Title Alternative Mathematical analysis of copy number variation of 2 μ-based plasmids in yeast cells
FullText URL srfa_109_007_011.pdf
Author Saeki, Nozomu| Moriya, Hisao|
Abstract  Plasmids with the 2 μ plasmid origin are commonly-used in the genetic engineering of the budding yeast Saccharomyces cerevisiae. Intracellular copy numbers of 2 μ plasmids are different depending on the genes inserted into the plasmids. This difference is thought to occur from the difference in the growth efficiency (fitness) produced by the positive- and negative-selection biases of genes inserted in the plasmid. In this study, we made a mathematical model based on this assumption. Computational simulations of the model validated that copy numbers of the plasmids are rapidly settled depending on the fitness created by the gene on the plasmid. The copy number of a plasmid only contains a bias to keep the plasmid in a single copy became average 20copies per cell when the plasmid is randomly distributed, suggesting that no positive distribution mechanism is required for a plasmid to become multicopy.
Keywords yeast 2 μ plasmid mathematical model
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 7
End Page 11
ISSN 2186-7755
language 日本語
File Version publisher
FullText URL srfa_109_013_020.pdf
Author Nguyen Quang Co| Nguyen Thi Thanh Hien| Saitoh, Kuniyuki|
Abstract The field study was conducted in Nam Dong district, Thua Thien Hue province, Vietnam during May 2019 to observe the effects of SRI method on the rice yield and eradicate the factor affecting the yield. The study was carried out to evaluate the agronomic characteristics of rice, fresh and dry matter production, soil characteristics with a focus group discussion regarding cultivation by SRI and non-SRI methods. The soil analyses using standard measurements showed quite similar soil fertility in both SRI and non-SRI methods, while rice plants showed good agronomic performances and fresh and dry matter production in SRI method. At harvesting time, the yield and yield potentials of rice were greater in SRI than that in non-SRI methods. Focus group discussion found some factors that limited SRI development in Nam Dong district.
Keywords Actual yield Focus group discussion Filled spikelets SRI Weeding
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 13
End Page 20
ISSN 2186-7755
language 英語
File Version publisher
FullText URL srfa_109_021_027.pdf
Author Annah Khatenje Indeche| Yoshida, Yuichi| Miyaji, Daisuke| Goto, Tanjuro| Yasuba, Ken-ichiro| Tanaka, Yoshiyuki|
Abstract The objectives of this study were to (i) determine the optimum number of whole leaves to retain on a tomato plant for effective blossom-end rot (BER) management and (ii) explore the relationship between shoot calcium (Ca) and fruit Ca in non-defoliated plants in two different sized fruit cultivars, a large-fruited cultivar ‘Momotaro fight’ and a medium-fruited cultivar ‘Cindy sweet’. Treatments involved maintaining 18, 15 and 12 leaves on a plant. All lateral shoots were removed regularly throughout the growing period except the shoot closest to the flowering truss in the 18-leaf treatment. At the length of 10cm, these shoots were sampled for real time Ca determination using a hand held Ca2+ meter. In the plants defoliated to 18 leaves, BER was higher in ‘Momotaro fight’ at 10% compared to 2% in ‘Cindy sweet’. Fruit growth rate was significantly increased by defoliation in ‘Momotaro fight’, however no significant difference was observed among treatments in ‘Cindy sweet’. Defoliating to 12 leaves increased daily Ca transport rate by 59% and 37% in ‘Momotaro fight’ and ‘Cindy sweet’, respectively. Defoliating to 12 leaves increased the water-soluble Ca concentration in the distal part of fruit by 34% and 14% in ‘Momotaro fight’ and ‘Cindy sweet’, respectively. In the plants defoliated to 18 leaves where only old yellowish leaves were removed, a significant steady decrease was observed in the concentration of water soluble Ca in the distal part of fruit with increase in truss order. There was a significant linear relationship between water-soluble Ca concentration in the distal part of the fruit and Ca concentration in the lateral shoot of plants defoliated to 18 leaves. We conclude that under moderate water stress by root zone restriction and also certain other BER inductive conditions, defoliation to 12–15 leaves on a tomato plant should be a promising approach for decreasing BER incidence in susceptible large fruit cultivars.
