JaLCDOI 10.18926/40265
Title Alternative Studies on Paper-Analysis in the Field of Balneology (I) Studies on Iron in Serum by Means of Parper-Electrophoresis and Paper-Chromatography
FullText URL 022_037_054.pdf
Author Ishibashi, Maruo|
Abstract 1. The author showed by means of paper-electrophoresis using o-nitrosoresorcinmonomethylether (hereafter N. R. M. E.), a colourdeveloping reagent, that iron in serum combines with β-fraction of serum protein. 2. The author proposed a new method for the determination of serum iron by means of paper-chromatography. Procedure is as follows. In a centriguged tube, 0.5 ml. of clear serum is taken, acidified with 0.15 ml. of 20% HNO(3), and allowed to stand for 20 minutes. After addition of 0.2 ml. of saturated solution of CH(3)COONa and 1 ml. of acetic acid-sodium acetate buffer solution (pH: 3.8), the mixture is kept for at least 10 min. and filtered through Toyo-filter-paper No.5 A. The clear filtrate is taken in a stoppered test tube (the inside diameter: 1.2 cm.; height: 15 cm.). To this filtrate are added 0.2 ml. of 20% Hydroxylamine hydrochloride and 0.25 ml. of saturated aqueous solution of N. R. M. E. After being allowed to stand for at least 20 min., the mixture is shaken with 5 ml. of carbon tebrachloride. Then the excess of N. R. M. E. is removed. The supernatant aqueous solution (0.2 ml.) is used for a determination sample. The sample is placed on the paper (Toyo,filter-paper No.51 or 50) in a thin line at a distance of 10 cm. from the edge, and the spots are developed with 90 % ethylalcohol. After development for 4-6 hours, a green-coloured linear spot appsars at a distance of 12-16 cm. from the origin-point. Spot intensity at 670 mμ is measured with Natsume's densitometer, and from this, concentration of Fe can be estimat.ed on the standard graph (Fig. 5). 3, Effects of radioactive thermal spring bathing and internal use of vitriol water upon serum iron levels were studied with the result.s as follows. The above-mentioned author's method of determining the iron level in a small amount of serum is very convenient in investigating iron metabolism in the field of balneotherapy. The author examined changes of iron levels in serum after radioactive thermal bathing as well as after internal use of acid vitriol water. a) The iron levels in serum were maasurecl by the author's method before and 5, 30 and 60 minutes after the radioactive thermal bath (Rn-content: 10-30 Mache, 42-3°C., for 10 min,). The iron levels in the serum of healthy subjects showed no significant change after the thermal bathing. b) Yanahara Mineral Water (an acid vitriol water, pH; 2.2) contains 0.045 gm. of Cl(-), 12.35 gm. of SO(4)(--), 2.5 gm. of Fe(++)+Fe(+++), 0.0938 gm. of Al(+++) and 0.0001 gm. of Cobalt in one liter. i) Thirty ml. of Yanahara, water diluted with plain water to 200 ml. (Fe(++) content: ca. 75 mg.) and administered to health fasting subjects orally. As a control matter, 0.65 gm. of Glukon-F powder (Fe(++) content.: ca. 75 mg.) was then given. Blood samples were taken from the cubital vein before and 1, 3 and 6 hours after the intake of the dtriol water. Iron contents of the serum samples were measured by the author's paper chromatographic method. The results were shown in the table and the figure. The iron contents of the serum after the intake of the mineral water were higher than in the case of the control. ii) Two ml. of Yanahara vitriol water was administered to rabbits with the aid of a stomach tube, and serum iron levels wera determined before and 1, 3, 4 and 6 hours after the administration. An amount of 0.05 gm. of Glukon-F powder was dissolved in 5 ml. of plain water, and this solution was given to rabbits as a control. After the internal use of vitriol water, iron levels in serum rose and remained unexpactedly high for about 1-4 hours, in a manner that did not. correspond to the amount of iron in the given mineral water. So that it was suspected that the iron originally contained in the living subjects had been mobilized by the intake of vitriol water. Iron levels in serum after administration of a solution of Glukon-F powder were lower than in the case of the mineral water.
