このエントリーをはてなブックマークに追加
ID 10600
Eprint ID
10600
FullText URL
Title Alternative
Molecular Mechanism of the Self-incompatibility in the Rosaceous Species
Author
Abstract
Self-incompatibility(GSI) in the rosaceous species is controlled by the S locus consisting of S-RNase gene and an unidentified 'pollen S' gene. A~200kbp of cosmid contig for the Sc haplotype of almond was constructed. Genomic Southern blot analyses showed that most cosmid end probes, except those near the Sc-RNase gene, cross-hybridized with DNA fragments from different S haplotypes, implying that the cosmid contig extends to the borders of the S locus. A~70kbp segment of the Sc haplotype, the S haplotype-specific region containing the S-RNase gene, was complentely sequenced. This regin was found to contain two pollen-expressed F-box genes that are likely candidates for pollen S genes. One of them, named SFB(S haplotype-specific F-Box protein), was specifically expressed in pollen, and showed high level of S haplotype-specific sequence polymorphism, comparable to that of the S-RNases. The other is unlikely to determine the S specificity of pollen, because it showed little allelic sequence polymorphism and was expressed also in pistil. Three other S haplotypes were cloned and the pollen-expressed genes were physically mapped. In all four cases, SFBs were physically linked to the S-RNase genes, and were located at the S haplotype-specific region, suggesting that the two genes are inherited as aunit. These features are consistent with the hypothrsis that SFB is the pollen S gene. This hypothesis predicts involvement of the ubiquitin/26S proteasome proteolytic pathway in the RNase-based SI system.
Keywords
self-incompatibility
S-RNase
SFB
F-Box protein
ubiquitin proteasome proteolytic pathway
Published Date
2005-02-01
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific reports of the Faculty of Agriculture, Okayama University
Volume
volume94
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture,Okayama University
Start Page
85
End Page
90
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa