JaLCDOI 10.18926/AMO/31076
FullText URL fulltext.pdf
Author Washida, Setsuo|
Abstract <p>The biological specificity of the endotoxin receptor on platelet membranes was examined. The binding indices of platelets in experimental endotoxemia which was induced by intravenous administration of endotoxin (Lipopolysaccharide of E. coli, Difco) to rabbits were found to be 30% of the control at 60 min after the injection. The result suggests that the endotoxin receptor of platelets was already occupied. The binding indices of human platelets were measured after pretreatment with pharmacologically active substances which were assumed to effect platelet activity. The binding of LPS to platelets showed competitive inhibition at pharmacologically effective doses, but other substances merely inhibited platelet activity. One interpretation is that there is a common receptor on platelet cell membranes for lipopolysaccharide of E. coli and endotoxin. The sensitivity to endotoxin in vivo and binding indices of platelets were examined in rabbits and guinea pigs since their response to endotoxin is almost opposite with regard to sensitivity. The binding indices of platelets from rabbits and guinea pigs showed a positive correlation with the endotoxin sensitivity. Those findings indicate that platelets play a key role in vivo in the clinical course of endotoxemia.</p>
Keywords endotoxin receptor platelet
Amo Type Article
Published Date 1978-07
Publication Title Acta Medica Okayama
Volume volume32
Issue issue3
Publisher Okayama University Medical School
Start Page 217
End Page 223
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 151487
NAID 120002312797
JaLCDOI 10.18926/AMO/31058
FullText URL fulltext.pdf
Author Washida, Setsuo|
Abstract <p>Binding of bacterial endotoxin to platelets, erythrocytes, lymphocytes and granulocytes was examined by using diffusion dialysis. Platelets, erythrocytes, lymphocytes and granulocytes were fractionated from normal human blood and the binding of endotoxin (LPS: Lipopolysaccharide of E. coli) to each cell fraction was measured at 4 degrees C and the binding efficiency was expressed as a binding index (%d4degreesC +/- SD). The binding index for each cell fraction was as follows; 10.2 +/- 1.6 for platelets, 1.0 +/- 0.9 for erythrocytes, 4.3 +/- 1.6 for lymphocytes and 10.0 +/- 1.5 for granulocytes (n = 11) respectively. Since a platelet possesses a small cell surface area compared with other cells, it was clear that the endotoxin bound preferentially to platelets in vitro. The binding mechanism to the platelet cell surface was suggested to be direct binding of endotoxin to the receptor on platelet cell membrane rather than through an immunologically activated mechanism.</p>
Keywords endotoxin platelet receptor binding diffusion dialysis
Amo Type Article
Published Date 1978-06
Publication Title Acta Medica Okayama
Volume volume32
Issue issue2
Publisher Okayama University Medical School
Start Page 159
End Page 167
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 150201
NAID 120002312328