Publication Title 岡山大学温泉研究所報告
Published Date 1958-07
Volume volume22
Start Page 37
End Page 54
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40263
language 日本語
File Version publisher
NAID 40017532359
JaLCDOI 10.18926/40264
Title Alternative Clinical and Experimental Studies of Therapeutic Effects of Radioactive Thermal Bath on Peripheric Disorders of Circulation Part 1 The Effect of Radioactive Thermal Bath upon Serum Cholesterol Levels
FullText URL 022_001_036.pdf
Author Matsumoto, Kinshi|
Abstract The author investigated the effect of radioactive hot spring bath on serum cholesterol levels. The chemical compositions of the spring waters used are described in Table I. As experimental subjects healthy men and healthy white rabbits were used. The following results were obtained. 1) Single bath. The total and ester cholesterol levels in the serum were measured by a modification of Bloor's method before and 1/2, 1, 2 and 3 hours after the radioactive thermal bath (41-43°C., for 5 minutes). The concentration of total and ester cholesterol had decreased following radioactive thermal baths (Hisui-no-Yu and Kenkyusho-Sen), and remained at a decreased level for l/2 to 2 hours, but tended to return to normalcy within three hours from the time of the bath. (Fig. 2, 3, 4, 5.) The plain-water bath and vapour bath used as a control (41-43°C., for 5 minutes and 44-47°C. for 10-20 minutes respectively), had no significant effect on the blood cholesterol levels (Fig. 7 and 8). 2) Series of baths. The author examined weekly for 4 weeks the changes in serum cholesterol in healthy subjects following "a series of baths" in radioactive hot springs. The total and ester cholesterol levels of the serum decreased during the first and second weeks and tended to return to the initial levels during the third and fourth weeks (Fig. 11). The total cholesterol levels of the serum in rabbits following "a series of baths" in KenkyushoSen (Rn-content; 10-40 Mache) increased during the second week, but the cholesterol levels of the serum, after bathing in Hisui-no-Yu (Rn-content: 300-400 Mache), tended to decrease during the first and second weeks (Fig. 9 and 10). From this experiment, it is concluded that the content of radon in hot spring water affects the concentrations of the serum cholesterol levels. 3) Following an intramuscular injection of A. C. T. H. or Cortisone, the concentrations of total cholesterol and ester cholesterol had decreased and remained at the low level for 4 hours (Fig. 12. 13, 14 and 15). 4) It is said that the reticulo-endotherial system plays an important part in cholesterol metabolism. The author injectt 5 ml. of 1% Indian ink into the aureal vein of the rabbits for the purpose of blocking the reticulo-endotherial system. These procedures were carried out once a day for 7 successive days. The total cholesterol levels of the serum in the bathed group (bathing in Kenkyusho-sen once a day for 4 weeks, 43°C., 5 minntes) were lower than those of the control (Fig. 16). From these findings, the author thinks that the fall in the serum cholesterol levels following Misasa Hot spring bathing is due to the synthetic action of many factors such as the acceleration of oxydation in the living body, and the hyperfunction of liver cells, of hypophysealadrenocortical, of reticulo-endotherial systems and of various endocrine glands, caused by radioactive thermal bathing. Alternation of vegetative nervous system and various vitamine metabolism also take part in this phenomenon.
Publication Title 岡山大学温泉研究所報告
Published Date 1958-07
Volume volume22
Start Page 1
End Page 36
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40263
language 日本語
File Version publisher
NAID 40017532360
JaLCDOI 10.18926/40256
Title Alternative A Case of Gastric Polip
FullText URL 024_096_103.pdf
Author Matsumoto, Kinshi| Wakutani, Tohaku| Izumi, Tomokuni|
Abstract We examine patients seeking for spa treatment about erythrocyte sedimentation rate, Takata's reaction, blood picture, gastric juice, urinalysis and so on on their admittion and then repeat these tests once weekly to investigate the reaction of bathing in radioactive hot spring. A 65-year-old patient showed achylia gastrica every time in the test, but he complained no gastric symptom. On x-ray examination we found gastric polyp and verified this on operation.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-01
Volume volume24
Start Page 96
End Page 103
ISSN 0369-7142
language 日本語
File Version publisher
NAID 40017532365
JaLCDOI 10.18926/40255
Title Alternative A Case of Agammaglobulinemia
FullText URL 024_087_095.pdf
Author Matsumoto, Kinshi| Wakutani, Tohaku|
Abstract A case of agammaglobulinemia, probably of the secondary type, is reported in adult female (45-year-old) with tumor of the mediastinum. The authors described the bibliographical consideration and arose the attention of agammaglobulinemia. Reports on agammaglobulinemia may increase in practise if electrophoresis of the serum protein-fraction is used as a routine examination method.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-01
Volume volume24
Start Page 87
End Page 95
ISSN 0369-7142
language 日本語
File Version publisher
NAID 40017532364
JaLCDOI 10.18926/40253
Title Alternative Studies on Paper-analysis in the Field of Balneology. (3) The Changes of the Serum Protein Fractions and Lipoprotein Fractions Following the Bathing in Radioactive Hot Spring
FullText URL 024_054_079.pdf
Author Ishibashi, Maruo|
Abstract I. Some fundamental conditions and procedures on filter-paper electrophoresis were investigated. The electrophoretic apparatus of Natsume Seisakusho and Toyo No. 51 filter-paper were used. Electrophoresis was carried out under the current of 0.25 mA/cm. in 300 Volt during 5 hours, using veronal buffer pH 8.6 (μ=0.05). In measuring of serum protein fractions, the adequate volume of the materials loaded is in the range of 0.005~0.01 ml. and in the case of lipoprotein, 0.02 ml. of serum is used. Paper-strip was stained with bromophenol blue or sudan black B, then making it transparent with heat-solved paraffin and photometered by Nataume's densitometer. The reproducibility of the above-mentioned method with a confidence of 99% was as follows: Serum protein fractions Albumin 53.4±1.8% α(1)-Globulin 3.3±0.3 α(2)-Globulin 6.0±0.5 β-Globulin 12.2±0.7 γ-Globulin 25.1±1.1 Serum lipoprotein fractions α 20.6±2.6% β+γ 79.4±2.6 II. Changes in serum protein fractions and lipoprotein fractions following the radioactive hot spring bathing were as follows: 1). In rabbits, the albumin-fraction of serum protein increased after a series of baths in radioactive hot spring, and the β- and γ-globulin-fractions decreased. 2). By cholesterol-feeding, the albumin-fraction of serum protein of rabbits decreased and the β- and γ-globulin-fractions increased significantly but when a series of baths was carried out in cholesterol-fed rabbits the decrease in the albumin-fraction was slighter than the former. 3). α-globulin-fraction of serum lipoprotein increased after single bathing in radioactive hot spring and (β+γ) -globulin-fractions showed an inverse change, but the changes of lipoprotein-fractions following a series of baths were not remarkable. 4). By cholesterol-feeding, the ratio of lipoprotein-fractions (β+γ/α) increased, but the ratio tended to decrease following a series of baths in radioactive hot spring.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-01
Volume volume24
Start Page 54
End Page 79
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40250
language 日本語
File Version publisher
NAID 40017532366
JaLCDOI 10.18926/40252
Title Alternative Studies on Paper-analysis in the Field of Balneology. (II) 1. A New Method for the Determination of Cobalt in Blood. 2. Effects of Radioactive Thermal Bathing and Internal Use of Vitriol Water upon Cobalt Levels in Blood.
