Author Bekku, Yoko| Ninomiya, Yoshifumi| Oohashi, Toshitaka|
Published Date 2012-04-01
Publication Title 岡山医学会雑誌
Volume volume124
Issue issue1
Content Type Journal Article
Author Demircan, Kadir| Hirohata, Satoshi| Nishida, Keiichiro| Hatipoglu, Omer F.| Oohashi, Toshitaka| Yonezawa, Tomoko| Apte, Suneel S.| Ninomiya, Yoshifumi|
Published Date 2005-5
Publication Title Arthritis & Rheumatism
Volume volume52
Issue issue5
Content Type Journal Article
JaLCDOI 10.18926/AMO/31831
FullText URL fulltext.pdf
Author Hatipoglu, Omer Faruk| Hirohata, Satoshi| Yaykasli, Kursat Oguz| Cilek, Mehmet Zeynel| Demircan, Kadir| Shinohata, Ryoko| Yonezawa, Tomoko| Oohashi, Toshitaka| Kusachi, Shozo| Ninomiya, Yoshifumi|
Abstract <p>ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatory-induced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA.</p>
Keywords ADAMTS1 gene regulation metalloproteinase
Amo Type Original Article
Published Date 2009-04
Publication Title Acta Medica Okayama
Volume volume63
Issue issue2
Publisher Okayama University Medical School
Start Page 79
End Page 85
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19404339
Web of Sience KeyUT 000265457600002
JaLCDOI 10.18926/AMO/31728
FullText URL fulltext.pdf
Author Nomoto, Hiroyuki| Oohashi, Toshitaka| Hirakawa, Satoshi| Ueki, Yasuyoshi| Ohtsuki, Hiroshi| Ninomiya, Yoshifumi|
Abstract <p>We herein determined by fluorescence in situ hybridization the chromosomal localization of 2 human genes, BRAL1 and BCAN, both of which belong to the link-module superfamily, i.e. to the same band of chromosome 1q21-23. Further analysis of the genomic organization of BRAL1 and BCAN revealed that the BRAL1 gene was located 20-kb upstream of the BCAN start site. We isolated a polymorphic dinucleotide (CA) repeat sequence from a genomic clone containing the BCAN gene. High heterozygosity (0.79) makes this polymorphism a useful marker in the study of genetic disorders. Knowledge of the structure of the genes and the marker provides essential information for further analysis of the gene locus at chromosome 1q21-23.</p>
Keywords BRAL1 BCAN FISH schizophrenia polymorphic marker
Amo Type Article
Published Date 2002-02
Publication Title Acta Medica Okayama
Volume volume56
Issue issue1
Publisher Okayama University Medical School
Start Page 25
End Page 29
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11873941
Web of Sience KeyUT 000174031300005
Author 大橋 俊孝|
Published Date 1992-03-28
Publication Title
Content Type Thesis or Dissertation