JaLCDOI 10.18926/AMO/52788
FullText URL 68_4_219.pdf
Author Hinamoto, Norikazu| Maeshima, Yohei| Saito, Daisuke| Yamasaki, Hiroko| Tanabe, Katsuyuki| Nasu, Tatsuyo| Watatani, Hiroyuki| Ujike, Haruyo| Kinomura, Masaru| Sugiyama, Hitoshi| Sonoda, Hikaru| Kanomata, Naoki| Sato, Yasufumi| Makino, Hirofumi|
Abstract Experimental studies have demonstrated the involvement of angiogenesis-related factors in the progression of chronic kidney disease (CKD). There have so far been no reports investigating the distribution and clinical roles of Vasohibin-1 (VASH-1), a negative feedback regulator of angiogenesis, in CKD. We recruited 54 Japanese CKD patients and 6 patients who had normal renal tissues excised due to localized renal cell carcinoma. We evaluated the correlations between the renal expression level of VASH-1 and the clinical/histological parameters. VASH-1 was observed in renal endothelial/mesangial cells, crescentic lesions and interstitial inflammatory cells. Significant positive correlations were observed between 1) crescent formation and the number of VASH-1+ cells in the glomerulus (r=0.48, p=0.001) or cortex (r=0.64, p<0.0001), 2) interstitial cell infiltration and the number of VASH-1+ cells in the cortex (r=0.34, p=0.02), 3) the glomerular VEGFR-2+ area and the number of VASH-1+ cells in the glomerulus (r=0.44, p=0.01) or medulla (r=0.63, p=0.01). These results suggest that the renal levels of VASH-1 may be affected by local inflammation, crescentic lesions and VEGFR-2.
Keywords chronic kidney disease inflammation Vasohibin-1 VEGF-A VEGFR-2
Amo Type Original Article
Published Date 2014-08
Publication Title Acta Medica Okayama
Volume volume68
Issue issue4
Publisher Okayama University Medical School
Start Page 219
End Page 233
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2014 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 25145408
Web of Sience KeyUT 000340687500004
Related Url http://ousar.lib.okayama-u.ac.jp/metadata/52824
Author Kitagawa, Masashi| Sugiyama, Hitoshi| Morinaga, Hiroshi| Inoue, Tatsuyuki| Takiue, Keiichi| Ogawa, Ayu| Yamanari, Toshio| Kikumoto, Yoko| Uchida, Haruhito Adam| Kitamura, Shinji| Maeshima, Yohei| Nakamura, Kazufumi| Ito, Hiroshi| Makino, Hirofumi|
Published Date 2013-02-19
Publication Title PLoS ONE
Volume volume8
Issue issue2
Content Type Journal Article
Author Takiue, Keiichi| Sugiyama, Hitoshi| Inoue, Tatsuyuki| Morinaga, Hiroshi| Kikumoto, Yoko| Kitagawa, Masashi| Kitamura, Shinji| Maeshima, Yohei| Wang, Dahong| Masuoka, Noriyoshi| Ogino, Keiki| Makino, Hirofumi|
Published Date 2012-05-25
Publication Title BMC Nephrology
Volume volume13
Issue issue14
Content Type Journal Article
Author Nasu, Tatsuyo| Maeshima, Yohei| Kinomura, Masaru| Hirokoshi-Kawahara, Kumiko| Tanabe, Katsuyuki| Sugiyama, Hitoshi| Sonoda, Hikaru| Sato, Yasufumi| Makino, Hirofumi|
Published Date 2011-04-01
Publication Title 岡山医学会雑誌
Volume volume123
Issue issue1
Content Type Journal Article
JaLCDOI 10.18926/AMO/31820
FullText URL fulltext.pdf
Author Miyake, Kohei| Nishida, Keiichiro| Kadota, Yasutaka| Yamasaki, Hiroko| Nasu, Tatsuyo| Saitou, Daisuke| Tanabe, Katsuyuki| Sonoda, Hikaru| Sato, Yasufumi| Maeshima, Yohei| Makino, Hirofumi|
Abstract <p>Angiogenesis is an essential event in the development of synovial inflammation in rheumatoid arthritis (RA). The aim of the current study was to investigate the expression of vasohibin-1, a novel endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor, in the RA synovium, and to test the effect of inflammatory cytokines on the expression of vasohibin-1 by RA synovial fibroblasts (RASFs). Synovial tissue samples were obtained at surgery from patients with osteoarthritis (OA) and RA, and subjected to immunohistochemistry to investigate the expression and distribution of vasohibin-1 relevant to the degree of synovial inflammation. In an in vitro analysis, RASFs were used to examine the expression of vasohibin-1 and VEGF mRNA by real-time PCR after stimulation with VEGF or inflammatory cytokines under normoxic or hypoxic conditions. The immunohistochemical results showed that vasohibin-1 was expressed in synovial lining cells, endothelial cells, and synovial fibroblasts. In synovial tissue, there was a significant correlation between the expression of vasohibin-1 and histological inflammation score (p0.002, r0.842). In vitro, stimulation with VEGF induced the expression of vasohibin-1 mRNA in RASFs under normoxic conditions, and stimulation with cytokines induced vasohibin-1 mRNA expression under a hypoxic condition. These results suggest that vasohibin-1 was expressed in RA synovial tissue and might be regulated by inflammatory cytokines.</p>
Keywords angiogenesis vasohibin-1 rheumatoid arthritis synovial membrane VEGF
Amo Type Original Article
Published Date 2009-12
Publication Title Acta Medica Okayama
Volume volume63
Issue issue6
Publisher Okayama University Medical School
Start Page 349
End Page 358
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20035291
Web of Sience KeyUT 000273145900006
Author 前島 洋平|
Published Date 1997-03-25
Publication Title
Content Type Thesis or Dissertation