FullText URL 024_029_053.pdf
Author Ishibashi, Maruo|
Abstract 1. A new method for the determination of cobalt in blood. The author proposed a new method for the determination of cobalt in blood by means of paper-chromatography. The procedure is follows. In a Kjeldahl colben, 5 m!. of blood is taken, and turned into wet ash with 3 ml. of HNO(3), 0.5 ml. of H(2)SO(4) and 1.5 ml. of HClO(4) and the ash aqueus solution is then neutralized with ammonium hydroxid, the neutralization being indicated by the development of color of 0.1% of p-nitrophenol (one drop) added to the solution. After addition of 2 ml. of 40% ammonium citrate, 0.5 ml. of 20% Na(2)S(2)O(3), 3~5 drops of H(2)O(2) and 1 ml. of o-nitrosoresorcine monomethyl ether (hereafter N.R.M.E.), the mixture is left for at least 15 minutes and the aqueus solution is taken in a stoppered test tube (inside diameter: 1.3 cm., hight: 16.5 cm.). The mixture is shaken with 2 ml. of carbon tetrachloride. Carbon tetrachloride is shaken with 5 ml. of 20% Na(2)CO(3) solution. Then the excess of N.R.M.E. is removed and carbon tetrachloride is concentrated on water bath to 0.3~0.5 ml. and all CCl(4) is used for a determination sample. The sample is placed on the paper (Toyo-filter paper No. 50 or 51A) in a thin line at a distance of 3 cm. from the edge, and the spot is developed with a mixture of 20 ml. of CCl(4) and 1 ml. of 90% ethylalcohoI. After development for 30~40 min., an orange-colored linear spot appears at a distance of 9~10 em. from the origin point. Spot intensity is measured at 460 mμ with Natsume's densitometer and quantitative estimation is made comparing with the standard graph. 2. Effects of radioactive thermal spring bathing and internal use of vitriol water upon cobalt levels in blood. As the author's method above-mentioned is able to determine the cobalt level in a small amount of blood, it is very convenient in investigating cobalt metabolism in the field of balneology. The author examined the changes of cobalt levels in blood after radioactive thermal bathing as well as after internal use of acid vitriol water. a. By the author's method cobalt in normal human blood was 0.4~3.6γ/100ml. in man (average: 1.6±0.4γ/100ml.) and 0.2~2.2γ/100ml. in woman (average: 0.7±0.3γ/100ml.). b. The cobalt levels in blood were measured by the author's method before and 5, 30, 60 minutes after the radioactive thermal single bathing, and before and 1, 2, 3, 4 weeks after a series of radioactive thermal baths (Rn-content: 10~30 Mache, 42~3°C., for 10 minutes). The cobalt levels in the blood of healthy subjects showed no significant change after the thermal single bathing, but those of patients with rheumatoid arthritis showed a slight increase on 3rd or 7th day and a fall on 2nd week of a series of radioactive thermal baths and then tended to return to the initial levels during the 3rd and 4th weeks of spa treatment. c. Thirty ml. of Yanahara mineral water (an acid vitriol water, pH: 2.2) was diluted with plain water to 200 ml. (Co(++)content: ca.3γ) and administered to healthy fasting subjects orally. Blood samples were taken from the cubital vein before and 1, 3, 6 hours after the intake of the vitriol water. The cobalt levels in the blood showed an increase of 14% of the initial levels at 1 and 3 hours and a decrease of 22% at 6 hours after the drinking of Yanahara mineral water.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-01
Volume volume24
Start Page 29
End Page 53
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40250
language 日本語
File Version publisher
NAID 40017532366
JaLCDOI 10.18926/40251
Title Alternative Clinical and Experimental Studies of Therapeutic Effects of Radioactive Thermal Bath on Peripheric Disorders of Circulation Part 4 Therapeutic Effects of Radioactive Thermal Bath on Blood pressure
FullText URL 024_001_028.pdf
Author Matsumoto, Kinshi|
Abstract 1) The effects of a single bath in the radioactive hot spring on the blood pressure and the pulse rate. The author observed the changes of the blood pressure and the pulse rate of subjects bathing for 20 minutes, 39°± 1°C, in temperature, in the radioactive hot spring in Misasa. It may be summerized as follows (Table 2, Fig. 1). a. In the hypertensive group (maximal blood pressure······181 mm. Hg. and over), the maximal blood pressure had decreased immediately after bathing, and remained at this level even 2 hours after bathing. The minimal blood pressure reacted in the same manner as the maximal blood pressure. b. The moderate hypertensive group (maximal blood pressure······180-161 mm. Hg.). In a few instances, the maximal blood pressure had increased immediately after bathing, but decreased within 30-90 minutes after bathing. Changes in the minimal blood pressure were not so remarkable. c. In the normal group (maximal blood pressure······160~101 mm. Hg.), the maximal blood pressure had lowered within 30-90 minutes after the radioactive hot spring bathing. The minimal blood pressure showed no significant changes. b. The pulse rate had increased immediately after bathing in all groups, but within 30 minntes had returned to the initial rate and there were no further changes for 2 hours (Table 2, Fig. 2). 2) The effects of a radioactive vapour bath on the blood pressure and the pulse rate. After a bathing in vapour bath (10~15 minutes), the maximal and the minimal blood pressure showed mild decrease for 2 hours. The changes of the pulse rate and the pulse amplitude were not so remarkable (Table 3, Fig. 3). 3) The effects of a series of baths in the radioactive spring on the blood pressure. The author observed the changes in the blood pressure of the subjects bathed 2 or 3 times daily in the radioactive hot spring in Misasa, at a temperature of 42~43°C., 5~10 minutes. The blood pressure of subjects was measured once a day (at 7 a.m.) for the first week, and subsequently once weekly for two additional weeks. Subjects with an initial maximal blood pressure over 160 mm. Hg. showed a fall on the second day of the spa treatment followed by a temporary rise on the 3rd or 4th day, after which the pressure fell to the 2nd day level again, and then remained fairly constant to the end of the observation period. Subjects with an initial blood pressure under 159 mm. Hg. also showed a fall in the course of the spa treatment, but the degree of the fall was slighter than that of the former (Table 4, Fig. 4). This tendency was also observed on the out-patients (Table 5, Fig. 5). The changes of the blood pressure and pulse rate of subjects after a bath for 20 minutes, in water 39±1°C. in temperature, on the first day of the spa therapy were compared with those occuring on the 6th or 7th day, and it was observed that the patterns of the changes in blood pressure following bathing on the two days were significantly different (i.e. on the latter, so called "the initial blood pressure increase following the bathing" was not observed) (Table 6,7,8, Fig.6,7,8,9,). 4) The remote investigation on the spa visitors with hypertension and/or arteriosclerosis. The author investigated the course of patients with hypertension and/or arteriosclerosis who received the spa treatment at Misasa after returned to home. The incidence of subjects who answered as being good was 86%, and it was observed the fall of the maximal and the minimal blood pressure, especially the fall was remakable in subjects who stayed at spa about 4 weeks (Table 10, 11, 12, 13, 14, 15, Fig. 10). 5) The incidence of hypertension among the residents in the spa resort and those residing in other places. The author measured the blood presssre of the out-patients of his institute over 41 years of age from June, 1956 to May, 1957. The incidence of patients with the maximal blood pressure over 160 mm. Hg. was 16.4% in spa resort, and 25.7% in places where there are no hot springs. Using x(2)-test, the difference between the two was recognised as being significant (Table 16, 18). 6) Comparison of the mortality rate of patients with hypertension between residents in the spa resort and those residing in other places. The author examined the mortality rate of persons with hypertension in the area under the jurisdiction of the Kurayoshi Sanitary Institute, Tottori prefecture, from 1954 to 1956. The rate of mortality in the spa resort was 17.5% as compared to 21.4% in other places (Table17).
Publication Title 岡山大学温泉研究所報告
Published Date 1959-01
Volume volume24
Start Page 1
End Page 28
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40250
language 日本語
File Version publisher
NAID 40017532368
JaLCDOI 10.18926/40246
Title Alternative Studies on the Chemical Composition and Levels of Ground Water at Misasa Hot Springs
FullText URL 025_013_024.pdf
Author Sakanoue, Masanobu|
Abstract The water levels of the thermal springs and the ground water levels were measured and the contour of the water table was obtained. The contour was disturbed at two regions in which many springs issue. The thermal and ground waters were sampled all over the springs district, and the water temperatures were measured when they were sampled. The chloride, bicarbonate, sulfate, calcium and magnesium content was also determined. In addition, the analytical methods for the determination of arsenic, phosphorus and acid-soluble iron were improved. Also the concentration of these elements was determined. Based on the results of the observations and chemical analysis, the relations between the water temperatures and content of chemical constituent and the chloride content were checked respectively. Positive correlations were found in the relations between water temperature~Cl(-), HCO(3)(-)~Cl(-), SO(4)(2-)~Cl(-), Ca(2+)~Cl(-), and As~Cl(-). Furthermore, the regional differences were detectable in the ratios of water temperature to chloride content and of bicarbonate to chloride content, nevertheless the correlation as a whole were valid. The diversity and regional differences of the chemical composition of the waters were explained as due to the differences in the mixing proportion of the magmatic thermal water and the varying kinds of ground water at the deep site in the ground and at the site near the ground surfaces.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-05
Volume volume25
Start Page 13
End Page 24
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40244
language 日本語
File Version publisher
JaLCDOI 10.18926/40242
Title Alternative Clinical Studies on Anemia in Rheumatoid Arthritis (III)
FullText URL 026_050_063.pdf
Author Yamamoto, Yasuhisa|
Abstract III. The Effects of Balneotherapy and Antianemics on Anemia in Rheumatoid Arthritis 1. Balneotherapy: The author investigated the changes of the peripheral blood picture, erythrocyte sedimentation rate, numbers of finger-ring (Wheatsheaf's ring-test) and grasping power (by means of hand-dynamometer or sphygmometer) before and after baneotherapy. The regimen of spa treatment in our hospital includes bathing in radioactive hot spring (42-3°C. in temperature)two or three times a day, local mud bath or mud pack (once or twice a day, massage and drinking of spring water. These measures performed for one or two months. When the spa treatment has finished the erythrocyte count and Hb-content of rheumatoid patients increased in 61% of 33 cases examined comparing with before treatment, leucocyte count tended to shift to normal and E.S.R. decreased in 58% of the cases. The swelling of the finger joints diminished. 2. Oral iron therapy: In previous report, the author verified that there exist simple iron deficiency anemia in some cases of rheumatoid arthritis. Oral iron therapy responded well in such cases, but the majority of cases were resistant to oral iron. 3. Transfusion of blood in rheumatoid anemia: The author transfused 100 ml. of blood once a day for 5-10 days in 11 rheumatoid anemic patients. By the treatment of transfusion, the findings in peripheral blood improved and E.S.R. decreased.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-10
Volume volume26
Start Page 50
End Page 63
ISSN 0369-7142
language 日本語
File Version publisher
JaLCDOI 10.18926/40241