Keywords BER management defoliation water-soluble Ca lateral shoot Ca root zone restriction
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 21
End Page 27
ISSN 2186-7755
language 英語
File Version publisher
Title Alternative Development of Effective Biological Treatment Process for Acid Mine Drainage
FullText URL srfa_109_029_036.pdf
Author Kamimura, Kazuo| Kanao, Tadayoshi|
Abstract Acid mine drainage (AMD) is acidic and generally enriched with iron, aluminum, sulfate and heavy metals, such as lead and cadmium. AMD is a growing problem of emerging concern that cause detrimental effects to the environment and living organisms. Yanahara mine in Misaki Town, Okayama, Japan, had mainly produced pyrite for sulfuric acid manufacture. Although it was closed in 1991, AMD is being generated from the mine now. A passive treatment based on the biological oxidation of ferrous iron is a promising strategy for AMD remediation. AMD from Yanahara mine is treated in a plant using iron-oxidizing bacteria, Acidithiobacillus ferrooxidans and Ferrovum spp. The AMD generation continues for several centuries with dramatic consequences on the receiving environments. Therefore, the development of sustainable and cost effective treatment process is required. A development of the effective biological treatment process with an iron oxidation reactor operated at pH 3.5 is described in this report. Economic aspects are also discussed.
Keywords acid mine drainage bioremediation iron-oxidizing bacteria microbial community Acidithiobacillus ferrooxidans
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 29
End Page 36
ISSN 2186-7755
language 日本語
File Version publisher
Title Alternative Sexuality in plants, unveiled from genome evolution
FullText URL srfa_109_037_040.pdf
Author Akagi, Takashi|
Abstract Sexual polymorphism, a main strategy to maintain genetic diversity within a species, has long been a major focus in biology. Notwithstanding, in plants, evolution of sexual systems and mechanisms underlying these transitions have been little unveiled. We have elucidated the molecular mechanism of sex determination in persimmons (Diospyros spp.), where the Y-encoded smRNA gene OGI can repress the female-determining gene MeGI, and also in kiwifruits (Actinidia spp.), where the Y-encoded two sex determinants, Shy Girl and Friendly Boy, control gynoecium and androecium development, respectively. Although the molecular functions of these determinants are distinct, they have common evolutionary scenarios involving transitions of sexual systems. In persimmon, a recent genome triplication (hexaploidization) in cultivated persimmon (D. kaki) derived “flexible” sexuality via establishing epigenetic layers on the two sex determinants. On the other hand, an ancient Diospyros-specific paleo-genome duplication (paleo-tetraploidization) enabled neofunctionalization in the proto-MeGI, via positive selection, to establish a new function as a sex determinant. In kiwifruit, one of the two sex determinants, Shy Girl, was derived from neofunctionalization via Actinidia-specific duplication event. These findings exemplify how plant-specific numerous duplication events can drive flexible genetic material whose variation can be selected for development of new sexual systems.
Keywords Sex determination Sex chromosome Polyploidization Genome evolution Tree crops
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 37
End Page 40
ISSN 2186-7755
language 日本語
File Version publisher
Title Alternative Genetic mechanism for soil-surface roots originating from a New Plant Type rice (Oryza sativa L.)
FullText URL srfa_109_041_047.pdf
Author Tomita, Asami|
Abstract A new method of using seedling trays to evaluate root angle distribution in rice (Oryza sativa. L) was developed. By using this method, the root angle distributions of 97 accessions were characterized into two cluster groups ; A and B. The numbers of accessions in group A were limited, and these were categorized as shallow rooting types including soil-surface root. Group B included from shallow to deep rooting types, including both Indica and Japonica Group cultivars, lowland and upland cultivars, and landraces and improved types. An introgression line YTH16 harboring chromosome segments from a New Plant Type cultivar IR65600-87-2-2-3 with genetic background of an Indica Group rice IR 64, was included in Group A. To clarify the genetic mechanism for soil-surface rooting, quantitative trait loci (QTL) analysis was performed using hybrid populations derived from a cross between IR 64 and YTH16. A total of 8 QTLs were detected in the 3 introgressed segments on chromosomes (chr.) 2, 5 and 7. Seven chromosome segment lines (CSLs) combining these 3 QTL regions were selected from the progenies. The 2 CSLs harboring a single region (excluding the CSL with a region on chr. 5) showed high soil-surface root scores and low root vertical angles (RVA) in comparison with IR 64. Four CSLs harboring 2 or 3 regions showed high scores and low RVAs in comparison with YTH16 and the CSLs harboring a single QTL region. These results indicated that the soil-surface and shallow rooting of YTH16 was controlled by the 2 major QTLs’ regions on chrs. 2 and 7, and that chr. 5 particularly played a role for supporting the effect with them.
Keywords root angle distribution soil-surface root New Plant Type QTL rice (Oryza sativa L.)
Publication Title Scientific Reports of the Faculty of Agriculture, Okayama University
Published Date 2020-02-01
Volume volume109
Start Page 41
End Page 47
ISSN 2186-7755
language 日本語
File Version publisher
Title Alternative The Duality and Dynamics of Situated Learning Theory: Analyzing Wenger’s Dissertation ‘Transparency’
FullText URL bgeou_173_001_009.pdf
Author Hirata, Yoshitsugu|
Abstract 本論は,ウェンガーの学位論文である「透明性」を分析することによって状況学習理論の両義的および動的特質を解明することを目指す。「透明性」は,ウェンガーによるエスノグラフィックな調査に基づいており,アリンスという保険会社の請求処理係の実践に焦点を当てている。そこで用いられる調整シートという人工物=表象は,給付金計算の業務をより容易にする一方で,電話対応業務を難しくしていた。ウェンガーは,人工物=表象が可視性と不可視性を同時にもたらし,両者が両義的な関係にあることを指摘する。人工物=表象のもたらす可視性を利用しつつ,不可視性を克服するためには,実践共同体の周辺性において様々な参加形態を展開する成員による意味の交渉が重要となる。可視性と不可視性のジレンマゆえに,意味の交渉は駆動され,実践共同体を変化と安定の双方に開いていく。
Keywords 状況的学習論 透明性 人工物=表象 可視性/不可視性 意味の交渉
Publication Title Bulletin of Graduate School of Education, Okayama University
Published Date 2020-02-21
Volume volume173
Start Page 1
End Page 9
ISSN 1883-2423
language 日本語
File Version publisher
Title Alternative Development of an Attention Bias Modification (ABM) Software Application for Anxious Individuals
FullText URL bgeou_173_011_022.pdf
Author 上田 紋佳| 澤山 郁夫|
Abstract 近年,不安症患者にみられる注意バイアスの減少を直接ターゲットとする注意バイアス修正法(ABM)と呼ばれる新しい治療法に関する研究が盛んに行われている。その中でも特に,インターネットやスマートフォンなどのアプリを利用して自宅でABMを実施させる研究が進んでいる。本研究の目的はモバイルデバイスでABMが利用できるアプリを開発することであった。予備調査によって,高不安者にとってのネガティブ語および中性語を特定し,データベースを作成した。そのデータベースからABMの効果が見込める単語を抽出し,iOSアプリを開発した。利用者のデータをもとに,今後の課題について議論した。
Keywords 注意バイアス修正法 注意バイアス アプリ 不安 感情語
Publication Title Bulletin of Graduate School of Education, Okayama University
Published Date 2020-02-21
Volume volume173
Start Page 11
End Page 22
ISSN 1883-2423
language 日本語
File Version publisher
Title Alternative Cognition of Solid Figure Size Depending on Learning Stage
FullText URL bgeou_173_023_029.pdf
Author 入江 隆| 飯石 佳名子|
Abstract 身の回りには様々な立体が存在し,我々はそれらの大きさを認識している。立体の大きさを認識するためにはその形状を理解(推定)し,高さや幅という一次元情報,面積の二次元情報,体積の三次元情報を取得し,それらを情報処理している。本研究の関心は,立体をあらゆる角度から観察できる環境において,それら三情報をどのように利用して大きさ認識を行っているのかということにある。二次元情報と三次元情報は単純な視覚情報だけではなく,立体の正確な形状理解と数学的知識が必要となる。学校教育において図形の学習が行われ,その順序は最初に「一次元認知」,その後「二次元認知」,「三次元認知」へと次元が上がっている。すなわち学習段階によって立体の大きさの認識が変化する可能性がある。本研究では,幼児,小学校低学年,小学校中学年,中学生,大学生を被験者として,立体固有の一次元情報,二次元情報,三次元情報と大きさ認識との関係を明らかにした。
Keywords 立体 大きさ 認識 学習段階
Publication Title Bulletin of Graduate School of Education, Okayama University
Published Date 2020-02-21
Volume volume173
Start Page 23
End Page 29
ISSN 1883-2423
language 日本語
File Version publisher
FullText URL bgeou_173_031_044.pdf
Author 江原 久美子|
Abstract 国吉康雄は,1931 年から1932 年にかけて帰朝した際,自らについて報じた記事をスクラップしていた。現在,そのスクラップ記事原本は福武コレクション国吉康雄アーカイブに保管されている。本稿では,そのスクラップ記事をはじめとした当時の報道記事,また当時発行された各種印刷物や手紙,写真から,帰朝中の国吉康雄の動向を検討した。その結果,国吉康雄は当時の日本の画壇から歓迎され,展覧会が大きな反応を得ていたこと,一方,日本ではアメリカの美術についてほとんど知られておらず,国吉を驚かせたことなどが改めて浮かび上がってきた。
Keywords 国吉康雄 有島生馬 藤田嗣治 福武コレクション国吉康雄アーカイブ 二科会
Publication Title Bulletin of Graduate School of Education, Okayama University
Published Date 2020-02-21
Volume volume173
Start Page 31
End Page 44
ISSN 1883-2423
language 日本語
File Version publisher
Title Alternative Bulletin of Archaeological Research Center Okayama University 2017
FullText URL 紀要2017.pdf
Author Nozaki, Takahiro| Minami, Kentaro| Yamamoto, Etsuyo| Suzuki, Shigeyuki| Yamaguchi, Yuji| Iwasaki, Shiho| 古環境センター| 吉田生物研究所|
Publication Title 岡山大学埋蔵文化財調査研究センター紀要
Published Date 2019-03-29
Volume volume2017
language 日本語
Copyright Holders Archaeological Research Center, Okayama University
File Version publisher
Pages v, 96 p.
JaLCDOI 10.18926/58032
Title Alternative Practice of Academic Writing Course using LMS
FullText URL biess_4_001_010.pdf
Author KOBAYASHI, Yuji|
Abstract  本稿では、LMS を活用したアカデミック・ライティング科目の実践例を報告し、ライティング支援に関する今後の方向性等について検討した。本授業でのLMS 活用内容は主に、①資料(授業スライドおよび参考資料)の配布、②授業時の課題・コメント収集、③原稿(草稿、第一次原稿、最終原稿等)の提出およびフィードバック、の3 点であった。LMS の活用は、授業内外において学生と教員とのコミュニケーションを活発にし、ライティング課題の作成を円滑に進めるうえで極めて有効であったが、教員側がある程度、LMS 活用能力を備えていなければならず、また、コース作成の労力もそれなりにかかるため、今後、より多くのアカデミック・ライティング担当教員にLMS を活用してもらうためには、こうした課題を克服していく必要があると考えられる。
Keywords アクティブラーニング ライティング能力育成 学習支援 授業時間外学修 Moodle
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 1
End Page 10
ISSN 2432-9665
language 日本語
File Version publisher
NAID 120006812098
JaLCDOI 10.18926/58033
FullText URL biess_4_011_030.pdf
Author WICKSTRUM, Yuuka|
Abstract This paper discusses how Zainichi Koreans developed their identities both within and outside the realm of the ethnic community and ethnic organisations. This study uses qualitative multimethods research based on interviews with members and non-members of Zainichi Koirean organisations and field observations. The examination on Zainichi Koirean organisation members mainly concentrates on lived experiences of members from one of the ethnic organisations of Zainichi Koreans, Chongryun. This study finds that although some young Koreans separate themselves from the organisation and the homeland, they remain in the community. Many Chongryun Koreans share a collective ethnic consciousness, which was based on members’ love for the Chongryun schools. While there is an internal division within the community in terms of political views, many members still have a strong sense of group solidarity. On the other hand, the identities of non-member respondents are remarkably diverse. While some of them are assimilated into Japanese society, others retain strong ethnic identities. Their identities are less affected by any ethnic organisations and they rarely share collective memories with other Zainichi Koreans. This study shows that social networks and experiences of ethnic community individuals have a key role in determining their identities and the ways in which they live their lives.
