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ID 32071
JaLCDOI
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Author
Takahashi, Fumio
Kuramitsu, Makoto
Tokuda, Masaaki
Itano, Toshifumi
Murakami, Tetsu-Hide
Hatase, Osamu
Nishida, Isamu
Abstract

Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.

Keywords
chick growth factors
cell proliferation
growth regulation
DNA and RNA synthesis
protein synthesis
Amo Type
Article
Published Date
1979-06
Publication Title
Acta Medica Okayama
Volume
volume33
Issue
issue3
Publisher
Okayama University Medical School
Start Page
167
End Page
176
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID