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ID 15878
Eprint ID
15878
FullText URL
Thumnail 100_177.pdf 4.16 MB
Title Alternative
Three dimensional architecture of the rat femur bone marrow stroma : A scanning electron microscope study combined with fixation and post-ultrasonication method
Author
Song, Zheng-Ian
Kikuta, Akio
Ohtani, Osamu
Murakami, Takuro
Sano, Tadashi
Abstract
The three dimensional architecture of the rat femur bone marrow stroma was observed by scanning electron microscopy. The bone marrow was fixed and vibrated by ultrasonication to dislodge the hematopoietic cells embedded in the meshes of the marrow reticulum. The stromal reticulum was exposed, while its three dimensional architecture was preserved.The marrow stromal reticulum consisted of intersinusoidal reticular cells and perivascular reticular cells (adventitial cells), and a small amount of reticular fibers. Two types of meshwork were noted in the bone marrow reticulum. Each mesh of one type was the size of one hematopoietic cell. This meshwork consisted of short and broad or stout cytoplasmic processes. A hematopoietic cell was enwrapped by attenuated cytoplasmic sheaths of the reticular cells and sequestered from the surrounding hematopietic cells. The other type of meshwork was composed of straight running, long and slender filopodia of the reticular cells. Several hematopoietic cells were tightly packed in each mesh of the meshwork of this type, thus presenting a polyhedral appearance. Attenuated adventitial cells enveloped the marrow blood vessels, and were connected with each other by their sheath-like cytoplasmic processes but round, variously sized intercellular spaces remained. The intersinusoidal reticular cells extended their processes to the adventitial cells and the adventitial cells also stretched their processes to the intersiusoidal reticulum.
Keywords
bone marrow stroma
rat
reticulum
ultrasonication scanning electron microscopy
Published Date
1988
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume100
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
177
End Page
185
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/100/1-2/100_1-2_177/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma