Author Inada, Yoshihiko| Kakihara, Seiji| Kita, Masakazu| Yamashita, Nobuhiko| Ishikawa, Teruhiko| Ogura, Hisakazu| Ando, Motonori| Kusachi, Isao| Kato, Kuranoshin| Yoshioka, Tsutomu| Kawata, Yuki| Hirakawa, Motoshi| Hashimoto, Seiji| Akazaki, Tetsuya| Higashi, Nobuhiko| Becchaku, Akio|
Published Date 2007-03-10
Publication Title 岡山大学教育実践総合センター紀要
Volume volume7
Issue issue1
Content Type Departmental Bulletin Paper
Author Inada, Yoshihiko| Irie, Izumi| Atobe, Kozo| Ando, Motonori| Ishikawa, Teruhiko| Uno, Koji| Ogura, Hisakazu| Kakihara, Seiji| Kato, Kuranoshin| Kita, Masakazu| Tanaka, Kenji|
Published Date 2009-03-10
Publication Title 岡山大学教育実践総合センター紀要
Volume volume9
Issue issue1
Content Type Departmental Bulletin Paper
Author Yamaji, Mayumi| Inoue, Risa| Edamatsu, Midori| Ando, Motonori|
Published Date 2015-04
Publication Title 岡山実験動物研究会報
Volume volume31
Content Type Journal Article
Title Alternative Structure and function of tegmentum vasculosum in avian cochlea
FullText URL poalas_033_026_030.pdf
Author Ikeda, Risa| Otono, Tsuyoshi| Ikeda, Naoya| Saito, Noboru| Ando, Motonori|
Abstract In spite of the importance of endocochlear DC potential (EP) and the K+-rich endolymph for the avian cochlea, the structure and function of the tegmentum vasculosum (TV) has not yet been fully elucidated, compared with those of the stria vascularis in the mammalian cochlea. In this study, we examined structural analysis of the epithelial cells, gene expressions of Na+-K+-ATPase (Atp1A1) and Kir4.1 (Kcnj10), and protein localizations of Na+-K+-ATPase and Kir4.1, in the TV. Tight junctional structures were observed between epithelial dark cells and light cells in the TV. Both Atp1a1 and Kcnj10 genes were detected in the TV. In addition, immunopositive signals for both Na+-K+-ATPase and Kir4.1 were recognized in the TV. These results indicate that Na+-K+-ATPase and Kir4.1 play roles in maintaining the EP and high K+ concentration of the endolymph. Further studies are needed to clarify the physiological functions of the TV. This is the first report which demonstrates that gene and protein expression data contribute to the avian inner ear homeostasis.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2017-04
Volume volume33
Start Page 26
End Page 30
language 日本語
File Version publisher
Title Alternative Cytoskeletal elements in an acoelomorph worm, Praesagittifera naikaiensis
FullText URL poalas_034_021_180706.pdf
Author Ikeda, Risa| Fujiwara, Chiho| Hamada, Mayuko| Sakamoto, Tatsuya| Saito, Noboru| Ando, Motonori|
Abstract Acoel flatworms can move in a variety of ways such as muscular and ciliary movements via cytoskeletal elements and their neural regulations. However, those locomotive mechanisms have not yet been fully elucidated. In this study, we examined the distribution of cytoskeletal elements including filamentous actin (F-actin) and tubulin, and the neuroanatomical organization in an acoelomorph worm, Praesagittifera naikaiensis (P. naikaiensis). Video microscopy revealed the elongation/contraction and the bending/rotation processes, and the ciliary gliding movement of P. naikaiensis. Histochemical and morphological analysis demonstrated that F-actin networks of inner longitudinal and outer circular muscle fibers were positioned along the entire surface of the body, and that the average distance between the circular muscle fibers in the contracted organism was decreased in the anterior region compared with that in the elongated organism. Electron microscopy showed dense bodies on the muscle cells of P. naikaiensis, which indicates that those muscle cells have the appearance of vertebrate smooth muscle cells. Immunohistochemical analysis revealed that -tubulin-positive signals on the ciliary microtubules had close contact with the F-actin network, and that neurite bundles labelled with anti dSap47 antibody as a neuronal marker run along the anterior-posterior body axis. These results indicate that the well-organized cytoskeletal elements and their neural control systems are preserved in P. naikaiensis, and that their mechanisms involved in those regulation systems are similar to those vertebrate systems. Further studies are needed to clarify the physiological mechanisms underlying the muscular and ciliary movements in P. naikaiensis.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2018-04
Volume volume34
Start Page 21
End Page 27
language 日本語
File Version publisher
NAID 120006469118