JaLCDOI 10.18926/AMO/32865
FullText URL fulltext.pdf
Author Murai, Hiroyasu| Hiragami, Fukumi| Kawamura, Kenji| Motoda, Hirotoshi| Koike, Yoshihisa| Inoue, Shigeki| Kumagishi, Kanae| Ohtsuka, Aiji| Kano, Yoshio|
Abstract <p>Among the 3 mitogen-activated protein kinases -- ERK, p38 MAPK and JNK -- JNK has been suggested to participate in apoptosis, whereas p38 MAPK is thought to be part of the differentiation response. There are many common inducers of JNK and p38 MAPK, but the mechanisms underlying the differential response to apoptosis and differentiation are poorly understood. We found that heatshock activated p38 MAPK at 3min after exposure to a temperature of 44 in stress-hypersensitive PC12m3 mutant cells, while it activated JNK at 20min after the same heat treatment. However, heat shock activated p38 MAPK 5min after heat treatment and JNK 10min after heat treatment in PC12 parental cells. The extent of phosphorylation of p38 MAPK induced by heat shock in PC12m3 cells was significantly greater than that in PC12 parental cells, and a high level of heat-shock-induced neurite outgrowth was observed only in PC12m3 cells. On the other hand, heat-shock-induced JNK activation appeared more quickly and apoptosis started earlier in PC12 parental cells. These findings indicate that short stress induces p38 MAPK and longer stress induces JNK, and that the response of these kinases to heat shock differs depending on cell type.</p>
Keywords heat shock neurite outgrowth p38 MAP kinase JNK PC12 mutant cells
Amo Type Original Article
Published Date 2010-02
Publication Title Acta Medica Okayama
Volume volume64
Issue issue1
Publisher Okayama University Medical School
Start Page 55
End Page 62
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20200585
Web of Sience KeyUT 000274868300008
JaLCDOI 10.18926/AMO/40502
FullText URL 64_5_277.pdf
Author Kumase, Fumiaki| Morizane, Yuki| Mohri, Satoshi| Takasu, Ippei| Ohtsuka, Aiji| Ohtsuki, Hiroshi|
Abstract Endothelial glycocalyx (GCX) has been reported as a protective factor for vascular endothelial cells (VEC) in diabetes and hypertension. However, the involvement of GCX impairment in ocular vasculopathy remains unclear. We evaluated the changes in the GCX thicknesses of the retinal and choroidal capillaries in rats with diabetes and hypertension by cationic colloidal iron staining using a transmission electron microscope. In the control group, the mean (standard error of the mean) thicknesses of retinal and choroidal GCX were 60.2 (1.5) nm and 84.3 (3.1) nm, respectively. The diabetic rats showed a significant decrease of GCX thickness in the retina, but not in the choroid, compared to controls (28.3 (0.3) nm, p<0.01 and 77.8 (1.4) nm, respectively). In the hypertensive rats, both retinal and choroidal GCX were significantly decreased compared to the control values (10.9 (0.4) nm and 13.2 (1.0) nm, respectively, both p<0.01). Moreover, we could visualize the adhesion of leukocytes and platelets on the luminal surface of VEC, at the site where the GCX was markedly degraded. These findings suggest that the GCX prevents adhesion of leukocytes and platelets to the VEC surface, and this impairment may lead to ocular vasculopathy in diabetes and hypertension.
