FullText URL viruses-09-00371.pdf
Author Ogawa, Hirohito| Kajihara, Masahiro| Nao, Naganori| Shigeno, Asako| Fujikura, Daisuke| Hang’ombe, Bernard M.| Mweene, Aaron S.| Mutemwa, Alisheke| Squarre, David| Yamada, Masao| Higashi, Hideaki| Sawa, Hirofumi| Takada, Ayato|
Abstract Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name "Bat mastadenovirus H". Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.
Keywords Eidolon helvum Zambia adenovirus bat
Published Date 2017-12-04
Publication Title Viruses
Volume volume9
Issue issue12
Publisher MDPI
Start Page 371
ISSN 1999-4915
Content Type Journal Article
language 英語
OAI-PMH Set 岡山大学
Copyright Holders https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
File Version publisher
PubMed ID 29207524
DOI 10.3390/v9120371
Related Url isVersionOf https://doi.org/10.3390/v9120371