Title Alternative Clinical Studies on Anemia in Rheumatoid Arthritis (II)
FullText URL 026_031_049.pdf
Author Yamamoto, Yasuhisa|
Abstract II. Iron Metabolism 1. The serum iron: The serum iron was estimated by the method of Umemoto and Yamamoto (by means of o-nitroso resorcinmonomethylether) The average serum iron level of 33 cases with rheumatoid arthritis was 47γ/100 ml., and in 27 of 33 cases (82%) the serum iron levels were shown to be below the lower limit of the normal range (5% rejection limit: 54-141γ/100 ml. ), but the concentrations of serum iron of neuralgias (degenerative Spondylosis, sciatica and painful shoulder) were mostly within the normal range. 2. Iron absorption: To investigate the possible role of poor absorption in preventing a response to oral iron therapy, the changes in serum iron were followed after a test of iron by mouth in 16 caseS with rheumatoid arthritis. The test dose was 1 gm. of reduced iron given with 30 mI. of lemonade-pepsin solution to preserve the iron in the ferrous state and obviate the effects of possible achlorhydria in rheumatoid patients. The results may be grouped under three headings: a) Large rise in serum iron: good adsorption. Three caseS (19%) showed a very large rise in serum iron after the test dose, which is similar to simple iron-deficiency anemia. b) Slight rise in serum iron: presumed poor absorption. Six cases (38%) showed a very small or negligible rise after the test dose, so that at its peak the serum iron was below the normal range. c) In the remaining 7 cases, a maximal rise in serum iron after the test dose was within the normal range. 3. The iron-binding capacity of the serum: The iron-binding capacity of the serum was estimated by means of intravenous injection of Gluferricon (Fe content: 10 mg.). The mean total iron-binding capacity of the serum in the present 13 cases with rheumatoid arthritis was 273γ/100ml., virtually the same as the normal figure (287γ /100 ml.). The degree of saturation of the iron-binding protein with iron was found to lie between 18 and 45% (average: 34%) in control sUbjects and between 7 and 24% (average: 16%) in rheumatoid patients. 4. The serum copper: The serum copper was estimated by means of diethyldithiocarbamate in rheumatoid arthritis with the following results. Healthy subjects: men (15 cases): mean 89.9±14.8γ /l00 ml., women (15 cases): mean 99.9± 12.6γ/100 ml. There is no significant difference between the two. In 10 of 17 cases (57%) with rheumatoid arthritis, the serum copper levels were shown to be above the upper limit of the normal range (57-138γ/100 ml. ・・・5% rejection limit), neuralgias were mostly (92%) within the normal range. The results of investigations upon alternations in iron metabolism of rheumatoid anemia are reported. 1. The serum iron concentration was usually reduced. 2. The intestinal absorption of iron after a single dose of 1 gm. of reduced iron was variable. Strong presumptive evidence of impaired absorption waS recognised in many cases, but some cases gave the excellent absorption. From these results, it seems that simple iron deficiency exist in some anemic caseS of rheumatoid arthritis. 3. The total iron-binding capacity of the serum was slightly reduced. 4. The degree of Saturation of iron-binding protein with iron was below nornal. 5. The serum copper concentration waS usually elevated.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-10
Volume volume26
Start Page 31
End Page 49
ISSN 0369-7142
language 日本語
File Version publisher
JaLCDOI 10.18926/40240
Title Alternative Clinical Study on the Treatment of Rheumatoid Arthritis Part III. Experiences of Capsulosynovectomia Genu Anterior Totalis for Rheumatoid Arthritis
FullText URL 026_020_030.pdf
Author Izumi, Tomokuni|
Abstract Capsulosynovectomia genu anterior totalis was performed on five cases had marked hydrops and capsular thickening of joints, in which intraarticular injection of hydrocortisone or prednisolone, balneotherapy and hydrotherapy had been repeated in vain for long period with long standing pain and gradual decrease in motor function. The progress was observed for six to seventeen months after the operation, obtaining following results. Rheumatic symptoms were significantly improved after operation and restortion of function was also valuable. No exacerbations in the other joints and general condition were noted with rather improvement in the sign. Further, these operated joints became to react better, differ from preoperative, to balneotherapy and "Fango" with noticeable effects on the restoration of articular function. In histological findings of the resected synovia four cases showed Rs. and one Fr. by T. Kodama's classification. It is, therefore, recommended to appreciate this kind of operation on the cases shown no effects in the treatment of adrenocortical hormone or hot spring bath, which led to the decrease in gait ability, on the standpoint of medical rehabilitation.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-10
Volume volume26
Start Page 20
End Page 30
ISSN 0369-7142
language 日本語
File Version publisher
JaLCDOI 10.18926/40239
Title Alternative Clinical Study on the Treatment of Rheumatoid Arthritis Part II. Experiences of Intraarticular Injection of Prednisolone for Rheumatoid Arthritis
FullText URL 026_008_019.pdf
Author Izumi, Tomokuni|
Abstract Meticorterone (Prednisolone acetate) were injected into joints of 33 cases of rheumatoid arthritis, 172 times in total, with better results. Although intraarticular injection of prednisolone is, at present, to be one of the most effective treatment for rheumatoid arthritis, one must pay attention at the appearance of general effect in frequent and abundant injection. Comparative studies were performed on the effects of intraarticular injectons of prednisolone T. B. A, (on twelve cases), hydrocortisone acetate and meticorterone. Prednisolone T. B. A. was proved markedly longer effect in local with minimum general effect, It was, therefore, thought that prednisolone T. B. A. was safest and most effective in intraarticular therapy on the rheumatoid arthritis.
