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ID 57902
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4
Title Alternative
Mathematical analysis of copy number variation of 2 μ-based plasmids in yeast cells
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Author
Saeki, Nozomu Graduate School of Environmental and Life Science, Okayama University)
Moriya, Hisao Research Core for Interdisciplinary Sciences, Okayama University ORCID Kaken ID publons researchmap
Abstract
 Plasmids with the 2 μ plasmid origin are commonly-used in the genetic engineering of the budding yeast Saccharomyces cerevisiae. Intracellular copy numbers of 2 μ plasmids are different depending on the genes inserted into the plasmids. This difference is thought to occur from the difference in the growth efficiency (fitness) produced by the positive- and negative-selection biases of genes inserted in the plasmid. In this study, we made a mathematical model based on this assumption. Computational simulations of the model validated that copy numbers of the plasmids are rapidly settled depending on the fitness created by the gene on the plasmid. The copy number of a plasmid only contains a bias to keep the plasmid in a single copy became average 20copies per cell when the plasmid is randomly distributed, suggesting that no positive distribution mechanism is required for a plasmid to become multicopy.
Keywords
yeast
2 μ plasmid
mathematical model
Note
原著論文 (Original paper)
Publication Title
Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date
2020-02-01
Volume
volume109
Publisher
岡山大学農学部
Publisher Alternative
The Faculty of Agriculture, Okayama University
Start Page
7
End Page
11
ISSN
2186-7755
Content Type
Departmental Bulletin Paper
OAI-PMH Set
岡山大学
language
日本語
File Version
publisher
Eprints Journal Name
srfa