Keywords glycocalyx retina choroid diabetes hypertension
Amo Type Original Article
Published Date 2010-10
Publication Title Acta Medica Okayama
Volume volume64
Issue issue5
Publisher Okayama University Medical School
Start Page 277
End Page 283
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2010 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 20975760
Web of Sience KeyUT 000283563300002
JaLCDOI 10.18926/AMO/31855
FullText URL fulltext.pdf
Author Nishibori, Masahiro| Takahashi, Hide K.| Katayama, Hiroshi| Mori, Shuji| Saito, Shinya| Iwagaki, Hiromi| Tanaka, Noriaki| Morita, Kiyoshi| Ohtsuka, Aiji|
Abstract Lipopolysaccharide (LPS) is one of the major causes of septic shock. The polymyxin B-immobilized filter column (PMX) was developed for the adsorption of endotoxin by direct hemoperfusion and has been used for the treatment of LPS-induced septic shock. In this study, we demonstrated that PMX also specifically bound monocytes from the peripheral blood leukocytes of septic patients by mean of an analysis of bound cells using immunocytochemical and electron microscopic techniques. The specific removal of monocytes from septic patients may produce beneficial effects by reducing the interaction between monocytes and functionally associated cells including vascular endothelial cells.
Keywords septic shock polymixin B-immobilized column monocyte adsorptive removal
Amo Type Short Communication
Published Date 2009-02
Publication Title Acta Medica Okayama
Volume volume63
Issue issue1
Publisher Okayama University Medical School
Start Page 65
End Page 69
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
Web of Sience KeyUT 000263730300009
JaLCDOI 10.18926/AMO/30753
FullText URL fulltext.pdf
Author Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji|
Abstract <p>We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.</p>
Keywords colloidal iron microwave histochemistry transmission electron microscopy
Amo Type Article
Published Date 2006-02
Publication Title Acta Medica Okayama
Volume volume60
Issue issue1
Publisher Okayama University Medical School
Start Page 59
End Page 64
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16508690
Web of Sience KeyUT 000235538900007
JaLCDOI 10.18926/AMO/31710
FullText URL fulltext.pdf
Author Murakami, Shinichiro| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>Two previously unknown anomalies of the anterior intercostobrachial nerve were described. In one case, the anterior intercostobrachial nerve penetrated the pectoralis minor muscle. In the other case, it penetrated the pectoralis major muscle. In both cases, the anomalous nerve supplied the skin of the upper arm.</p>
Keywords anomalies of intercostobrachial nerve lateral cutaneous branch of the second intercostal nerve pectoralis minor muscle pectoralis major muscle
Amo Type Article
Published Date 2002-10
Publication Title Acta Medica Okayama
Volume volume56
Issue issue5
Publisher Okayama University Medical School
Start Page 267
End Page 269
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12530511
Web of Sience KeyUT 000178668100008
JaLCDOI 10.18926/AMO/31711
FullText URL fulltext.pdf
Author Kosaka, Motohiro| Horiuchi, Kanji| Nishida, Keiichiro| Taguchi, Takehito| Murakami, Takuro| Ohtsuka, Aiji|
Abstract <p>The celiac and mesenteric arterial system including the left gastric, splenic, common hepatic, and superior mesenteric arteries shows various types of origins, courses, ramifications and anastomoses. In order to explain the various expressions of this system, we have proposed a typological model, in which celiacomesenteric arteries develop as paired or bilaterally symmetrical primordial vessels originated from the anterior aspect of the aorta, and these vessels anastomose each other with longitudinal and horizontal pathways. Here, we report 3 unusual cases characterized by arterial rings, formed by the left gastric, left accessory hepatic, proper hepatic, anterior pancreaticoduodenal, and dorsal pancreatic arteries. The dorsal pancreatic and anterior pancreaticoduodenal arteries are located to the right and left of the embryonic pancreas developing in the dorsal mesentery, respectively. Such hepatopancreatic arterial rings simultaneously containing right and left elements can only be explained using our typological model, in which the concept of paired arteries or bilateral symmetry is introduced.</p>