Publication Title 岡山大学温泉研究所報告
Published Date 1959-10
Volume volume26
Start Page 8
End Page 19
ISSN 0369-7142
language 日本語
File Version publisher
JaLCDOI 10.18926/40350
Title Alternative TWO CACES OF CHRONIC FOUDOL POISONING
FullText URL 013_044_050.pdf
Author Onda, Sakue| Kishida, Senzo|
Abstract Two cases of chronic Folidol intoxication were described. Both patients complained of oppressive feeling in upper abdomen, breast and head, since exposure to Folidol-spray. Case 1. A farmer aged 21. Two months after Folidol-spraying, investigation revealed a slight fever, a slight hyperchromic anaemia with relative lymphocytosis, achylia gafotrica, a decreased concentration in bile with a few gall-sands and an occasional tenderness on gall-bladder. Bromsulfalein test slightly positive (8%). A hypoglycaemia, hypotension, high sensitivity to pilocarpine, vanished patellar and Achilles tendon reflexes and a positive Sawada test in urine were also observed. None of the treatments given showed a favourable result. Case 2. A farmer, aged 27. Three months after Folidol-spraying, investigation revealed a slight fever, a hypochromic anaemia and a decrease in serum-cholinesterase activity (40%).
Publication Title 岡山大学温泉研究所報告
Published Date 1953-12
Volume volume13
Start Page 44
End Page 50
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40340
language 日本語
File Version publisher
NAID 40017532456
JaLCDOI 10.18926/40348
Title Alternative MEDICAL STUDIES ON CHONDROITIN SULFATE (III)
FullText URL 013_036_041.pdf
Author Oshima, Yoshio| Sotozono, Masazumi|
Abstract 2-5cc. of 1% Rodium chondroitin-sulfate solution subcutaneouRly or 2cc. of 5% solution intraveneously was administered to 37 cares of painful disorders such as headache, backache or arthralgia. A marked improvement was obtained in 12 cares and a moderate result was seen in 16 cases. No definite change was proved concerning blood pressure in the patients and detoxicating effect in animal experiment.
Publication Title 岡山大学温泉研究所報告
Published Date 1953-12
Volume volume13
Start Page 36
End Page 41
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40340
language 日本語
File Version publisher
NAID 40017532453
JaLCDOI 10.18926/40346
Title Alternative STUDIES ON SERUM MUCOPROTEIN (2) RELATION BETWEEN SERUM MUCOPROTEIN LEVEL AND DIFFERENTIAL AGGLUTINATION TEST FOR RHEUMATOID ARTHRITIS. AN EXPERIMENTAL AND CLINICAL STUDY
FullText URL 013_024_030.pdf
Author Ueda, Yoshio|
Abstract (1) Mucoprotein level in serum and agglutination reaction by Rose for rheumatoid arthritis were measured in 40 cases of rheumatoid arthritis, cancer, pulmonary tuberculosis or liver disease (mostly acute hepatitis). A raised titer of mucoprotein was often observed in cancer, and a marked rise in agglutination titer was often proved in rheumatoid arthritis. But no significant correlation was proved between the serum mucoprotein level and agglutination test in patients. (2) [n animal experiment an inereased agglutination titer was caused by sensitization with egg albumin, Arthus' s phenomenon, anaphylactic, shock, thermal spring bath, X-ray irradiation, blocking of reticuloendothelial system, liver injuries, injection of A. C. T. H., adrenaline, atropin or pilocarpin. A simultaneoas rise in serum mucoprotein level was observed after sensitization, thermal bath, X-ray irradiation, administmtion of chloroform, injection of toxic agents to vegetative nerve system. And a significant positive linear correlation was proved between the serum mucoprotein level and agglutination titer in the animal experiment on the whole. But a dissociation in this relation was observed during anaphylactic shok. Namely serum mucoprotein level tended to fall soon after the reinjection, while the agglutination titer rose higher temporarily and then both showed a tendency to decrease.