Keywords Zainichi Koreans ethnic associations ethnic community collective identity
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 11
End Page 30
ISSN 2432-9665
language 英語
File Version publisher
NAID 120006812099
JaLCDOI 10.18926/58034
FullText URL biess_4_031_037.pdf
Author MAHMOOD, Sabina|
Abstract The number of International Baccalaureate (IB) students applying to Japanese Universities is increasing. Okayama University is a Super Global Japanese National University which started IB admissions in 2012, and presently has 50 IB students in 11 faculties and 1 special program, in addition to 4 IB graduates. The IB journey began in 2012, and over a period of 7 years, Okayama University has taken multiple approaches to become IB student-friendly, by revising the IB admission policies as per IB student requirements; training faculty on IB education; exploring the differences in IB education and Japanese High School education; listening to IB student voices and familiarizing faculty about IB student backgrounds and characteristics. By providing IB student support, educating faculty and doing extensive IB research, Okayama University has established itself as a pioneer of IB admissions among the IB Organization, prospective IB students and parents, IB college counselors and other National Universities in Japan.
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 31
End Page 37
ISSN 2432-9665
language 英語
File Version publisher
NAID 120006812100
JaLCDOI 10.18926/58035
Title Alternative Development of Academic Japanese Training Course for Students at the Discovery Program for Global Learners, Okayama University
FullText URL biess_4_038_049.pdf
Author USHIDA, Eiko|
Abstract 岡山大学グローバル・ディスカバリー・プログラムは英語だけで学位が取れる新しいプログラムであるが, 制度上他学部開講の授業も履修することが可能である。全学日本語の日本語コースで日本語「を」学ぶ学生とともに他学部で日本語「で」学ぶディスカバリー生も徐々に増えている。そこで他学部の授業や教養科目を履修するディスカバリー生を対象にアカデミック・ジャパニーズ科目を新規開講することになった。Backward Design,Differentiated Instruction,Learning Strategy Instruction の手法を利用し,授業をこなし単位を取得することを目標に,学生が限られた日本語能力で最大限に対応できる「自分に合う」学習ストラテジーを開拓できるコースの開発と履修状況を報告する。
Keywords グローバル・ディスカバリー・プログラム アカデミック・ジャパニーズ Backward Design Differentiated Instruction 学習ストラテジー
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 38
End Page 49
ISSN 2432-9665
language 日本語
File Version publisher
NAID 120006812101
JaLCDOI 10.18926/58036
Title Alternative Discovery Program for Global Learners at Okayama University
FullText URL biess_4_050_071.pdf
Author Chung, Haeng-ja|
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 50
End Page 71
ISSN 2432-9665
language 日本語
File Version publisher
NAID 120006812102
JaLCDOI 10.18926/58037
Title Alternative A Study on the Korean Sentences in "Korinsuchi" published in Early Meiji Era
FullText URL biess_4_072_087.pdf
Author JIN, Namtaek|