Keywords arterial variation celiac trunk superior mesenteric artery typology bilateral symmetry
Amo Type Article
Published Date 2002-10
Publication Title Acta Medica Okayama
Volume volume56
Issue issue5
Publisher Okayama University Medical School
Start Page 245
End Page 253
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12530508
Web of Sience KeyUT 000178668100005
JaLCDOI 10.18926/AMO/32280
FullText URL fulltext.pdf
Author Su, Wei-Dong| Ohtsuka, Aiji| Taguchi, Takehito| Murakami, Takuro|
Abstract <p>The accessory ascending cervical artery (Murakami et al., 1996), which arises from the subclavian artery and ascends between the scalenus anterior and medius muscles, was studied in 87 Japanese adult cadavers (174 sides), with special attention being given to its origin, distribution, and relationship to other arteries at the cervical or scalenus region. In 154 sides (88.5%), the accessory ascending cervical artery was found to originate from the subclavian artery behind the scalenus anterior muscle, and to branch out to the scalenus anterior and medius muscles as well as those entering the 5th and 6th intervertebral foramens along the 6th and 7th cervical nerves. This artery arose independently in 105 sides. The accessory ascending cervical artery issued off or formed a common trunk with the transverse cervical artery and/or costocervical trunk in 49 sides. In cases lacking the accessory ascending cervical artery, it was usually compensated for by the costocervial trunk and/or transverse cervical artery (18 sides). Common trunk formation with the vertebral, internal thoracic, or suprascapular arteries was not observed. The authors suggest that the accessory ascending cervical artery, the transverse cervical artery, and the costocervical trunk should be grouped into one arterial system, a system that may be a remnant of the precostal longitudinal anastomoses of intersegmental arteries of the dorsal aorta behind the scalenus anterior muscle.</p>
Keywords accessory ascending cervical artery transverse cervical artery costocervical trunk scalenus anterior muscle subclavian artery
Amo Type Article
Published Date 2000-12
Publication Title Acta Medica Okayama
Volume volume54
Issue issue6
Publisher Okayama University Medical School
Start Page 243
End Page 252
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11132917
Web of Sience KeyUT 000166042900002
JaLCDOI 10.18926/AMO/32837
FullText URL fulltext.pdf
Author Murakami, Shinichiro| Horiuchi, Kanji| Yamamoto, Chugo| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>A rare anomaly of the scalenus muscles is described. In this case, the right scalenus anterior muscle was absent. As a substitute for this muscle, some aberrant muscle slips arose from the lower vertebrae and descended in front of the ventral rami of the lower cervical nerves. These aberrant slips then ran between the ventral rami of the the eighth cervical and first thoracic nerves, and were fused with the right scalenus medius muscle. Thus, the subclavian artery and vein ran in front of the aberrant slips, together with the ventral ramus of the first thoracic nerve. The aberrant muscle slips issued 2 accessory bundles. One bundle ran between the ventral rami of the fourth and fifth cervical nerves and was fused with the scalenus medius muscle; the other bundle ran between the ventral rami of the fifth and sixth cervical nerves and was fused with the scalenus medius muscle.</p>
Keywords scalenus anterior muscle scalenus medius muscle ventral rami of the lower cervical nerves ventral ramus of the first thoracic nerve subclavian artery and vein
Amo Type Article
Published Date 2003-06
Publication Title Acta Medica Okayama
Volume volume57
Issue issue3
Publisher Okayama University Medical School
Start Page 159
End Page 161
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 12908014
Web of Sience KeyUT 000183816500008
JaLCDOI 10.18926/AMO/30773
FullText URL fulltext.pdf
Author Hitomi, Sayoko| Su, Wei Dong| Hong, Luo Jia| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>Sections of the visual cortex of newborn (1-4 weeks after birth) and adult cats were stained with cationic iron colloid, aldehyde fuchsin or lectins (lectin Vicia villosa, soybean and Wisteria floribunda agglutinins). Many neurons in the adult cat visual cortex contained perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectins. Double staining indicated that some of the lectin-labeled neurons were not stained with cationic iron colloid, and also that some of the cationic iron colloid-stained neurons were not labeled with lectins. The perineuronal sulfated proteoglycans and cell surface glycoproteins developed 3 weeks after birth. In the newborn cats 1-2 weeks after birth, no neurons were reactive to cationic iron colloid, aldehyde fuchsin or lectins. In the newborn cats 34 weeks after birth, it was clearly observed that the cytoplasm of the glial cells closely associated with the neurons containing the perineuronal sulfated proteoglycans showed an intense reaction to cationic iron colloid and aldehyde fuchsin, and that the Golgi complexes of the neurons with cell surface glycoproteins were intensely labeled with lectins. These findings suggest that the perineuronal sulfated proteoglycans are derived from the associated glial cells, and that the cell surface glycoproteins are produced by the associated nerve cells.</p>
Keywords cat brain perineuronal sulfated proteoglycans cell surface glycoproteins cationic iron colloid aldehyde fuchsin lectin
Amo Type Article
Published Date 1997-12
Publication Title Acta Medica Okayama
Volume volume51
Issue issue6
Publisher Okayama University Medical School
Start Page 295
End Page 299
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9439770
Web of Sience KeyUT 000071183400001
JaLCDOI 10.18926/AMO/31299
FullText URL fulltext.pdf
Author Piao, Da Xun| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>Origins and distribution of the human inferior phrenic arteries were studied by dissecting 68 Japanese adult cadavers. The inferior phrenic arteries were usually observed as paired (left and right) vessels. Their origins were summarized as follows: a) the aorta itself (85/138 cases, 61.6%), b) the ventro-visceral arteries (celiaco-mesenteric system of the aorta) including the celiac trunk (39/138 cases, 28.2%) and the left gastric artery (4/138 cases, 2.9%), and c) the latero-visceral arteries (adreno-renal system of the aorta) including the middle adrenal artery (4/138 cases, 2.9%) and the renal artery (6/138 cases, 4.3%). The left and right arteries occasionally originated in common trunk from the aorta, celiaco-mesenteric system or adreno-renal system (22/138 cases, 15.9%). A typological diagram explaining these variations is given. The inferior phrenic arteries, especially the left ones, sometimes issued visceral or esophageal branches. This fact indicates that the inferior phrenic arteries are homologous with the celiac trunk and mesenteric arteries. It is further discussed that the celiac trunk and mesenteric arteries are originally paired vessels, through introduction of our previous typological diagram of the abdominal arteries.</p>
Keywords inferior phrenic artery esophageal branch typology of abdominal arteries
Amo Type Article
Published Date 1998-08
Publication Title Acta Medica Okayama
Volume volume52
Issue issue4
Publisher Okayama University Medical School
Start Page 189
End Page 196
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9781269
Web of Sience KeyUT 000075623600003
JaLCDOI 10.18926/AMO/31320
FullText URL fulltext.pdf
Author Murakami, Takuro| Mabuchi, Masaru| Giuvarasteanu, Lleana| Kikuta, Akio| Ohtsuka, Aiji|
Abstract <p>Some rare anomalies of the celiaco-mesenteric system were observed postmortem in a Japanese adult male: a) The left gastric, common hepatic, splenic and superior mesenteric arteries arose independently from the abdominal aorta. b) The anterior inferior pancreaticoduodenal artery of the superior mesenteric artery issued a hepatic artery which ascended along the anterior surface of the pancreas and gave off the right gastroepiploic, right gastric and cystic arteries. c) The common hepatic artery gave off an anastomosing branch to the superior mesenteric artery. d) The left gastric artery gave off the left accessory hepatic artery. e) The splenic artery issued the accessory middle colic artery. f) The left inferior phrenic artery gave off the esophageal branch. These anomalies are discussed in light of a typological system which we proposed in a previous paper for the celiaco-mesenteric system.</p>
Keywords arterial anomaly celiac trunk left gastric artery hepatic artery splenic artery intermesenteric anastomosis
Amo Type Article
Published Date 1998-10
Publication Title Acta Medica Okayama
Volume volume52
Issue issue5
Publisher Okayama University Medical School
Start Page 239
End Page 244
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9810433
Web of Sience KeyUT 000076694300002
JaLCDOI 10.18926/AMO/31339
FullText URL fulltext.pdf
Author Pu, Jian| Nishida, Keiichiro| Inoue, Hajime| Asahara, Hiroshi| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>Mast cells in osteoarthritic and rheumatoid arthritic synovial tissues of the human knee.</p>
Keywords mast cell count osteoaethritis rheumatoid arthritis hydroarthrosis synovial membrance
Amo Type Article
Published Date 1998-02
Publication Title Acta Medica Okayama
Volume volume52
Issue issue1
Publisher Okayama University Medical School
Start Page 35
End Page 39
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 9548992
Web of Sience KeyUT 000072264100005
JaLCDOI 10.18926/AMO/32301
FullText URL fulltext.pdf
Author Endo, Ryutaro| Murakami, Shinichiro| Masuda, Yu| Taguchi, Takehito| Ohtsuka, Aiji| Nishizaki, Kazunori| Murakami, Takuro|
Abstract <p>The present study showed that many neurons in the adult rat brain possessed a perineuronal sulfated proteoglycan surface coat which reacted to cationic iron colloid and aldehyde fuchsin. This surface coat was stained supravitally with Ehrlich's methylene blue and doubly stained with Ehrlich's methylene blue and aldehyde fuchsin. The surface coat was also stained with Gomori's ammoniacal silver and doubly stained with Gomori's ammoniacal silver and cationic iron colloid. The surface coat was usually expressed together with a nerve cell surface glycoprotein net detectable with lectin Wisteria floribunda agglutinin. These findings indicate that the perineuronal proteoglycan surface coat is identical to Cajal's superficial reticulum and contains some collagenous elements. It was further demonstrated that collagenase digestion erased Gomori's ammoniacal silver impregnation within the perineuronal proteoglycan surface coat.</p>
Keywords brain extracellular matrix perineuronal proteoglycans cell surface glycoproteins
Amo Type Article
Published Date 2000-06
Publication Title Acta Medica Okayama
Volume volume54
Issue issue3
Publisher Okayama University Medical School
Start Page 111
End Page 118
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10925735
Web of Sience KeyUT 000087965700003
JaLCDOI 10.18926/AMO/30373
FullText URL fulltext.pdf
Author Okada, Satoko| Ohtsuka, Aiji| Akagi, Hirofumi| Nishizaki, Kazunori| Masuda, Yu|
Abstract <p>It has previously been confirmed that the guinea pig has aggregations of 10-20 lymphoid follicles at the junction of the nasal cavity and the nasopharyngeal duct. The vascular architecture of this nasal-associated lymphoid tissue (NALT) was studied by the corrosion cast/scanning electron microscope method. The NALT was supplied by branches of the inferior nasal artery. These afferent arterial branches gave off arterioles to the follicles and the interfollicular regions, where the arterioles ramified into capillaries. Some of these arterioles reached the subepithelial region to form a single-layer dense capillary network. The subepithelial capillaries gathered into short collecting venules, which in turn drained into high endothelial venules (HEV) in the interfollicular region. The HEV, which also receives tributaries from the follicular and interfollicular capillary plexuses, descended in the interfollicular regions and finally flowed into the efferent veins at the bottom of the NALT. Indentations impressed by high endothelial cells (HEC) were prominent on the surface of the HEV casts, and their frequency was larger in the upper course or segments than in the lower. This suggests that the incidence of HEC in the upper segments is higher than in the lower segments, and these findings are consistent with the hypothesis that some substances which are taken up into the subepithelial capillaries and transported to the venules induce differentiation and maintain of HEVs.</p>
Keywords nasal-associated lymphoid tisse vascular corrosion cast microvascular architecture high endothelial venule guinea pig
Amo Type Article
Published Date 1995-08
Publication Title Acta Medica Okayama
Volume volume49
