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Author
Ye, Wenxiu Graduate School of Environmental and Life Science, Okayama University
Ando, Eigo Graduate School of Science, Nagoya University
Rhaman, Mohammad Saidur Graduate School of Environmental and Life Science, Okayama University
Tahjib-Ul-Arif, Md Graduate School of Environmental and Life Science, Okayama University
Okuma, Eiji Graduate School of Environmental and Life Science, Okayama University
Nakamura, Yoshimasa Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Kinoshita, Toshinori Institute of Transformative Bio-Molecule, Nagoya University
Murata, Yoshiyuki Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Abstract
The glucosinolate-myrosinase system is a well-known defense system that has been shown to induce stomatal closure in Brassicales. Isothiocyanates are highly reactive hydrolysates of glucosinolates, and an isothiocyanate, allyl isothiocyanate (AITC), induces stomatal closure accompanied by elevation of free cytosolic Ca2+ concentration ([Ca2+](cyt)) in Arabidopsis. It remains unknown whether AITC inhibits light-induced stomatal opening. This study investigated the role of Ca2+ in AITC-induced stomatal closure and inhibition of light-induced stomatal opening. AITC induced stomatal closure and inhibited light-induced stomatal opening in a dose-dependent manner. A Ca2+ channel inhibitor, La3+, a Ca(2+)chelator, EGTA, and an inhibitor of Ca2+ release from internal stores, nicotinamide, inhibited AITC-induced [Ca2+](cyt) elevation and stomatal closure, but did not affect inhibition of light-induced stomatal opening. AITC activated non-selective Ca2+-permeable cation channels and inhibited inward-rectifying K+ (K-in(+)) channels in a Ca2+-independent manner. AITC also inhibited stomatal opening induced by fusicoccin, a plasma membrane H+-ATPase activator, but had no significant effect on fusicoccin-induced phosphorylation of the penultimate threonine of H+-ATPase. Taken together, these results suggest that AITC induces Ca2+ influx and Ca2+ release to elevate [Ca2+](cyt), which is essential for AITC-induced stomatal closure but not for inhibition of K-in(+) channels and light-induced stomatal opening.
Keywords
Allyl isothiocyanate
Arabidopsis
calcium channel
potassium channel
proton pump
stomatal closure
stomatal opening
Published Date
2020-02-27
Publication Title
Journal of Experimental Botany
Volume
volume71
Issue
issue10
Publisher
Oxford University Press
Start Page
2922
End Page
2932
ISSN
0022-0957
NCID
AA00697526
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© The Author(s) 2020.
File Version
publisher
PubMed ID
DOI
Web of Science KeyUT
Related Url
isVersionOf https://doi.org/10.1093/jxb/eraa073
License
http://creativecommons.org/licenses/by/4.0/
Funder Name
Japan Society for the Promotion of Science
助成番号
15H05956
267977