start-ver=1.4
cd-journal=joma
no-vol=16
cd-vols=
no-issue=2
article-no=
start-page=81
end-page=89
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200904
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Development of 5006 Full-Length CDNAs in Barley: A Tool for Accessing Cereal Genomics Resources
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=A collection of 5006 full-length (FL) cDNA sequences was developed in barley. Fifteen mRNA samples from various organs and treatments were pooled to develop a cDNA library using the CAP trapper method. More than 60% of the clones were confirmed to have complete coding sequences, based on comparison with rice amino acid and UniProt sequences. Blastn homologies (E < 1E-5) to rice genes and Arabidopsis genes were 89 and 47%, respectively. Of the 5028 possible amino acid sequences derived from the 5006 FLcDNAs, 4032 (80.2%) were classified into 1678 GreenPhyl multigenic families. There were 555 cDNAs showing low homology to both rice and Arabidopsis. Gene ontology annotation by InterProScan indicated that many of these cDNAs (71%) have no known molecular functions and may be unique to barley. The cDNAs showed high homology to Barley 1 GeneChip oligo probes (81%) and the wheat gene index (84%). The high homology between FLcDNAs (27%) and mapped barley expressed sequence tag enabled assigning linkage map positions to 151-233 FLcDNAs on each of the seven barley chromosomes. These comprehensive barley FLcDNAs provide strong platform to connect preexisting genomic and genetic resources and accelerate gene identification and genome analysis in barley and related species.
en-copyright=
kn-copyright=

en-aut-name=SatoKazuhiro
en-aut-sei=Sato
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=Shin-ITadasu
en-aut-sei=Shin-I
en-aut-mei=Tadasu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SekiMotoaki
en-aut-sei=Seki
en-aut-mei=Motoaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=ShinozakiKazuo
en-aut-sei=Shinozaki
en-aut-mei=Kazuo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=YoshidaHideya
en-aut-sei=Yoshida
en-aut-mei=Hideya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=TakedaKazuyoshi
en-aut-sei=Takeda
en-aut-mei=Kazuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=YamazakiYukiko
en-aut-sei=Yamazaki
en-aut-mei=Yukiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

en-aut-name=ConteMatthieu
en-aut-sei=Conte
en-aut-mei=Matthieu
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=

en-aut-name=KoharaYuji
en-aut-sei=Kohara
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=

affil-num=1
en-affil=
kn-affil=Okayama Univ, Bioresources Res Inst

affil-num=2
en-affil=
kn-affil=Natl Inst Genet

affil-num=3
en-affil=
kn-affil=RIKEN, Plant Sci Ctr

affil-num=4
en-affil=
kn-affil=RIKEN, Plant Sci Ctr

affil-num=5
en-affil=
kn-affil=Okayama Univ, Bioresources Res Inst

affil-num=6
en-affil=
kn-affil=Okayama Univ, Bioresources Res Inst

affil-num=7
en-affil=
kn-affil=Natl Inst Genet

affil-num=8
en-affil=
kn-affil=Int Rice Res Inst, Crop Res Informat Lab

affil-num=9
en-affil=
kn-affil=Natl Inst Genet
en-keyword=full-length cDNA
kn-keyword=full-length cDNA
en-keyword=Hordeum vulgare
kn-keyword=Hordeum vulgare
en-keyword=mRNA
kn-keyword=mRNA
en-keyword=gene ontology
kn-keyword=gene ontology
END