Issue issue4
Publisher Okayama University Medical School
Start Page 213
End Page 219
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7502682
Web of Sience KeyUT A1995RR97800006
JaLCDOI 10.18926/AMO/30483
FullText URL fulltext.pdf
Author Tsubouchi, Mari| Tsubochi, Yutaka| Hitomi, Sayoko| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>Many neurons in the adult rat cingulate cortex possess perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectin Vicia villosa or soybean agglutinin. The perineuronal sulfated proteoglycans develop three to four weeks after birth. The cell surface glycoproteins develop at earlier stage or two to three weeks after birth. Dark or active neurons begin to appear three to four weeks after birth. These findings indicate that the brain matures after birth or during weaning period.</p>
Keywords rat brain perineuronal sulfated proteoglycans cell surface glycoproteins dark neurons
Amo Type Article
Published Date 1996-12
Publication Title Acta Medica Okayama
Volume volume50
Issue issue6
Publisher Okayama University Medical School
Start Page 313
End Page 317
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8985468
Web of Sience KeyUT A1996WA04500005
JaLCDOI 10.18926/AMO/30495
FullText URL fulltext.pdf
Author Tsubouchi, Yutaka| Tsubouchi, Mari| Hitomi, Sayoko| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>Neurons of cerebellar nuclei in the rat brain had a marked surface coat which was stained with cationic iron colloid or aldehyde fuchsin. Neurons with a similar surface coat were also noted in the retrosplenial cortex. The surface coat was stained doubly with cationic iron colloid and aldehyde fuchsin. Digestion with hyaluronidase eliminated the stainability of the surface coat to both agents. Combined digestion with chondroitinase ABC, heparitinase and keratanase eliminated the cationic iron colloid staining but did not interfere with the aldehyde fuchsin staining. Electron microscopy of ultrathin sections revealed that the iron particles were deposited in the perineuronal tissue spaces. These findings indicate that the surface coat consists of sulfated proteoglycans which occupy, as the extracellular matrix, the perineuronal tissue spaces. Many neurons in the retrosplenial cortex were labeled with lectin Vicia villosa agglutinin. Double staining revealed that these lectin-labeled neurons are usually reactive to cationic iron colloid. Few neurons in the cerebellar nuclei were labeled with lectin V. villosa agglutinin.</p>
Keywords perineuronal sulfated proteoglycans cationic iron colloid staining aldehyde fuchsin staining lectin VVA labeling rat brain
Amo Type Article
Published Date 1996-10
Publication Title Acta Medica Okayama
Volume volume50
Issue issue5
Publisher Okayama University Medical School
Start Page 237
End Page 241
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8914676
Web of Sience KeyUT A1996VQ20600002
JaLCDOI 10.18926/AMO/30497
FullText URL fulltext.pdf
Author Tanaka, Toshihisa| Tsubouchi, Mari| Tsubouchi, Yutaka| Ohtsuka, Aiji| Murakami, Takuro|
Abstract <p>The blood vascular bed, perivascular space and intercellular space of the rat parathyroid gland were studied using scanning electron microscopy of vascular casts, freeze-cracked tissue samples, and NaOH-digested tissue blocks. The findings were supplemented by transmission light and electron microscopy of iron colloid-treated or enzyme-digested tissue sections. The rat parathyroid gland contained a rich network of capillaries. These capillaries were surrounded by marked pericapillary spaces which were demarcated by basal lamina of both capillaries and parenchymal cells. The pericapillary spaces contained numerous collagen fibrils, and issued many crista-like projections which ran deep into the sheets of parenchymal cells. The intercellular spaces of parenchymal cells contained neither basal lamina nor collagen fibrils. The surfaces of the parenchymal cells showed strong negative charging, and maintained the intercellular spaces. The luminal surfaces of the capillary endothelium also showed strong negative charging, and maintained the capillary lumen.</p>
Keywords parathyroid gland cationic and anionic iron colloid stainings vascular casting freeze fracture maceration
Amo Type Article
Published Date 1996-10
Publication Title Acta Medica Okayama
Volume volume50