Abstract 本稿では、明治前期に刊行された明治14年刊『交隣須知』(初刊本)、明治16年刊『再刊交隣須知』(再刊本)、白石氏蔵版『交隣須知』(宝迫本)の3本における韓国語文を対象に、初刊本と再刊本、初刊本と宝迫本の韓国語文の違いを調べる。分析結果をみると、① 初刊本から再刊本においては、韓国語文の誤りを訂正したものの、大多数の韓国語文は同じであること、② 初刊本と宝迫本においては、宝迫本には初刊本と違って見出し語がなく、対訳の日本語文を上段に示し、また韓国語文の変化が多いことがわかる。この研究は交隣須知関する基礎研究であり、今後明治期の朝鮮語学習書に関する研究に役立つと考えられる。
Keywords 朝鮮語学習書 交隣須知刊行本 浦瀬裕 宝迫繁勝
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 72
End Page 87
ISSN 2432-9665
language 日本語
File Version publisher
NAID 120006812103
JaLCDOI 10.18926/58038
Title Alternative Selection and Evaluation of Extensive Reading Books by Japanese Language Learners
FullText URL biess_4_088_100.pdf
Author Banno, Eri|
Abstract 本稿では,著者の所属する大学で開講された多読クラスの概要を紹介するとともに,その授業の履修者107 名の読書記録から,彼らの選書傾向,本の理解度,面白さの評価を報告する。履修者は授業が進むにつれ,段階的読み物から日本語母語話者用の読み物を読む割合が増えていった。また,理解度については,段階別読み物の理解度が高く,日本語母語話者向けの本の中では絵本の理解度が高かった。さらに,面白さについては,漫画の評価が最も高かった。データからはまた,日本語力の低い学習者には母語話者向けの本は適切な多読教材とは言い難いこと,今後日本語学習者のための多読教材を質量両面での充実する必要性があることも示唆された。
Keywords 多読 日本語学習者 書籍 理解度 面白さ
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 88
End Page 100
ISSN 2432-9665
language 日本語
File Version publisher
NAID 120006812104
JaLCDOI 10.18926/58039
Title Alternative The Impact of Topic Selection on the Use of Viewpoint Expressions An Analysis of Native Japanese Speaker Narratives
FullText URL biess_4_101_111.pdf
Author SUESHIGE, Miwa|
Abstract 本研究では、日本語母語話者11 名に10 のトピックに関するパーソナル・ナラティヴを実施し、視点表現の出現傾向について分析を行った。分析の結果、日本語教科書で、迷惑や被害を表す表現として導入される受身表現に加え、移動表現についても他者から受けた不快な行為を描写する際に用いられることが分かった。また、使役表現は、典型的な強制の意味よりは、授受表現「てもらう」との組み合わせで感謝を示す際に用いられていた。以上の結果から、教科書で視点表現が扱われているトピックと日本語母語話者の使用が認められたものが必ずしも一致しないことが示された。
Keywords 視点表現 トピック ナラティヴ 日本語母語話者 日本語教科書
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 101
End Page 111
ISSN 2432-9665
language 日本語
File Version publisher
NAID 120006812105
JaLCDOI 10.18926/58040
FullText URL biess_4_112_123.pdf
Author YAMAMOTO, Yumiko|
Abstract One of the targets under the Sustainable Development Goals (SDGs) aims to reduce and redistribute unpaid care work, which is mostly done by women, through the provision of public services and the promotion of shared responsibility. In February 2019, experts from academia and international organizations gathered at Okayama University for an international symposium focused on the issues around unpaid and paid care work in relation to gender equality and sustainable society. This paper will first briefly summarize the issues in care work, with a focus on childcare and elderly care in a Japanese context, followed by a discussion of the key points from the symposium related to care-centered economic and social policies.
Keywords Care aging gender Japan SDGs
Publication Title Bulletin of Institute for Education and Student Services, Okayama University
Published Date 2019-12-30
Volume volume4
Start Page 112
End Page 123
ISSN 2432-9665
language 英語
File Version publisher
NAID 120006812106