Faculty of Engineering, Okayama University Acta Medica Okayama 0475-0071 36 1 2001 Improvement of Biocompatibility of Silicone Elastomer by Surface Modification 117 121 EN Yuki Shirosaki Kanji Tsuru Satoshi Hayakawa Akiyoshi Osaka Seisuke Takashima γ-Methacryloxypropyltrimethoxysilane (γ-MPS) was grafted to silicone due to emulsion polymerization to induce Si-OH groups, in order to provide silicone with bioactivity spontaneous deposition of apatite in body fluid and to improve cytocompatibility. Apatite deposited on the grafted silicone within 7 days of soaking in 1.5 times as concentrated as the Kokubo solution. Osteoblastic cells (MC3T3-E1) were cultured on the specimens up to 7 days. After 5 days of culture, the number of MC3T3-E1 cells on the grafted specimen was much greater than that on the original specimen. These results indicated that the biocompatibility of silicone elastomer was improved by the grafting γ-MPS. No potential conflict of interest relevant to this article was reported.

Faculty of Engineering, Okayama University Acta Medica Okayama 0475-0071 34 1-2 2000 Cytocompatibility of Silicone Elastomer Treated with Hydrogenperoxide Containing Tantalum Chloride 39 43 EN Satoshi Hayakawa Yuki Shirosaki Takeshi Yabuta Kanji Tsuru Akiyoshi Osaka Silicone elastomer was chemically treated at 60℃ for 7 days with 30 wt% H(2)O(2) solutions with or without TaCl(5) and soaked for various periods in a simulated body fluid(Kokubo solution) up to 21 days. Apatite formation ability of the surface of the silicone elastomer specimens was investigated with thin-film X-ray diffraction and FT-IR reflection spectroscopy. These silicone specimens did not deposit apatite or calcium phosphates, irrespective of chemical treatment. Osteoblast-like cells (MC3T3-El) derived from mouse were cultured on the specimens at 36.5℃ under 5%C0(2) and 95% humidity. Similar degree of proliferation of cells was observed at 7 days among three specimens, while the no treatment specimen after incubation for 5 days showed a lower degree of proliferation than the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). Alkaline phosphatase activity of the cells proliferated on the no treatment specimen was lower than those of the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). These results indicate that the cytotoxicity of the silicone could be improved by the chemical treatment with 30 wt% H(2)O(2) solutions with or without TaCl(5). No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2005 Synthesis of two- and three dimensional organic-inorganic hybrids for inducing biomineralization EN Yuki Shirosaki No potential conflict of interest relevant to this article was reported.