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ID 57385
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Author
Kaneta, Takashi Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID
Abstract
Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules. This review describes the CE-LIF methods developed by the author's group that include indirect and direct detection using diode lasers, post-column derivatization, and Hadamard transformation, as well as applications to the binding assays of specific DNA immunoassays of proteins and to the determination of anticancer drugs.
Keywords
DNA sequencing
capillary electrophoresis
laser-induced fluorescence
micellar electrokinetic chromatography
Published Date
2018-08-06
Publication Title
Chemical Record
Volume
volume19
Issue
issue2-3
Publisher
Wiley
Start Page
452
End Page
461
ISSN
15278999
NCID
AA11515019
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
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author
PubMed ID
DOI
Related Url
isVersionOf https://doi.org/10.1002/tcr.201800051