FullText URL Reprod_Fertil_Dev_29_10_1902.pdf
Author Bui, Tra M. T.| Nguyễn, Khánh X.| Karata, Asako| Ferré, Pilar| Trần, Minh T.| Wakai, Takuya| Funahashi, Hiroaki|
Abstract The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5-3mm diameter). When cumulus-oocyte complexes (COCs) from medium-sized follicles (MF; 3-6mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200ngmL-1 VEGF during the first 20h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200ngmL-1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200ngmL-1 during the first 20h of IVM.
Keywords pig
Note This is an Accepted Manuscript of an article published by CSIRO Publishing
Published Date 2016-12-12
Publication Title Reproduction, Fertility and Development
Volume volume29
Issue issue10
Publisher CSIRO Publishing
Start Page 1902
End Page 1909
ISSN 1031-3613
NCID AA10718189
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
File Version author
PubMed ID 27938625
DOI 10.1071/RD16321
Web of Sience KeyUT 000409376900003
Related Url https://doi.org/10.1071/RD16321