Issue issue5
Publisher Okayama University Medical School
Start Page 242
End Page 253
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8914677
Web of Sience KeyUT A1996VQ20600003
JaLCDOI 10.18926/AMO/31092
FullText URL fulltext.pdf
Author Murakami, Takuro| Tsubouchi, Mari| Tubouchi, Yutaka| Taguchi, Takehito| Ohtsuka, Aiji|
Abstract <p>Neurons with strongly negatively charged surface coats were recognized in mammalian, avian, reptilian, amphibian and piscine brains. Many large-sized neurons had strongly negatively charged surface coats in the visual cortex and brain stem of the cow, cat, guinea pig, mouse, quail and parakeet. Such neurons were also seen in the brain stem of the lower vertebrates such as the house lizard, Japanese terrapin, bullfrog, newt, carp and sweetfish.</p>
Keywords central nervous system neurons negatively charged surface coats proteoglycans
Amo Type Article
Published Date 1994-08
Publication Title Acta Medica Okayama
Volume volume48
Issue issue4
Publisher Okayama University Medical School
Start Page 195
End Page 197
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7817774
Web of Sience KeyUT A1994PE51400004
JaLCDOI 10.18926/AMO/31000
FullText URL fulltext.pdf
Author Yamamoto, Chugo| Murakami, Takuro| Ohtsuka, Aiji|
Abstract <p>The deep palmar muscles in monkey hands were studied. The contrahentes muscles mainly arose from the capitate bone, descended palmar to the deep palmar branch of the ulnar nerve and the palmar metacarpophalangeal nerves, and attached to the proximal phalanges or wing tendons of the second, fourth and fifth fingers. In relation to the deep palmar branch of the ulnar nerve and the palmar metacarpophalangeal nerves, the contrahentes muscles are homologous with the adductor pollicis and flexor indicis radialis muscles. The contrahentes muscles occasionally gave off some accessory slips which blended with the interosseous muscles. These findings suggest that the human adductor pollicis muscle is a well-developed remnant of a contrahens muscle, and that the human interosseous muscles contain some remnant of the contrahentes muscle. In fact, a well-developed remnant of a contrahens muscle was found in the fourth finger of a human hand. It is further considered that the human adductor pollicis muscle contains an element of the interosseous muscle of the thumb.</p>
Keywords monkey hands contrahentes muscles adductor pollicis muscle flexor indicis radialis musle interosseous muscles
Amo Type Article
Published Date 1988-08
Publication Title Acta Medica Okayama
Volume volume42
Issue issue4
Publisher Okayama University Medical School
Start Page 215
End Page 226
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3177007
Web of Sience KeyUT A1988P884600005
JaLCDOI 10.18926/AMO/31005
FullText URL fulltext.pdf
Author Ikebuchi, Yoshifumi| Murakami, Takuro| Ohtsuka, Aiji|
Abstract <p>The interosseous and lumbrical muscles in twenty-five hands of Japanese adult cadavers were dissected. The palmar and dorsal interosseous muscles continued, with few exceptions, into the wing tendons. The dorsal interosseous muscles gave off tendons which pierced the transverse laminae or passed deep to the transverse laminae, and attached to the bases of the proximal phalanges. The palmar interosseous muscles seldom had such attachments. The palmar and dorsal interosseous muscles sometimes gave off additional tendons which passed superficial to the transverse laminae and attached to the bases of the proximal phalanges. These latter attachments were typical in the contrahentes muscles. Thus, the present findings suggest that the human dorsal interosseous muscles are composite muscles derived from the dorsal abductor, flexor brevis and contrahens muscles, and that the human palmar interosseous muscles are composite muscles derived from the flexor brevis and contrahens muscles. The lumbrical muscles rarely gave off accessory slips with atavistic attachments to the proximal phalanges.</p>
Keywords human hand palmar interosseous muscles dorsal interosseous muslcles lumbrical muscles
Amo Type Article
Published Date 1988-12
Publication Title Acta Medica Okayama
Volume volume42
Issue issue6
Publisher Okayama University Medical School
Start Page 327
End Page 334
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 3239437
Web of Sience KeyUT A1988R743300004