Publication Title 岡山大学温泉研究所報告
Published Date 1953-12
Volume volume13
Start Page 24
End Page 30
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40340
language 日本語
File Version publisher
NAID 40017532450
JaLCDOI 10.18926/40329
Title Alternative DIE ERFAHRUNGEN VON GYNEKOLOGISCHEN BALNEOTHERAPIE (5) DER EINFLUSS VON ORALEN ANWENDUNG VON THERMALWASSER AUF DIE LEBERFUNKTION VON SCHWANGEREN FRAU
FullText URL 014_038_044.pdf
Author Hasegawa, Yasumasa|
Abstract Nach oraler Anwendung vom kochsalzhaitigen schwach radioaktiven Thermalwasser von MISASA 500 ccm bei 20 schwangeren Frauen (meistens S. S. IIM. bis S. S. IVM.) wurden die folgneden Untersuchungen über Leberfunktionen angestellt und mit dem Resultat nach oralen Anwendung von Süsswasser verglichen. 1) Das Thermalwasser steigerte die Zusammensetzungsfähigkeit von Hippursäure d. h· Entgiftungsfähigkeit von Leber. 2) Nach Asorbin S Methode erwies sich keine Veränderung. 3) Die Aussheidungsfähigkeit von Bromsulfalein wurde schwach. 4) 4 stundige Harnmenge nach den oralen Anwendung war weniger bei dem Thermalwasser als bei Süsswasser, und NaCl Menge in beiden Harn war gleich. In Rücksicht auf oben genannten Untersuchungen, trotz des speziellen Einflusses auf die Leberfunktion, wegen antidiuretischer Wirkung darf orale Anwendung des Thermalwassers von MISASA bei schwangeren Frau nur mit einer gewissen Vorsicht gemacht werden.
Publication Title 岡山大学温泉研究所報告
Published Date 1954-03
Volume volume14
Start Page 38
End Page 44
ISSN 0369-7142
language 日本語
File Version publisher
NAID 40017532463
JaLCDOI 10.18926/40324
Title Alternative RADON CONTENT OF HOT SPRINGS IN TOTTORI PREFECTURE, JAPAN
FullText URL 014_001_014.pdf
Author Oshima, Yoshio| Yamada, Naoharu| Mifune, Masaaki|
Abstract 1. Radon content of 166 thermal waters in IWAI, TOTTORI YOSHIOKA, HAMAMURA, TOGO, SEKIGANE, MISASA, and KAlKE Hot Springs was measured by I. M. Fontactoscope in the years 1950-1951. Of which 66 samples showed a radon content over 30×10(-10) curie units per liter. Namely, 50 springs in Misasa, 6 in Sekigane, 9 in Hamamura, and one in Togo belonged to the radioactive spring in the definition by Ministry of Social Welfare. The highest Radon content (1150×10(10) curie units per liter) was recorded in Hisuino-Yu in Misasa, where five springs had a radon content over 360×10(-10) curie units per liter. 2. No marked difference was proved between the results obtained this time and the data in the former reports concerning the radon content of these thermal springs. 3. Radon content proved higher in the springs which issue from granite than in the springs of other districts. No definite relation was proved between the radon content and water temperature. The radon content was generally high in simple thermals or in weak sodium chloride springs, low in sulfated springs and in saline springs which had a comparatively high sulfate content.
Publication Title 岡山大学温泉研究所報告
Published Date 1954-03
Volume volume14
Start Page 1
End Page 14
ISSN 0369-7142
language 日本語
File Version publisher
NAID 40017532457
JaLCDOI 10.18926/40322
Title Alternative THE CLINICAL STUDIES OF RHEUMATOID ARTHRITIS (I)
FullText URL 016_035_048.pdf
Author Morinaga, Hiroshi|
Abstract The value of spa treatment of rheumatic disease is an established feature of medical management based on experiences for many centuries. The author has been practising the balneotherapy of rheumatic diseases at Misasa Spa for several years, and has found this therapy efficacious in many cases in relieving the anemia that accompanies rheumatoid arthritis. For this reason, the author is investigating the metabolism of iron in rheumatic anemia, which is expected to occur in the course of the spa treatment. In this report, the results of clinical examinations of 24 patients with rheumatoid arthritis, of the type (2. 2. 2.) Polyarthritis systemica chronica (in Prof. Kodama's classification of arthritis), are described. I. Some observations of anemia in rheumatoid arthritis 1. The peripheral blood The number of erythrocytes is 266×10(4)~455×10(4)/c. mm. (average: (381±32)×10(4)/c. mm.), Hb-content : 55~90% (average : 76±5.8%), and the color index is about 1.0. The anemia of rheumatic disease treated is mostly normochromic. The white blood count is between 3240 and 13300/c. mm. (average: 6421±955/c. mm. ), and the number of the leucocytes in 80% of the cases ranges within the normal limits. Eosinophilia is observed in 15% of the cases. 2. Anemia-producing substance in the serum of rheumatoid arthritis When serum from rheumatoid arthritis patients was injected into rabbits, the number of erythrocytes and the hemoglobin -content of the rabbits was found to decrease markedly for 2~6hours after the injection. But no such effect was observed when the serum of healthy subjects was injected. From this, the author presumes the presence of an anemia-producing substance in the serum of rheumatoid arthritis. The nature of this substance in now under investigation. II. Clinical-biochemical studies 3. Gastric acidity of rheumatic patients Of 7 cases examined, anacidity of gastric juice was observed in 2 cases, subacidity in other 2 cases and normacidity in the remaining 3 cases. Thus, a decrease in the gastric acidity was found to occur in 57% of the cases. 4. Takata-serum-test and sedimentation rate It is said that the sedimentation rate and the Weltmann-reaction are very sensitive indicators of the extent of the activity of rheumatic disease. The Takata-serum-test was applied in 18 cases and was found to give positive results in 10 cases (55%). The sedimentation rate was found to increase in 17 of 20 cases (85%). 5. Blood uric acid As is well known, the concentration of uric acid is abnormally high in the blood of patients with gout; while, according to the literature, the blood uric acid levels of patients with rheumatoid arthritis range within the normal limits. The author, using the method of Benedict, obtained the following results for blood uric acid in 14 normal subjects in fasting state: Range - 2.30~3.47mg./dl. , 5% rejection limit -- 1.97~3.68mg./dl. In 5 of 7 cases of rheumatoid arthritis, the blood uric acid levels were shown to be above the upper limit of the normal range, but the concentrations of blood uric acid of non-rheumatic joint diseases and neuralgias were mostly within the normal range. 6. Blood sugar The fasting blood sugar levels of rheumatoid patients ranged from 82 to 1l0mg./dl. (average : 100mg./dl.), higher than the levels of healthy subjects (78~108mg./dl., average : 90mg./dl.). The intravenously injected glucose load test was applied to 8 rheumatoid patients. A solution of 40 ml. of 20% glucose was injected into a fasting subject for 2 minutes. Blood samples were taken before the injection, 3 minutes after the injection, and at each subsequent 10 minute-interval for 70 minutes. The glucose tolerance curves in 5 of these cases were found to be out of the normal range. It is to be noticed that the average blood sugar for rheumatoid arthritis is similar to the curve for liver disease. 7. Serum alkaline phosphatase activity and serum phosphorus The method of Taussky-Shorr was used for the determination of the level of alkaline phosphatase activity and inorganic phosphate in the serum. The alkaline phosphatase activity of 11 normal individuals ranged from 1.4 to 6.4 Shinowara-Jones-Rheinhart units/dl. (5% rejection limit), while in 4 of 8 cases of rheumatoid arthritis, it was of abnormally high levels. The concentration of inorganic phosphate in the serum of 12 normal subjects ranged from 2.9 to 5.0mg. /dl. (5% rejection limit), while in 3 cases of rheumatoid patients, it showed higher levels than normal. 8. Total cholesterol in serum The total cholesterol in the serum of fasting normal individuals was measured by Bloor's method, and the concentration was found to range from 124 to 188mg./dl. (rejection limit of 5% level of significance), and the total cholesterol in the serum of rheumatoid arthritis to be abnormally high in concentration in 6 of 7 cases. The liver function was evaluated by the Takata-serum-test, the test of fasting blood sugar levels, the glucose load test, and the test of serum alkaline phosphatase activity; and from this the hepatic dysfunction was found to occur in rheumatoid arthritis patients in about 50% of the cases examined. Disturbances in the protein, fat, and carbohydrate metabolism were also found to occur. The above-mentioned findings indicate that rheumatoid arthritis is not only a disease of the joints, but also a general and systemic illness. Attention must accordingly be given to the general condition of the patient in the treatment of rheumatic disease.
Publication Title 岡山大学温泉研究所報告
Published Date 1956-01
Volume volume16
Start Page 35
End Page 48
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40318
language 日本語
File Version publisher
NAID 40017532470
JaLCDOI 10.18926/40319
Title Alternative REPORT OF THE CHEMICAL ANALYSIS OF MISASA HOT SPRINGS, TOTTORI PREFECTURE
FullText URL 016_001_018.pdf
Author Oshima, Yoshio| Mifune, Masaaki| Yamada, Naoharu| Ueyama, Akiko|
Abstract The water samples of 55 springs of Misasa were analysed in the years 1950~1951. The temperatures of the waters ranged from 44° to 85° C. Radon content ranged from 34.1 to 2360×10(-10) curies per liter. pH 6.2~7.5. Evaporation residue: 534~1940 mg/kg. K(+) :10.4~47.3mg/kg. Na(+) : 140.4~574mg/kg. Ca(2+) : 7.56~49.06mg/kg. Mg(2+) : 0.11~15.3mg/kg.. Fe(2+) : 0.10~0.67mg/kg. Cl(-) : 138~854mg/kg. SO(4)(2-) : 29.2~187mg/kg. HCO(3)(-) : 74.8~370mg/kg. HBO(2)(-) : 1.80~19.6mg/kg. S(2)O(3)(2-) : o.62~3.69mg/kg. Radon sources seem to exist at shallow places under the ground and the radon in the thermal wacer is supposed to have been derive partly from the ground water which also contains considerable amount of radon. A close linear correlation (r=0.751) was proved between the sulfate and the chloride ion contents.
Publication Title 岡山大学温泉研究所報告
Published Date 1956-01
Volume volume16
Start Page 1
End Page 18
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40318
language 日本語
File Version publisher
NAID 40017532473
JaLCDOI 10.18926/40316
Title Alternative THREE CASE OF LAMBLIASIS INTESTINALIS
FullText URL 017_033_041.pdf
Author Matsumoto, Kinshi| Nobuoka, Otohiko|
Abstract We experienced three cases of patients with Lambliasis intesinalis recently. and described their clinical features in detail.
Publication Title 岡山大学温泉研究所報告
Published Date 1956-03
Volume volume17
Start Page 33
End Page 41
ISSN 0369-7142
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/40311
language 日本語
File Version publisher
NAID 40017532475