C ultracentrifugation immune complex interferon
Amo Type Article
Published Date 1996-06
Publication Title Acta Medica Okayama
Volume volume50
Issue issue3
Publisher Okayama University Medical School
Start Page 139
End Page 144
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8805852
Web of Sience KeyUT A1996UU60400004
JaLCDOI 10.18926/AMO/30409
FullText URL fulltext.pdf
Author Tsuji, Hideyuki| Shimomura, Hiroyuki| Wato, Masaki| Kondo, Junichi| Tsuji, Takao|
Abstract <p>To study the virological and serological characteristics of asymptomatic hepatitis C virus (HCV) carriers, 165 blood donors positive for antibody against HCV proteins by the second generation assay, were analyzed for their clinical backgrounds, serological reactivity against antigens derived from HCV by recombinant immunoblot assay, and the amount and genotype of HCV by the polymerase chain reaction. Compared with blood donors having abnormal levels of alanine aminotransferase (ALT), sera from the donors with normal levels of ALT reacted less frequently against NS4 antigens (anti-5-1-1: 34.4% vs. 54.5%, P = 0.0609; anti-c100-3: 34.4% vs. 56.1%, P &#60; 0.05). Also the positivity for antibodies against these antigens were more frequent in sera from donors with genotype 1b HCV-RNA than other genotypes (anti-5-1-1: 61.0% vs. 23.5%, P &#60; 0.01; anti-c 100-3: 61.0% vs. 26.5%, P &#60; 0.01). The prevalence of each genotype in blood donors with normal ALT levels was different from that in patients with advanced liver disease (P &#60; 0.05), genotype 1b being less and genotype 2a being more frequent. The number of HCV-RNA copies/0.5 ml in donors with normal ALT was 10(7.9 +/- 1.0) (n = 27) and that in patients with chronic liver disease was 10(7.4 +/- 0.8) (n = 116), the difference being statistically significant (P &#60; 0.05). In conclusion, the results of this study suggest that asymptomatic blood donors carrying HCV have the serological and virological characteristics different from the patients with advanced liver disease.</p>
Keywords hepatitis C virus blood donor asymptomatic carrier
Amo Type Article
Published Date 1995-06
Publication Title Acta Medica Okayama
Volume volume49
Issue issue3
Publisher Okayama University Medical School
Start Page 137
End Page 144
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7545861
Web of Sience KeyUT A1995RH05400004